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Раздел 2 569 ɍȾɄ 577.023+577.322.23 ȼɅɂəɇɂȿ ɂɈɇɈȼ ɇȺ ɋɄɈɊɈɋɌɖ ɉȿɊȿɇɈɋȺ ɗɅȿɄɌɊɈɇɈȼ ȼ ɊȿȺɄɐɂɂ ȼɈɋɋɌȺɇɈȼɅȿɇɂə ɎȿɊɊɂɐɂȺɇɂȾ-ȺɇɂɈɇȺ NADPH-ɐɂɌɈɏɊɈɆ Ɋ450 ɊȿȾɍɄɌȺɁɈɃ əɧɰɟɜɢɱ Ⱥ.ȼ., Ƚɢɥɟɩ Ⱥ.Ⱥ., ɍɫɚɧɨɜ ɋ.Ⱥ. ɂɧɫɬɢɬɭɬ ɛɢɨɨɪɝɚɧɢɱɟɫɤɨɣ ɯɢɦɢɢ ɇȺɇ Ȼɟɥɚɪɭɫɢ ɐɢɬɨɯɪɨɦ Ɋ450-ɡɚɜɢɫɢɦɵɟ ɦɨɧɨɨɤɫɢɝɟɧɚɡɧɵɟ ɫɢɫɬɟɦɵ ɹɜɥɹɸɬɫɹ ɧɚɢɛɨɥɟɟ ɢɧɬɟɧɫɢɜɧɨ ɢɫɫɥɟɞɭɟɦɵɦɢ ɜ ɧɚɫɬɨɹɳɟɟ ɜɪɟɦɹ ɮɟɪɦɟɧɬɚɬɢɜɧɵɦɢ ɫɢɫɬɟɦɚɦɢ. ȼ ɪɹɞɟ ɪɚɛɨɬ ɛɵɥɨ ɩɨɤɚɡɚɧɨ, ɱɬɨ ɩɨɜɵɲɟɧɢɟ ɢɨɧɧɨɣ ɫɢɥɵ ɪɚɫɬɜɨɪɚ, ɜ ɤɨɬɨɪɨɦ ɩɪɨɬɟɤɚɟɬ ɪɟɚɤɰɢɹ, ɫɨɩɪɨɜɨɠɞɚɸɳɚɹɫɹ ɩɟɪɟɧɨɫɨɦ ɷɥɟɤɬɪɨɧɚ, ɩɪɢɜɨɞɢɬ ɤ ɭɜɟɥɢɱɟɧɢɸ ɤɨɧɫɬɚɧɬɵ ɫɤɨɪɨɫɬɢ ɪɟɚɤɰɢɢ. ɗɬɢ ɞɚɧɧɵɟ ɢɧɬɟɪɩɪɟɬɢɪɨɜɚɥɢɫɶ ɫ ɬɨɱɤɢ ɡɪɟɧɢɹ ɷɮɮɟɤɬɢɜɧɨɫɬɢ ɛɟɥɨɤ-ɛɟɥɤɨɜɵɯ ɜɡɚɢɦɨɞɟɣɫɬɜɢɣ [1]. ȼ ɧɚɫɬɨɹɳɟɣ ɪɚɛɨɬɟ ɫ ɰɟɥɶɸ ɜɵɹɜɥɟɧɢɹ ɨɛɳɢɯ ɡɚɤɨɧɨɦɟɪɧɨɫɬɟɣ ɜɥɢɹɧɢɹ ɢɨɧɧɨɣ ɫɢɥɵ ɧɚ ɫɤɨɪɨɫɬɶ ɪɟɚɤɰɢɢ ɷɥɟɤɬɪɨɧɧɨɝɨ ɩɟɪɟɧɨɫɚ ɩɪɨɜɟɞɟɧɨ ɤɢɧɟɬɢɱɟɫɤɨɟ ɢɫɫɥɟɞɨɜɚɧɢɟ ɪɟɚɤɰɢɢ ɜɨɫɫɬɚɧɨɜɥɟɧɢɹ ɮɟɪɪɢɰɢɚɧɢɞɚɧɢɨɧɚ NADPH-ɰɢɬɨɯɪɨɦ Ɋ450-ɪɟɞɭɤɬɚɡɨɣ (CPR). Ʉɢɧɟɬɢɱɟɫɤɚɹ ɤɪɢɜɚɹ ɜɨɫɫɬɚɧɨɜɥɟɧɢɹ ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧɨɜ ɭɞɨɜɥɟɬɜɨɪɹɟɬ ɤɢɧɟɬɢɱɟɫɤɨɦɭ ɭɪɚɜɧɟɧɢɸ ɩɟɪɜɨɝɨ ɩɨɪɹɞɤɚ. Ⱦɥɹ ɨɩɪɟɞɟɥɟɧɢɹ ɩɨɪɹɞɤɚ ɪɟɚɤɰɢɢ ɩɨ CPR, ɢɡɦɟɪɹɥɢ ɤɨɧɫɬɚɧɬɭ ɫɤɨɪɨɫɬɢ ɪɟɚɤɰɢɢ ɩɟɪɜɨɝɨ ɩɨɪɹɞɤɚ ɩɨ ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧɭ ɩɪɢ ɪɚɡɥɢɱɧɵɯ ɤɨɧɰɟɧɬɪɚɰɢɹɯ ɮɟɪɦɟɧɬɚ. ȼ ɞɚɧɧɨɦ ɫɥɭɱɚɟ ɦɨɠɧɨ ɩɪɟɞɩɨɥɨɠɢɬɶ, ɱɬɨ kɤɚɠ=kɫnCPR. ȼ ɬɚɛɥɢɰɟ 1 ɩɪɢɜɟɞɟɧɵ ɡɧɚɱɟɧɢɹ ɷɤɫɩɟɪɢɦɟɧɬɚɥɶɧɨ ɩɨɥɭɱɟɧɧɵɯ kɤɚɠ ɢ ɪɚɫɫɱɢɬɚɧɧɵɯ ɤɨɧɫɬɚɧɬ ɫɤɨɪɨɫɬɟɣ ɜɬɨɪɨɝɨ ɩɨɪɹɞɤɚ k2=kɤɚɠ/ɫCPR. Ɍɚɛɥɢɰɚ 1. Ɉɩɪɟɞɟɥɟɧɢɟ ɩɨɪɹɞɤɚ ɪɟɚɤɰɢɢ ɢɡ ɤɨɧɫɬɚɧɬ ɫɤɨɪɨɫɬɟɣ ɩɫɟɜɞɨɩɟɪɜɨɝɨ ɩɨɪɹɞɤɚ. Ʉɨɧɫɬɚɧɬɚ ɫɤɨɪɨɫɬɢ Ʉɨɧɫɬɚɧɬɚ ɫɤɨɪɨɫɬɢ Ʉɨɧɰɟɧɬɪɚɰɢɹ ɩɟɪɜɨɝɨ ɩɨɪɹɞɤɚ ɩɨ ɜɬɨɪɨɝɨ ɩɨɪɹɞɤɚ, ɆNADPH-ɰɢɬɨɯɪɨɦ ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧɭ, 1 -1 ɫ Ɋ450 ɪɟɞɭɤɬɚɡɵ, ɧɆ kɤɚɠ 10-3 ɫ-1 5 1,92±0,0056 384081±1124 10 3,77±0,024 376647±2361 15 6,19±0,022 412477±1487 20 7,81±0,027 390625±1342 ɉɨɫɤɨɥɶɤɭ ɜɟɥɢɱɢɧɚ k2=kɤɚɠ/ɫCPR ɧɟ ɡɚɜɢɫɢɬ ɨɬ ɤɨɧɰɟɧɬɪɚɰɢɢ CPR, ɬɨ n=1. Ɍɚɤɢɦ ɨɛɪɚɡɨɦ, ɦɨɠɧɨ ɡɚɤɥɸɱɢɬɶ, ɱɬɨ ɪɟɚɤɰɢɹ 570 Структура и динамика молекулярных систем, 2007 г., Выпуск №1 ɜɨɫɫɬɚɧɨɜɥɟɧɢɹ ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧɚ CPR ɢɦɟɟɬ ɨɛɳɢɣ ɜɬɨɪɨɣ ɩɨɪɹɞɨɤ, ɩɟɪɜɵɣ ɩɨɪɹɞɨɤ ɩɨ CPR ɢ ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧɭ. Ɉɫɨɛɵɣ ɢɧɬɟɪɟɫ ɩɪɟɞɫɬɚɜɥɹɟɬ ɜɥɢɹɧɢɟ ɢɨɧɧɨɣ ɫɢɥɵ ɧɚ ɫɤɨɪɨɫɬɶ ɮɟɪɦɟɧɬɚɬɢɜɧɵɯ ɨɤɢɫɥɢɬɟɥɶɧɨ-ɜɨɫɫɬɚɧɨɜɢɬɟɥɶɧɵɯ ɪɟɚɤɰɢɣ. ɇɚɩɪɢɦɟɪ, ɰɢɬɨɯɪɨɦ ɫ ɜɨɫɫɬɚɧɚɜɥɢɜɚɟɬɫɹ NADPH-ɰɢɬɨɯɪɨɦ Ɋ450 ɪɟɞɭɤɬɚɡɨɣ ɬɨɥɶɤɨ ɩɪɢ ɜɵɫɨɤɢɯ ɤɨɧɰɟɧɬɪɚɰɢɹɯ ɫɨɥɟɣ ɜ ɪɚɫɬɜɨɪɟ (0,3 Ɇ). ɍɜɟɥɢɱɟɧɢɟ ɤɨɧɰɟɧɬɪɚɰɢɢ ɢɨɧɨɜ ɜ ɪɟɚɤɰɢɨɧɧɨɣ ɫɪɟɞɟ ɩɪɢɜɨɞɢɬ ɤ ɩɨɜɵɲɟɧɢɸ ɤɨɧɫɬɚɧɬ ɫɤɨɪɨɫɬɢ ɪɟɚɤɰɢɣ ɜɨɫɫɬɚɧɨɜɥɟɧɢɹ ɰɢɬɨɯɪɨɦɚ b5 CPR, ɚ ɬɚɤɠɟ NADH-ɰɢɬɨɯɪɨɦ b5 ɪɟɞɭɤɬɚɡɨɣ, ɩɨɜɵɲɚɹ ɫɤɨɪɨɫɬɶ ɩɪɟɜɪɚɳɟɧɢɹ ɫɭɛɫɬɪɚɬɨɜ ɰɢɬɨɯɪɨɦ Ɋ450-ɡɚɜɢɫɢɦɵɯ ɦɨɧɨɨɤɫɢɝɟɧɚɡ. Ⱦɚɧɧɵɣ ɮɟɧɨɦɟɧ ɞɨ ɧɚɫɬɨɹɳɟɝɨ ɜɪɟɦɟɧɢ ɩɪɟɢɦɭɳɟɫɬɜɟɧɧɨ ɢɧɬɟɪɩɪɟɬɢɪɨɜɚɥɫɹ ɜ ɪɚɦɤɚɯ ɞɢɮɮɭɡɢɨɧɧɵɯ ɩɪɟɞɫɬɚɜɥɟɧɢɣ. Ⱦɥɹ ɨɛɴɹɫɧɟɧɢɹ ɭɤɚɡɚɧɧɵɯ ɜɵɲɟ ɷɮɮɟɤɬɨɜ ɛɵɥɨ ɩɪɟɞɥɨɠɟɧɨ ɧɟɫɤɨɥɶɤɨ ɚɥɶɬɟɪɧɚɬɢɜɧɵɯ ɝɢɩɨɬɟɡ, ɩɪɟɞɩɨɥɚɝɚɸɳɢɯ, ɱɬɨ ɪɟɚɤɰɢɹ ɤɨɧɬɪɨɥɢɪɭɟɬɫɹ ɞɢɮɮɭɡɢɨɧɧɨ ɢɥɢ ɨɩɪɟɞɟɥɹɟɬɫɹ ɩɪɟɢɦɭɳɟɫɬɜɟɧɧɨ ɫɢɥɨɣ ɦɟɠɦɨɥɟɤɭɥɹɪɧɵɯ ɜɡɚɢɦɨɞɟɣɫɬɜɢɣ. Ⱦɥɹ ɬɨɝɨ, ɱɬɨɛɵ ɨɩɪɟɞɟɥɢɬɶ ɢɫɬɢɧɧɭɸ ɩɪɢɱɢɧɭ ɢɡɦɟɧɟɧɢɹ ɫɤɨɪɨɫɬɟɣ ɪɟɚɤɰɢɣ ɜ ɩɪɨɫɬɨɣ ɦɨɞɟɥɶɧɨɣ ɫɢɫɬɟɦɟ ɪɟɚɤɰɢɢ ɜɨɫɫɬɚɧɨɜɥɟɧɢɹ ɮɟɪɪɢɰɢɚɧɢɞɚ ɤɚɥɢɹ CPR, ɦɵ ɩɪɨɚɧɚɥɢɡɢɪɨɜɚɥɢ ɝɪɚɮɢɤɢ Ⱥɪɪɟɧɢɭɫɚ ɩɪɢ ɪɚɡɥɢɱɧɵɯ ɤɨɧɰɟɧɬɪɚɰɢɹɯ ɯɥɨɪɢɞɚ ɧɚɬɪɢɹ. ɉɪɢɱɢɧɨɣ ɢɡɦɟɧɟɧɢɹ ɤɨɧɫɬɚɧɬɵ ɫɤɨɪɨɫɬɢ ɪɟɚɤɰɢɢ ɹɜɥɹɟɬɫɹ ɥɢɛɨ ɢɡɦɟɧɟɧɢɟ ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɨɝɨ ɦɧɨɠɢɬɟɥɹ ɜ ɭɪɚɜɧɟɧɢɢ Ⱥɪɪɟɧɢɭɫɚ ɥɢɛɨ ɷɧɟɪɝɢɢ ɚɤɬɢɜɚɰɢɢ. ɉɪɨɜɟɞɟɧɧɨɟ ɧɚɦɢ ɢɫɫɥɟɞɨɜɚɧɢɟ ɩɨɤɚɡɚɥɨ, ɱɬɨ ɩɪɢ ɩɨɜɵɲɟɧɢɢ ɤɨɧɰɟɧɬɪɚɰɢɢ ɯɥɨɪɢɞɚ ɧɚɬɪɢɹ ɜ ɪɚɫɬɜɨɪɟ ɢɡɦɟɧɹɟɬɫɹ ɤɚɤ ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɵɣ ɦɧɨɠɢɬɟɥɶ, ɬɚɤ ɢ ɷɧɟɪɝɢɹ ɚɤɬɢɜɚɰɢɢ ɪɟɚɤɰɢɢ (ɬɚɛɥɢɰɚ 2). ȼɟɥɢɱɢɧɚ ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɨɝɨ ɦɧɨɠɢɬɟɥɹ ɫ ɭɜɟɥɢɱɟɧɢɟɦ ɤɨɧɰɟɧɬɪɚɰɢɢ ɫɨɥɢ ɭɦɟɧɶɲɚɟɬɫɹ. ɗɬɨɬ ɷɮɮɟɤɬ ɦɨɠɧɨ ɨɛɴɹɫɧɢɬɶ ɫ ɢɫɩɨɥɶɡɨɜɚɧɢɟɦ ɬɟɨɪɢɢ Ⱦɟɛɚɹ-ɏɸɤɤɟɥɹ. Ɋɟɡɭɥɶɬɚɬɨɦ ɭɜɟɥɢɱɟɧɢɹ ɤɨɧɰɟɧɬɪɚɰɢɢ ɢɨɧɨɜ ɜ ɪɚɫɬɜɨɪɟ ɹɜɥɹɟɬɫɹ ɫɧɢɠɟɧɢɟ ɚɤɬɢɜɧɨɫɬɢ ɡɚɪɹɞɨɜ ɪɟɚɝɟɧɬɨɜ [2]. Ɂɧɚɱɟɧɢɹ ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɨɝɨ ɦɧɨɠɢɬɟɥɹ ɦɨɠɧɨ ɨɰɟɧɢɬɶ ɜ ɪɚɦɤɚɯ ɦɨɞɟɥɢ ɋɦɨɥɭɯɨɜɫɤɨɝɨ-ɗɣɧɲɬɟɣɧɚ. ɉɨɥɭɱɟɧɧɵɟ ɷɤɫɩɟɪɢɦɟɧɬɚɥɶɧɨ ɡɧɚɱɟɧɢɹ ɢɡɦɟɧɹɸɬɫɹ ɜ ɢɧɬɟɪɜɚɥɟ 6ɯ1014 3ɯ1010 Ɇ1 -1 ɫ . Ʉɨɷɮɮɢɰɢɟɧɬ ɩɨɫɬɭɩɚɬɟɥɶɧɨɣ ɞɢɮɮɭɡɢɢ ɦɨɠɧɨ ɨɰɟɧɢɬɶ ɩɨ kT , ɝɞɟ k ɤɨɧɫɬɚɧɬɚ Ȼɨɥɶɰɦɚɧɚ, ɮɨɪɦɭɥɟ ɋɬɨɤɫɚ-ɗɣɧɲɬɟɣɧɚ: D 6SKR 1.38ɯ10-23 Ⱦɠ Ʉ-1; T ɬɟɦɩɟɪɚɬɭɪɚ, Ʉ; Ș ɜɹɡɤɨɫɬɶ ɪɚɫɬɜɨɪɚ; R ɪɚɞɢɭɫ ɱɚɫɬɢɰɵ. Раздел 2 571 Ɍɚɛɥɢɰɚ 2. Ɂɚɜɢɫɢɦɨɫɬɶ ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɨɝɨ ɦɧɨɠɢɬɟɥɹ, ɷɧɟɪɝɢɢ ɚɤɬɢɜɚɰɢɢ ɢ ɷɧɟɪɝɢɢ ɪɟɨɪɝɚɧɢɡɚɰɢɢ ɫɪɟɞɵ ɞɥɹ ɪɟɚɤɰɢɢ ɩɟɪɟɧɨɫɚ ɷɥɟɤɬɪɨɧɚ ɨɬ FAD CPR ɤ ɮɟɪɪɢɰɢɚɧɢɞɭ ɤɚɥɢɹ. ɗɧɟɪɝɢɹ ɗɧɟɪɝɢɹ Ln[A], P= Ʉɨɧɰɟɧɬɪɚɰɢɹ ɪɟɨɪɝɚɧɢɡɚɰɢɢ ɚɤɬɢɜɚɰɢɢ ln(Ɇ-1ɫɪɟɚɤɰɢɢ, ɫɪɟɞɵ, Aɷɤɫɩ/Aɬɟɨɪ NaCl, ɦɆ 1 ) ɤȾɠ/ɦɨɥɶ ɤȾɠ/ɦɨɥɶ 0 36±5 3.3ɯ105 57±13 351±53 50 30±2 8.1ɯ102 39±4 275±16 100 27±4 40 33±8 250±33 150 26±1 15 28±4 228±17 200 25±2 5 26±2 219±8 250 24±3 2 24±3 210±13 300 24±1 2 23±2 206±4 ȼ ɪɚɫɱɟɬɚɯ ɤɨɷɮɮɢɰɢɟɧɬɚ ɩɨɫɬɭɩɚɬɟɥɶɧɨɣ ɞɢɮɮɭɡɢɢ ɢɫɩɨɥɶɡɨɜɚɥɢɫɶ ɫɥɟɞɭɸɳɢɟ ɜɟɥɢɱɢɧɵ: T=293 K; Ș=0.001 ɉɚ ɫ; R(CPR)=3ɯ10-9 ɦ; R (ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧ)=5ɯ10-10 M. ɉɨɥɭɱɟɧɧɵɟ ɤɨɷɮɮɢɰɢɟɧɬɵ ɞɢɮɮɭɡɢɢ ɞɥɹ CPR ɢ ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧɚ ɪɚɜɧɵ 7ɯ10-11 ɢ 4ɯ10-10, ɫɨɨɬɜɟɬɫɬɜɟɧɧɨ. Ɍɟɨɪɟɬɢɱɟɫɤɭɸ ɤɨɧɫɬɚɧɬɭ ɫɤɨɪɨɫɬɢ ɛɢɦɨɥɟɤɭɥɹɪɧɨɣ ɪɟɚɤɰɢɢ, ɤɨɧɬɪɨɥɢɪɭɟɦɨɣ ɞɢɮɮɭɡɢɨɧɧɨ, ɞɥɹ ɧɟɜɡɚɢɦɨɞɟɣɫɬɜɭɸɳɢɯ ɦɨɥɟɤɭɥ ɦɨɠɧɨ ɪɚɫɫɱɢɬɚɬɶ ɫ ɢɫɩɨɥɶɡɨɜɚɧɢɟɦ . ɭɪɚɜɧɟɧɢɹ: k 2 4S (R CPR R 3 )(D CPR D 3 ) N a [Fe(CN) 6 ] [Fe(CN) 6 ] Ɍɟɨɪɟɬɢɱɟɫɤɢ ɩɨɥɭɱɟɧɧɚɹ ɜɟɥɢɱɢɧɚ ɤɨɧɫɬɚɧɬɵ ɫɤɨɪɨɫɬɢ ɛɢɦɨɥɟɤɭɥɹɪɧɨɣ ɪɟɚɤɰɢɢ, ɤɨɧɬɪɨɥɢɪɭɟɦɨɣ ɞɢɮɮɭɡɢɟɣ, ɢɥɢ, ɱɬɨ ɬɨɠɟ, ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɨɝɨ ɦɧɨɠɢɬɟɥɹ, ɪɚɜɧɚ 1.3ɯ1010 Ɇ-1ɫ-1. Ⱦɚɧɧɨɟ ɡɧɚɱɟɧɢɟ ɬɢɩɢɱɧɨ ɞɥɹ ɞɜɭɯ ɧɟɜɡɚɢɦɨɞɟɣɫɬɜɭɸɳɢɯ ɱɚɫɬɢɰ ɜ ɪɚɫɬɜɨɪɟ. ȿɫɥɢ ɠɟ ɪɚɫɫɦɚɬɪɢɜɚɬɶ ɤɨɧɫɬɚɧɬɭ ɫɤɨɪɨɫɬɢ ɞɥɹ ɞɜɭɯ ɜɡɚɢɦɨɞɟɣɫɬɜɭɸɳɢɯ ɱɚɫɬɢɰ (ɢɨɧɨɜ ɫ ɩɪɨɬɢɜɨɩɨɥɨɠɧɵɦ ɡɚɪɹɞɨɦ), ɬɨ ɤɨɧɫɬɚɧɬɚ ɫɤɨɪɨɫɬɢ ɛɢɦɨɥɟɤɭɥɹɪɧɨɣ ɪɟɚɤɰɢɢ ɛɭɞɟɬ ɢɦɟɬɶ ɩɨɪɹɞɨɤ 1011 Ɇ-1ɫ-1. ɉɚɪɚɦɟɬɪ ɫɨɝɥɚɫɨɜɚɧɢɹ ɷɤɫɩɟɪɢɦɟɧɬɚɥɶɧɵɯ ɞɚɧɧɵɯ ɫ ɬɟɨɪɢɟɣ ɫɬɨɥɤɧɨɜɟɧɢɣ Ɋ, ɪɚɜɧɵɣ ɨɬɧɨɲɟɧɢɸ ɧɚɛɥɸɞɚɟɦɵɯ ɢ ɷɤɫɩɟɪɢɦɟɧɬɚɥɶɧɨ ɪɚɫɫɱɢɬɚɧɧɵɯ ɜɟɥɢɱɢɧ Ⱥ, ɩɪɟɞɫɬɚɜɥɟɧ ɜ ɬɚɛɥɢɰɟ 2. Ⱦɚɧɧɵɣ ɩɚɪɚɦɟɬɪ, ɜ ɭɫɥɨɜɢɹɯ ɧɢɡɤɨɣ ɢɨɧɧɨɣ ɫɢɥɵ ɪɚɫɬɜɨɪɚ, ɡɧɚɱɢɬɟɥɶɧɨ ɩɪɟɜɵɲɚɸɳɢɣ ɬɟɨɪɟɬɢɱɟɫɤɢ ɪɚɫɫɱɢɬɚɧɧɭɸ ɜɟɥɢɱɢɧɭ, ɩɪɢ ɩɨɜɵɲɟɧɢɢ ɢɨɧɧɨɣ ɫɢɥɵ ɫɬɪɟɦɢɬɫɹ ɤ 1. Ɉɱɟɧɶ ɛɨɥɶɲɨɟ ɡɧɚɱɟɧɢɟ Ɋ ɩɪɢ ɧɢɡɤɨɣ ɢɨɧɧɨɣ ɫɢɥɟ ɨɛɴɹɫɧɹɟɬɫɹ ɬɟɦ, ɱɬɨ ɦɨɥɟɤɭɥɚ CPR ɩɪɟɞɫɬɚɜɥɹɟɬ ɫɨɛɨɣ ɩɨɥɢɷɥɟɤɬɪɨɥɢɬ, ɢ, ɤɪɨɦɟ ɬɨɝɨ, ɮɟɪɪɢɰɢɚɧɢɞ-ɚɧɢɨɧ ɧɟɫɟɬ ɧɚ ɫɟɛɟ ɡɧɚɱɢɬɟɥɶɧɵɣ ɨɬɪɢɰɚɬɟɥɶɧɵɣ ɡɚɪɹɞ. ɉɨɜɵɲɟɧɢɟ ɢɨɧɧɨɣ ɫɢɥɵ ɪɚɫɬɜɨɪɚ 572 Структура и динамика молекулярных систем, 2007 г., Выпуск №1 ɫɧɢɦɚɟɬ ɜɡɚɢɦɨɞɟɣɫɬɜɢɹ ɦɟɠɞɭ ɱɚɫɬɢɰɚɦɢ, ɱɬɨ ɩɪɢɜɨɞɢɬ ɤ ɫɬɪɟɦɥɟɧɢɸ ɜɟɥɢɱɢɧɵ ɩɪɟɞɷɤɫɩɨɧɟɧɰɢɚɥɶɧɨɝɨ ɦɧɨɠɢɬɟɥɹ ɤ ɜɟɥɢɱɢɧɟ, ɬɢɩɢɱɧɨɣ ɞɥɹ ɧɟɜɡɚɢɦɨɞɟɣɫɬɜɭɸɳɢɯ ɦɨɥɟɤɭɥ. ɉɪɢɱɢɧɨɣ ɭɜɟɥɢɱɟɧɢɹ ɤɨɧɫɬɚɧɬɵ ɫɤɨɪɨɫɬɢ ɪɟɚɤɰɢɢ ɹɜɥɹɟɬɫɹ ɫɧɢɠɟɧɢɟ ɚɤɬɢɜɚɰɢɨɧɧɨɝɨ ɛɚɪɶɟɪɚ. ɉɪɢ ɢɡɦɟɧɟɧɢɢ ɤɨɧɰɟɧɬɪɚɰɢɢ ɯɥɨɪɢɞɚ ɧɚɬɪɢɹ ɨɬ 0 ɞɨ 300 ɦɆ ɷɧɟɪɝɢɹ ɚɤɬɢɜɚɰɢɢ ɭɦɟɧɶɲɚɟɬɫɹ ɨɬ 57 ɞɨ 23 ɤȾɠ/ɦɨɥɶ. ɉɪɢɪɨɞɚ ɚɤɬɢɜɚɰɢɨɧɧɨɝɨ ɛɚɪɶɟɪɚ ɜ ɨɤɢɫɥɢɬɟɥɶɧɨɜɨɫɫɬɚɧɨɜɢɬɟɥɶɧɵɯ ɪɟɚɤɰɢɹɯ ɨɛɴɹɫɧɹɟɬɫɹ ɫ ɩɨɦɨɳɶɸ ɬɟɨɪɢɢ ɩɟɪɟɧɨɫɚ ɷɥɟɤɬɪɨɧɨɜ ɜ ɩɨɥɹɪɧɵɯ ɫɪɟɞɚɯ Ɋ. Ɇɚɪɤɭɫɚ. ɉɪɢɱɢɧɨɣ ɜɨɡɧɢɤɧɨɜɟɧɢɹ ɷɧɟɪɝɢɢ ɚɤɬɢɜɚɰɢɢ ɨɤɢɫɥɢɬɟɥɶɧɨ-ɜɨɫɫɬɚɧɨɜɢɬɟɥɶɧɵɯ ɪɟɚɤɰɢɣ ɹɜɥɹɟɬɫɹ ɨɪɢɟɧɬɚɰɢɨɧɧɚɹ ɩɨɥɹɪɢɡɚɰɢɹ ɫɪɟɞɵ. ɂɫɯɨɞɧɵɦ ɪɟɚɝɟɧɬɚɦ ɢ ɩɪɨɞɭɤɬɚɦ ɪɟɚɤɰɢɢ ɫɨɨɬɜɟɬɫɬɜɭɟɬ ɪɚɡɥɢɱɧɚɹ ɪɚɜɧɨɜɟɫɧɚɹ ɩɨɥɹɪɢɡɚɰɢɹ ɫɪɟɞɵ, ɢɥɢ ɧɟɪɚɜɧɨɦɟɪɧɨɟ ɪɚɫɩɨɥɨɠɟɧɢɟ ɦɨɥɟɤɭɥ ɪɚɫɬɜɨɪɢɬɟɥɹ. ɉɪɢ ɩɨɜɵɲɟɧɢɢ ɤɨɧɰɟɧɬɪɚɰɢɢ ɫɨɥɢ ɜ ɪɟɚɤɰɢɨɧɧɨɣ ɫɪɟɞɟ ɢɡɦɟɧɹɟɬɫɹ ɫɨɥɶɜɚɬɧɚɹ ɨɛɨɥɨɱɤɚ ɞɨɧɨɪɚ ɢ ɚɤɰɟɩɬɨɪɚ ɷɥɟɤɬɪɨɧɨɜ, ɱɬɨ ɩɪɢɜɨɞɢɬ ɤ ɫɧɢɠɟɧɢɸ ɷɧɟɪɝɢɢ ɪɟɨɪɝɚɧɢɡɚɰɢɢ ɫɪɟɞɵ, ɚ, ɫɨɨɬɜɟɬɫɬɜɟɧɧɨ, ɤ ɫɧɢɠɟɧɢɸ ɷɧɟɪɝɢɢ ɚɤɬɢɜɚɰɢɢ. ɗɧɟɪɝɢɹ ɪɟɨɪɝɚɧɢɡɚɰɢɢ ɜ ɬɚɛɥɢɰɟ 2 ɪɚɫɫɱɢɬɚɧɚ ɩɨ ɮɨɪɦɭɥɟ Ɇɚɪɤɭɫɚ. ɉɨɬɟɧɰɢɚɥ FAD ɛɵɥ ɩɪɢɧɹɬ ɪɚɜɧɵɦ -0,348 [3], ɮɟɪɪɢɰɢɚɧɢɞɚ +0,378. Ɍɚɤɢɦ ɨɛɪɚɡɨɦ, ɜ ɞɚɧɧɨɣ ɪɚɛɨɬɟ ɭɫɬɚɧɨɜɥɟɧɚ ɩɪɢɱɢɧɚ, ɩɨ ɤɨɬɨɪɨɣ ɧɚɥɢɱɢɟ ɜ ɪɚɫɬɜɨɪɟ ɢɨɧɨɜ ɫɨɥɟɣ ɦɨɠɟɬ ɫɭɳɟɫɬɜɟɧɧɨ ɢɡɦɟɧɹɬɶ ɫɤɨɪɨɫɬɶ ɮɟɪɦɟɧɬɚɬɢɜɧɨɣ ɨɤɢɫɥɢɬɟɥɶɧɨ-ɜɨɫɫɬɚɧɨɜɢɬɟɥɶɧɨɣ ɪɟɚɤɰɢɢ. ɉɨɤɚɡɚɧɨ, ɱɬɨ ɩɨɜɵɲɟɧɢɟ ɢɨɧɧɨɣ ɫɢɥɵ ɪɚɫɬɜɨɪɚ ɨɤɚɡɵɜɚɟɬ ɞɜɨɣɧɨɣ ɷɮɮɟɤɬ ɧɚ ɤɨɧɫɬɚɧɬɭ ɫɤɨɪɨɫɬɢ. ȼɨ-ɩɟɪɜɵɯ, ɫɧɢɠɚɟɬɫɹ ɷɮɮɟɤɬɢɜɧɨɫɬɶ ɜɡɚɢɦɨɞɟɣɫɬɜɢɹ ɪɟɚɝɟɧɬɨɜ. ȼɨ-ɜɬɨɪɵɯ, ɢɨɧɵ ɫɧɢɠɚɸɬ ɷɧɟɪɝɢɸ ɚɤɬɢɜɚɰɢɢ. ɉɨɫɤɨɥɶɤɭ ɨɩɪɟɞɟɥɹɸɳɢɦ ɮɚɤɬɨɪɨɦ ɜ ɡɧɚɱɟɧɢɢ ɤɨɧɫɬɚɧɬɵ ɫɤɨɪɨɫɬɢ, ɹɜɥɹɟɬɫɹ ɢɦɟɧɧɨ ɜɟɥɢɱɢɧɚ ɷɧɟɪɝɢɢ ɚɤɬɢɜɚɰɢɢ, ɪɟɡɭɥɶɬɢɪɭɸɳɢɦ ɷɮɮɟɤɬɨɦ ɹɜɥɹɟɬɫɹ ɩɨɜɵɲɟɧɢɟ ɤɨɧɫɬɚɧɬɵ ɫɤɨɪɨɫɬɢ ɪɟɚɤɰɢɢ. Ʌɢɬɟɪɚɬɭɪɚ 1. Voznesensky A.I., Schenkman J.B. The cytochrome P4502B4-NADPH cytochrome P450 reductase electron transfer complex is not formed by charge-pairing // J.Biol.Chem., 1992. V. 267. P. 14669-14676. 2. Voznesensky A.I., Schenkman J.B. Quantitative analyses of electrostatic interactions between NADPH-cytochrome P450 reductase and cytochrome P450 enzymes // J.Biol.Chem., 1994. V. 269. P. 15724-15731. 3. Vermilion J.L., Coon M.J. Identification of the high and low potential flavins of liver microsomal NADPH-cytochrome P-450 reductase // J. Biol.Chem., 1978. V. 253. P. 8812-8819.
