, 2009, 8, 1 I GE FC , . « » . , Ig. (Fc RI) – , . Fc RI - Dermatophagoides WRNWDVYK. pteronyssinus. . : IgE , , Abstract. Such new diagnostic direction as antibody detection with the help of peptides which are the analogues of Ig-binding site, located in the active centers of receptors for immunoglobulins is suggested in this article. High affinity receptor for immunoglobulin E (Fc RI) is a key highly specialized structure on the surface of a number of immune cells. Fragment of active Fc RI center – octapeptide WRNWDVYK has been synthesized by means of classical methods of peptide chemistry. The synthesized octapeptide’s capacity to bind specific IgE to allergen of mite Dermatophagoides pteronyssinus has been proved with the help of immunoenzyme test-system. The obtained peptide may potentially be used for diagnostic purposes. 1 , 2009, 8, 1 Key words: peptide, immunoglobulin E, IgE, high affinity receptor for immunoglobulin E, Fc RI, bronchial asthma. : , 210023, . , . , 27, , – . . . . [1]. , . (Fc RI) , – [2,3], . Fc: , , , . Fc, . 2 Fc RI: [4,5]. Fc RI (Fc RI ) , . 1988 Fc R1A [6]. 1 2 , 2009, 8, , 1 1q23 [7,8]. IgE Fc RI . Fc RI -IgE 2 . , Fc RI -IgE IgE 2 2 IgE. Fc RI [9]. Fc RI IgE , IgE. in vitro , IgE. WRNWDVYKIgE , Fc RI , . 9.0 (NCBI) – 2 « », NM_002001, (PDB) - 1f6a ( : S.C.Garman, B.A.Wurzburg, S.S.Tarchevskaya, J.P.Kinet, T.S.Jardetzky) E (Fc RI ). , , , 2 IgE. Fc RI – WRNWDVYK [10]. IgE WRNWDVYK product (Sigma, P8375). WRNWDVYK[11]. 3 , 2009, 8, 1 , A280 ,1 = 0,73, A403 ,1 ,1 = 2,275, ,1 403 = 109 000 -1 -1 0,8 – 5,2 . , . (“ +”, “ ”) 1:2. +4° . , , WRNWDVYK- Dermatophagoides pteronyssinus « . IgE DP0004. (100 PNU/ ) Dermatophagoides pteronyssinus (5000 PNU/ , . ») 0,1 100 Na(Nunc MaxiSort 468667). 18 +4 . 18 3 300 1 , . +4° WRNWDVYKDermatophagoides pteronyssinus Anti-Human IgE ( -chain specific) peroxidase conjugate (Sigma, product A9667). Anti-Human IgE ( -chain specific) peroxidase conjugate (Sigma, product 1 A9667) 2 1:10 PBS-T, . -20° . 24 4 , 2009, 8, 1 Dermatophagoides pteronyssinus. “Global Intitiative for Asthma. Global Strategy for Asthma Management and Prevention” [12] Dermatophagoides pteronyssinus. DP0004 WRNWDVYK- 1 2 • 2 20 (PBS-T) 180 20 • , 60 • 3 ; +37° ; PBS-T; • 150 WRNWDVYK, • PBS-T 1:200; 150 Human IgE Anti- , • PBS-T 1:200; 60 • 3 • 150 (Sigma, product • PBS-T; 3,3'5,5'-Tetramethyl-benzidine ( ) T8665); 60 • 37° ; +18° ; 100 ; • , -01C, », , 450 . WRNWDVYK- IgE . . , . , IgE: Fc- ; 8E/5D4, IT, BSW – 5 E411, XTE4 – C 2 ; , 2009, 8E/4F4 - 8, C 3 1 ; 8E/Le27 - 8E/5D4. C 4 , , 8E/5D4, C 2 « », IgE , -8660 . -8660 + • 1 ) WRNWDVYK- 1:100; , PBS-T ; • 2 ) 8E/4F4, PBS-T 1:100; ; • 3 ) ( , , Fc RI ) [13], PBS-T 1:100; : 1 - 180 + 20 ;3 - 120 100 ;5 + 140 20 ;2 - 160 + 80 ;4 - 80 ;7 + 120 - 40 + 40 - 100 ;6 + 160 + 180 • 30 • 3 • 100 +37° ; PBS-T; 3,3'5,5'-Tetramethyl-benzidine ; 6 + - 60 ;8 - , 2009, • 8, 1 30 • +18° ; 100 ; • , -01C, 450 . , WRNWDVYK- , , -8660 8E/5D4 C 2 IgE . 8E/4F4 ( , , Fc RI ) . ( .1). 1,800 1,600 1,400 1,200 1,000 0,800 WRNWDVYK8E/4F4 0,600 0,400 0,200 0,000 20 40 80 100 120 140 1 .1 , 7 , 160 180 , , 2009, 8, 1 , , , , - . , , . , , , . - . WRNWDVYK) 8E/5D4. 15-20 , 8 . , WRNWDVYK, . WRNWDVYK-, 8E/4F4 C 2 .1. 8 IgE ( 8E/5D4) , 2009, 8, 1 1 IgE Dermatophagoides pteronyssinus IgE WRNWDVYR% WRNWDVY K WRNWDVYK- 0,516 0,641 0,624 0,902 0,816 1,061 0,732 0,916 1,288 1,871 1,344 0,657 0,829 0,806 0,864 0,446 0,848 0,965 0,760 1,031 1,229 1,745 1,391 1,435 . - % WRNWDV YK WRNWDVY K- 62,2 79,5 72,2 201,9 96,3 110,0 96,3 88,8 104,8 107,2 96,7 45,8 . 0,887 0,938 1,552 1,050 1,571 1,012 1,216 1,832 1,405 0,711 1,396 1,616 1,386 1,260 1,323 1,155 1,532 1,077 1,285 2,012 1,719 1,177 1,495 1,675 64,0 74,5 117,3 90,9 102,5 94,0 94,7 91,1 81,7 60,4 93,4 96,4 8E/5D4 C 2 IgE . , WRNWDVYK- , WRNWDVYKC 2 IgE. 2 8E/4F4 Cε2 IgE ( 8E/5D4) , -8660 WRNWDVYK8E/4F4) - IgE 180 160 120 100 80 60 40 20 + 20 + 40 + 80 + 100 + 120 + 140 + 160 + 180 . - 0,375 0,523 0,904 1,191 1,161 1,200 1,728 1,691 . 9 0,069 0,064 0,081 0,064 0,093 0,101 0,207 0,092 0,062 0,058 0,060 0,083 0,073 0,098 0,228 0,228 , 2009, 8, 1 , IgE Dermatophagoides pteronyssinus WRNWDVYK- IgE , .2, , WRNWDVYK- IgE. 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