формирование бислоев нуклеотидов группы гуанина и цитозина

Раздел 2
595
ɍȾɄ 539.23
ɎɈɊɆɂɊɈȼȺɇɂȿ ȻɂɋɅɈȿȼ ɇɍɄɅȿɈɌɂȾɈȼ ȽɊɍɉɉɕ
ȽɍȺɇɂɇȺ ɂ ɐɂɌɈɁɂɇȺ ɇȺ Ȼɂɋ-ɐɂɄɅɂɑȿɋɄɂɏ
ɌȿɆɉɅȺɌȺɏ ZN2+-Ȼɐ1
Ɍɭɪɵɝɢɧ Ⱦ.ɋ., Ʉɚɥɢɧɢɧɚ Ɇ.Ⱥ., Ɋɚɣɬɦɚɧ Ɉ.Ⱥ., Subat M., König B.,
Ⱥɪɫɥɚɧɨɜ ȼ.ȼ.*
ɂɎɏɗ ɊȺɇ, 119991, Ɇɨɫɤɜɚ, Ʌɟɧɢɧɫɤɢɣ ɩɪɨɫɩ., 31, ɤɨɪɩ. 4
Ʉɨɨɩɟɪɚɬɢɜɧɚɹ ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɹ ɦɨɥɟɤɭɥɹɪɧɵɯ ɚɧɫɚɦɛɥɟɣ ɡɚ
ɫɱɟɬ ɜɵɫɨɤɨɫɩɟɰɢɮɢɱɧɵɯ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɜɡɚɢɦɨɞɟɣɫɬɜɢɣ ɹɜɥɹɟɬɫɹ
ɮɭɧɞɚɦɟɧɬɚɥɶɧɨɣ ɩɪɨɛɥɟɦɨɣ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɨɣ ɯɢɦɢɢ. ȼ ɞɚɧɧɨɣ ɪɚɛɨɬɟ ɷɬɚ ɡɚɞɚɱɚ ɪɟɲɟɧɚ ɫ ɩɨɦɨɳɶɸ ɫɢɧɬɟɬɢɱɟɫɤɢɯ ɞɜɭɦɟɪɧɵɯ ɬɟɦɩɥɚɬɨɜ
ɢɡ ɩɪɟɞɨɪɝɚɧɢɡɨɜɚɧɧɵɯ ɛɢɜɚɥɟɧɬɧɵɯ ɰɟɧɬɪɨɜ ɫɜɹɡɵɜɚɧɢɹ, ɤɨɬɨɪɵɟ ɡɚɞɚɸɬ ɧɚɩɪɚɜɥɟɧɢɟ ɩɪɨɰɟɫɫɨɜ ɦɨɥɟɤɭɥɹɪɧɨɝɨ ɪɚɫɩɨɡɧɚɜɚɧɢɹ ɩɚɪ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɫɨɟɞɢɧɟɧɢɣ ɧɚ ɬɚɤɨɣ ɩɥɚɧɚɪɧɨɣ ɦɚɬɪɢɰɟ.
Ⱦɜɭɦɟɪɵɟ ɬɟɦɩɥɚɬɵ ɮɨɪɦɢɪɨɜɚɥɢ ɩɭɬɟɦ ɩɟɪɟɧɨɫɚ ɦɟɬɨɞɨɦ Ʌɟɧɝɦɸɪɚ-Ȼɥɨɞɠɟɬɬ (ɅȻ) ɦɨɧɨɫɥɨɟɜ ɞɢɮɢɥɶɧɵɯ ɦɟɬɚɥɥɨɤɨɦɩɥɟɤɫɨɜ ɦɚɤɪɨɰɢɤɥɢɱɟɫɤɢɯ ɩɨɥɢɚɦɢɧɨɜ – ɩɪɨɢɡɜɨɞɧɵɯ ɰɢɤɥɟɧɚ (ɪɢɫ.1) – ɧɚ ɬɜɟɪɞɭɸ ɩɨɜɟɪɯɧɨɫɬɶ, ɫɨɞɟɪɠɚɳɭɸ ɪɚɡɪɟɠɟɧɧɵɣ ɫɚɦɨɨɪɝɚɧɢɡɨɜɚɧɧɵɣ ɦɨɧɨɫɥɨɣ (ɋȺɆ) ɢɡ ɚɥɤɚɧɬɢɨɥɚ.
ɗɬɢ ɫɨɟɞɢɧɟɧɢɹ ɫɩɨɫɨɛɧɵ ɤ
ɫɟɥɟɤɬɢɜɧɨɣ
ɤɨɨɪɞɢɧɚɰɢɢ
ɦɨɥɟɤɭɥɹɪɧɵɯ ɮɪɚɝɦɟɧɬɨɜ,
ɫɨɞɟɪɠɚɳɢɯ ɢɦɢɞɧɭɸ ɢ/ɢɥɢ
ɮɨɫɮɚɬ-ɦɨɧɨɷɮɢɪɧɭɸ ɝɪɭɩɩɵ. ɉɨɥɭɱɟɧɧɵɟ ɪɚɧɟɟ ɞɚɧɧɵɟ
ɩɨɡɜɨɥɹɸɬ ɩɪɟɞɩɨɥɨɠɢɬɶ
Ɋɢɫ.1 ɋɬɪɭɤɬɭɪɧɚɹ ɮɨɪɦɭɥɚ Zn2+-ɞɢɰɟɬɢɥɰɢɤɥɟɧɚ
ɜɨɡɦɨɠɧɨɫɬɶ ɢɫɩɨɥɶɡɨɜɚɧɢɹ
2+
2+
2+
(Zn -Ⱦɐɐ) (ɚ) ɢ ɛɢɫ-(Zn -ɰɢɤɥɟɧɚ) (Zn -Ȼɐ) (ɛ).
ɦɚɬɪɢɰ
ɛɢɫ-ɰɢɤɥɢɱɟɫɤɢɯ
ɩɪɨɢɡɜɨɞɧɵɯ ɰɢɤɥɟɧɚ ɞɥɹ ɢɧɢɰɢɢɪɨɜɚɧɢɹ ɢɟɪɚɪɯɢɱɟɫɤɨɣ ɫɛɨɪɤɢ ɪɚɡɥɢɱɧɵɯ ɦɨɥɟɤɭɥ, ɫɨɞɟɪɠɚɳɢɯ ɜ ɫɜɨɟɦ ɫɨɫɬɚɜɟ ɢɦɢɞɧɵɟ ɢɥɢ ɮɨɫɮɚɬɧɵɟ
ɝɪɭɩɩɵ, ɩɪɟɠɞɟ ɜɫɟɝɨ, ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹ ɢ ɢɯ ɩɪɨɢɡɜɨɞɧɵɟ (ɧɭɤɥɟɨɬɢɞɵ,
ɧɭɤɥɟɢɧɨɜɵɟ ɤɢɫɥɨɬɵ ɢ ɢɯ ɮɪɚɝɦɟɧɬɵ). ɉɪɢ ɷɬɨɦ ɷɮɮɟɤɬɢɜɧɨɫɬɶ ɫɜɹɡɵɜɚɧɢɹ ɤɨɦɩɨɧɟɧɬɨɜ ɫɢɫɬɟɦɵ ɡɚɜɢɫɢɬ ɨɬ ɫɬɟɩɟɧɢ ɫɬɟɪɢɱɟɫɤɨɝɨ ɫɨɨɬɜɟɬɫɬɜɢɹ ɨɛɪɚɡɭɸɳɢɯ ɟɟ ɦɨɥɟɤɭɥ. ɇɚ ɛɢɫɰɢɤɥɢɱɟɫɤɢɯ ɬɟɦɩɥɚɬɚɯ ɪɨɫɬ
1
Ɋɚɛɨɬɚ ɜɵɩɨɥɧɟɧɚ ɩɪɢ ɮɢɧɚɧɫɨɜɨɣ ɩɨɞɞɟɪɠɤɟ ɊɎɎɂ (ɝɪɚɧɬ ʋ 05-0332195 ɢ ɝɪɚɧɬ ʋ 07-03-13519-ɨɮɢ_ɰ) ɢ ɈɏɇɆ ɊȺɇ (ʋ7)
596
Структура и динамика молекулярных систем, 2007 г., Выпуск №1
ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɨɣ ɫɬɪɭɤɬɭɪɵ ɩɪɨɬɟɤɚɟɬ ɩɨ «ɤɚɫɤɚɞɧɨɣ» ɫɯɟɦɟ: ɤɨɨɪɞɢɧɚɰɢɨɧɧɨɟ ɫɜɹɡɵɜɚɧɢɟ ɬɟɦɩɥɚɬɚ ɫ ɫɭɛɫɬɪɚɬɨɦ (ɧɚɩɪɢɦɟɪ, ɩɪɨɢɡɜɨɥɶɧɵɦ ɧɭɤɥɟɨɬɢɞɨɦ) (ɪɢɫ.2ɚ) – ɜɡɚɢɦɨɞɟɣɫɬɜɢɟ ɫɜɹɡɚɧɧɨɝɨ ɫ ɬɟɦɩɥɚɬɨɦ
ɧɭɤɥɟɨɬɢɞɚ ɢ ɟɝɨ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɩɚɪɬɧɟɪɚ ɢɡ ɪɚɫɬɜɨɪɚ ɡɚ ɫɱɟɬ ɨɛɪɚɡɨɜɚɧɢɹ ɜɨɞɨɪɨɞɧɵɯ ɫɜɹɡɟɣ ɦɟɠɞɭ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹɦɢ ɢ ɫɬɟɪɢɱɟɫɤɢɨɛɭɫɥɨɜɥɟɧɧɚɹ ɨɪɢɟɧɬɚɰɢɹ ɤɨɧɰɟɜɨɣ ɮɭɧɤɰɢɨɧɚɥɶɧɨɣ ɝɪɭɩɩɵ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɧɚ ɬɟɦɩɥɚɬ (ɪɢɫ.2.ɛ) – ɤɨɨɪɞɢɧɚɰɢɨɧɧɨɟ ɫɜɹɡɵɜɚɧɢɟ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɫ ɬɟɦɩɥɚɬɨɦ (ɪɢɫ.2.ɜ) – ɪɚɫɩɨɡɧɚɜɚɧɢɟ ɫɨɨɬɜɟɬɫɬɜɭɸɳɢɯ ɧɭɤɥɟɨɬɢɞɨɜ ɢɡ ɪɚɫɬɜɨɪɚ ɩɭɬɟɦ ɨɛɪɚɡɨɜɚɧɢɹ
ɜɨɞɨɪɨɞɧɵɯ ɫɜɹɡɟɣ ɫ ɡɚɤɪɟɩɥɟɧɧɨɣ ɧɚ ɬɟɦɩɥɚɬɟ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɣ ɩɚɪɨɣ
ɧɭɤɥɟɨɬɢɞɨɜ (ɪɢɫ.2.ɝ). ȼ ɪɟɡɭɥɶɬɚɬɟ ɮɨɪɦɢɪɭɸɬɫɹ ɝɟɬɟɪɨɝɟɧɧɵɟ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɟ ɛɢɫɥɨɢ, ɜ ɤɨɬɨɪɵɯ ɤɚɠɞɵɣ ɫɦɟɲɚɧɧɵɣ ɦɨɧɨɫɥɨɣ ɫɨɞɟɪɠɢɬ ɪɚɜɧɨɟ ɱɢɫɥɨ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɣ [1].
ɋɨɝɥɚɫɧɨ ɩɪɢɜɟɞɟɧɧɨɦɭ
ɜɵɲɟ ɦɟɯɚɧɢɡɦɭ, ɮɨɪɦɢɪɨɜɚɧɢɟ ɝɨɦɨɝɟɧɧɵɯ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɛɢɫɥɨɟɜ, ɜ ɤɨɬɨɪɵɯ
ɤɚɠɞɵɣ ɦɨɧɨɫɥɨɣ ɫɨɞɟɪɠɢɬ
ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹ ɬɨɥɶɤɨ ɨɞɧɨɝɨ ɬɢɩɚ, ɧɟɜɨɡɦɨɠɧɨ.
Ⱦɥɹ ɪɟɚɥɢɡɚɰɢɢ ɫɚɦɨɫɛɨɪɤɢ ɝɨɦɨɝɟɧɧɵɯ ɛɢɫɥɨɟɜ
ɛɵɥɢ ɫɮɨɪɦɢɪɨɜɚɧɵ ɬɟɦɩɥɚɬɵ
ɢɡ ɦɨɧɨɰɢɤɥɢɱɟɫɤɨɝɨ ɩɪɨɢɡɜɨɞɧɨɝɨ (Zn2+-Ⱦɐɐ).
ɉɨɫɥɟɞɨɜɚɬɟɥɶɧɚɹ
ɚɞɊɢɫ.2. «Ʉɚɫɤɚɞɧɚɹ» ɫɯɟɦɚ ɪɨɫɬɚ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɨɣ ɫɬɪɭɤɬɭɪɵ.
ɫɨɪɛɰɢɹ
ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ
ɧɭɤɥɟɨɬɢɞɨɜ (ɭɪɢɞɢɧɮɨɫɮɚɬɨɜ
5’-ɍɏɎ ɢ ɚɞɟɧɨɡɢɧɮɨɫɮɚɬɨɜ 5’-ȺɏɎ, ɝɞɟ ɏ – ɱɢɫɥɨ ɮɨɫɮɚɬɧɵɯ ɝɪɭɩɩ)
ɧɚ ɋȺɆ-ɩɥɟɧɤɚɯ ɅȻ ɢɡ Zn2+-Ⱦɐɐ ɛɵɥɚ ɢɫɫɥɟɞɨɜɚɧɚ ɦɟɬɨɞɨɦ ɩɨɜɟɪɯɧɨɫɬɧɨɝɨ ɩɥɚɡɦɨɧɧɨɝɨ ɪɟɡɨɧɚɧɫɚ (ɉɉɊ). Ʉɢɧɟɬɢɤɚ ɫɜɹɡɵɜɚɧɢɹ ɧɭɤɥɟɨɡɢɞ
ɦɨɧɨɮɨɫɮɚɬɨɜ ɧɚ ɬɚɤɨɣ ɩɨɜɟɪɯɧɨɫɬɢ ɢ ɫɨɨɬɜɟɬɫɬɜɭɸɳɢɟ ɉɉɊ-ɨɬɤɥɢɤɢ
ɫɯɨɞɧɵ ɞɥɹ ɨɛɟɢɯ ɫɬɚɞɢɣ ɚɞɫɨɪɛɰɢɢ ɢ ɧɟ ɡɚɜɢɫɹɬ ɨɬ ɩɨɪɹɞɤɚ ɜɜɟɞɟɧɢɹ
ɧɭɤɥɟɨɬɢɞɨɜ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ. Ȼɵɥ ɩɪɟɞɥɨɠɟɧ ɦɟɯɚɧɢɡɦ ɫɬɚɞɢɣɧɨɣ ɚɞɫɨɪɛɰɢɢ ɧɭɤɥɟɨɬɢɞɨɜ ɧɚ ɦɨɧɨɰɢɤɥɢɱɟɫɤɨɦ ɬɟɦɩɥɚɬɟ ɩɨ ɫɬɭɩɟɧɱɚɬɨɦɭ ɦɨɧɨɜɚɥɟɧɬɧɨɦɭ ɦɟɯɚɧɢɡɦɭ. Ɉɞɢɧ ɢɡ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɩɚɪɬɧɟɪɨɜ
ɤɨɨɪɞɢɧɢɪɭɟɬɫɹ ɧɚ Zn2+-Ⱦɐɐ ɡɚ ɫɱɟɬ ɫɜɹɡɵɜɚɧɢɹ ɤɨɧɰɟɜɨɣ ɮɨɫɮɚɬɧɨɣ
ɝɪɭɩɩɵ, ɮɨɪɦɢɪɭɹ ɬɟɦɩɥɚɬɧɭɸ ɦɚɬɪɢɰɭ ɫ ɨɫɧɨɜɚɧɢɹɦɢ, ɷɤɫɩɨɧɢɪɨɜɚɧɧɵɦɢ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ. ɉɨɫɥɟɞɭɸɳɟɟ ɪɚɫɩɨɡɧɚɜɚɧɢɟ ɨɫɭɳɟɫɬɜɥɹɟɬɫɹ ɡɚ ɫɱɟɬ ɜɵɫɨɤɨɫɩɟɰɢɮɢɱɟɫɤɨɝɨ ɨɛɪɚɡɨɜɚɧɢɹ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɩɚɪ
Раздел 2
597
ɦɟɠɞɭ ɨɫɧɨɜɚɧɢɹɦɢ ɫɨɨɬɜɟɬɫɬɜɭɸɳɢɯ ɧɭɤɥɟɨɬɢɞɨɜ ɬɟɦɩɥɚɬɧɨɣ ɦɚɬɪɢɰɵ ɢ ɧɭɤɥɟɨɬɢɞɨɜ ɢɡ ɤɨɧɬɚɤɬɧɨɝɨ ɪɚɫɬɜɨɪɚ. ȼ ɪɟɡɭɥɶɬɚɬɟ ɮɨɪɦɢɪɭɸɬɫɹ
ɝɨɦɨɝɟɧɧɵɟ ɛɢɫɥɨɢ ɧɭɤɥɟɨɬɢɞɨɜ, ɜ ɤɨɬɨɪɵɯ ɤɚɠɞɵɣ ɦɨɧɨɫɥɨɣ ɫɨɞɟɪɠɢɬ
ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹ ɬɨɥɶɤɨ ɨɞɧɨɝɨ ɬɢɩɚ (ɪɢɫ. 3) [2].
Ɍɚɤɢɦ ɨɛɪɚɡɨɦ, ɦɟɯɚɧɢɡɦ ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɢ ɩɥɚɧɚɪɧɨɣ ɫɢɫɬɟɦɵ («ɞɜɨɣɧɨɣ
ɩɥɨɫɤɨɫɬɢ» ɢɡ ɧɭɤɥɟɨɬɢɞɨɜ) ɧɚɩɪɹɦɭɸ ɨɩɪɟɞɟɥɹɟɬɫɹ ɦɨɥɟɤɭɥɹɪɧɨɣ ɝɟɨɦɟɬɪɢɟɣ ɬɟɦɩɥɚɬɚ ɢ ɫɬɪɨɟɧɢɟɦ ɪɚɫɩɨɡɧɚɸɳɟɝɨ ɦɚɤɪɨɰɢɤɥɚ-ɪɟɰɟɩɬɨɪɚ.
Ⱦɥɹ ɜɵɹɜɥɟɧɢɹ ɡɚɤɨɧɨɦɟɪɧɨɫɬɟɣ ɩɪɢ
ɮɨɪɦɢɪɨɜɚɧɢɢ ɝɟɬɟɪɨɝɟɧɧɵɯ ɛɢɫɥɨɟɜ, ɛɵɥɚ
ɨɫɭɳɟɫɬɜɥɟɧɚ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɚɹ ɫɚɦɨɫɛɨɪɊɢɫ.3 ɋɯɟɦɚ ɫɬɪɭɤɬɭɪɵ ɝɨɦɨ- ɤɚ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɬɢɞɨɜ ɫɟɦɟɣɝɟɧɧɵɯ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɛɢ- ɫɬɜɚ ɝɭɚɧɢɧɚ ɢ ɰɢɬɨɡɢɧɚ (5’-ȽɌɎ ɢ 5’ɫɥɨɟɜ ɧɭɤɥɟɨɬɢɞɨɜ, ɮɨɪɦɢɪɭɸɳɢɯɫɹ ɜ ɪɟɡɭɥɶɬɚɬɟ ɫɬɚɞɢɣɧɨɣ ɐɌɎ). Ʉɢɧɟɬɢɱɟɫɤɚɹ ɤɪɢɜɚɹ ɫɬɚɞɢɣɧɨɣ ɚɞɚɞɫɨɪɛɰɢɢ ɧɚ ɦɨɧɨɰɢɤɥɢɱɟɫɤɨɦ ɫɨɪɛɰɢɢ ɞɥɹ ɷɬɢɯ ɧɭɤɥɟɨɬɢɞɨɜ ɩɪɟɞɫɬɚɜɥɟɬɟɦɩɥɚɬɟ.
ɧɚ ɧɚ ɪɢɫ.4. ɋɨɝɥɚɫɧɨ ɤɪɢɜɨɣ, ɚɧɚɥɨɝɢɱɧɨɣ
ɩɨ ɮɨɪɦɟ ɤɢɧɟɬɢɱɟɫɤɨɣ ɤɪɢɜɨɣ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɝɨ ɫɜɹɡɵɜɚɧɢɹ 5’-ȺɌɎ ɢ 5’-ɍɌɎ, ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɹ ɝɭɚɧɢɞɢɧɨɜɵɯ
ɢ ɰɢɬɨɡɢɧɨɜɵɯ ɧɭɤɥɟɨɬɢɞɨɜ ɩɪɢɜɨɞɢɬ ɤ ɨɛɪɚɡɨɜɚɧɢɸ ɝɟɬɟɪɨɝɟɧɧɨɝɨ
ɛɢɫɥɨɹ ɩɨ ɤɨɨɩɟɪɚɬɢɜɧɨɦɭ ɦɟɯɚɧɢɡɦɭ.
Ʉɪɨɦɟ ɬɨɝɨ, ɭɫɩɟɲɧɨɟ ɮɨɪɦɢɪɨɜɚɧɢɟ
ɬɚɤɨɣ ɫɢɫɬɟɦɵ ɜɨɡɦɨɠɧɨ ɬɨɥɶɤɨ ɩɪɢ
ɢɧɢɰɢɢɪɨɜɚɧɢɢ ɚɞɫɨɪɛɰɢɢ ɰɢɬɢɞɢɧɨɜɵɦ ɧɭɤɥɟɨɬɢɞɨɦ. ɇɚɱɚɥɶɧɚɹ
ɚɞɫɨɪɛɰɢɹ ɝɭɚɧɢɞɢɧɨɜɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɨɤɚɡɚɥɚɫɶ ɧɟɷɮɮɟɤɬɢɜɧɨɣ, ɢ ɦɨɞɢɮɢɰɢɪɨɜɚɧɧɚɹ ɷɬɢɦ ɧɭɤɥɟɨɬɢɞɨɦ
ɩɨɜɟɪɯɧɨɫɬɶ ɨɛɥɚɞɚɥɚ ɧɢɡɤɨɣ ɚɤɬɢɜɧɨɫɬɶɸ ɩɨ ɨɬɧɨɲɟɧɢɸ ɤ ɤɨɦɩɥɟɦɟɧɊɢɫ.4 ɋɟɧɫɨɝɪɚɦɦɚ ɉɉɊ ɞɥɹ ɬɟɦɩɥɚɬɨɜ ɬɚɪɧɨɦɭ ɩɚɪɬɧɟɪɭ. ȼɟɪɨɹɬɧɨɣ ɩɪɢZn2+-Ȼɐ ɩɪɢ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɦ ɞɨɛɚɜɥɟ- ɱɢɧɨɣ ɷɬɨɝɨ ɷɮɮɟɤɬɚ ɦɨɠɟɬ ɹɜɥɹɬɶɫɹ
ɧɢɢ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ ɰɢɬɢɞɢɧɮɨɫɮɚɬɚ/ɝɭɚɧɨɡɢɧɮɨɫɮɚɬɚ /ɰɢɬɢɞɢɧɮɨɫɮɚɬɚ ɧɢɡɤɚɹ ɫɬɚɛɢɥɶɧɨɫɬɶ ɝɭɚɧɢɞɢɧɨɜɵɯ
(ɩɨɥɧɚɹ ɬɪɟɯɫɬɚɞɢɣɧɚɹ ɤɨɨɩɟɪɚɬɢɜɧɚɹ ɧɭɤɥɟɨɬɢɞɨɜ ɜ ɜɨɞɧɵɯ ɪɚɫɬɜɨɪɚɯ ɩɪɢ
ɫɛɨɪɤɚ); ɤɨɧɰɟɧɬɪɚɰɢɢ ɧɭɤɥɟɨɬɢɞɨɜ 0.1 ɞɚɧɧɵɯ ɪɇ, ɩɪɢɜɨɞɹɳɚɹ ɤ ɛɵɫɬɪɨɦɭ
ɝ/ɥ, ɪɇ 7.55.
ɝɢɞɪɨɥɢɡɭ ɫɜɹɡɚɧɧɨɝɨ ɫ ɦɚɬɪɢɰɟɣ
ȽɌɎ. ȼ ɪɟɡɭɥɶɬɚɬɟ ɝɢɞɪɨɥɢɬɢɱɟɫɤɨɝɨ ɪɚɫɳɟɩɥɟɧɢɹ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɟ
ɭɯɨɞɢɬ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ, ɨɫɬɚɜɥɹɹ ɧɚ ɩɨɜɟɪɯɧɨɫɬɢ ɪɢɛɨɡɨ-5ɮɨɫɮɚɬ, ɧɟɫɩɨɫɨɛɧɵɣ ɤ ɚɞɫɨɪɛɰɢɢ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ.
598
Структура и динамика молекулярных систем, 2007 г., Выпуск №1
Ⱦɥɹ ɩɨɞɬɜɟɪɠɞɟɧɢɹ ɦɟɯɚɧɢɡɦɚ ɤɨɨɩɟɪɚɬɢɜɧɨɣ ɫɚɦɨɫɛɨɪɤɢ, ɜ ɱɚɫɬɧɨɫɬɢ, ɨɛɪɚɡɨɜɚɧɢɹ ɫɬɪɭɤɬɭɪɵ ɫ ɭɱɚɫɬɢɟɦ ɫɩɟɰɢɮɢɱɟɫɤɢɯ ɜɨɞɨɪɨɞɧɵɯ
ɜɡɚɢɦɨɞɟɣɫɬɜɢɣ ɜ ɜɨɞɧɨɣ ɫɪɟɞɟ, ɛɵɥɢ ɩɪɨɜɟɞɟɧɵ ɢɫɫɥɟɞɨɜɚɧɢɹ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɣ ɚɞɫɨɪɛɰɢɢ ɧɟɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɫɨɱɟɬɚɧɢɣ ɧɭɤɥɟɨɬɢɞɨɜ.
Ʉɚɤ ɜɢɞɧɨ ɢɡ ɪɢɫ.5, ɛɢɫɰɢɤɥɢɱɟɫɤɢɣ ɬɟɦɩɥɚɬ ɫ ɩɪɟɞɜɚɪɢɬɟɥɶɧɨ ɢɦɦɨɛɢɥɢɡɨɜɚɧɧɵɦ ɰɢɬɢɞɢɧɨɜɵɦ ɧɭɤɥɟɨɬɢɞɨɦ (ɐɌɎ) ɩɢɪɢɦɢɞɢɧɨɜɨɝɨ ɪɹɞɚ ɧɟ
ɫɩɨɫɨɛɟɧ ɤ ɫɩɟɰɢɮɢɱɟɫɤɨɣ ɚɞɫɨɪɛɰɢɢ ɧɟɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ
ɩɭɪɢɧɨɜɨɝɨ ɪɹɞɚ (ȺɌɎ). ȼ ɨɬɥɢɱɢɟ ɨɬ ɤɨɨɩɟɪɚɬɢɜɧɨɝɨ ɪɚɫɩɨɡɧɚɜɚɧɢɹ
ɫɢɫɬɟɦɵ ɐɌɎ/ȽɌɎ ɫ ɞɜɭɤɪɚɬɧɵɦ ɜɬɨɪɢɱɧɵɦ ɨɬɤɥɢɤɨɦ ɉɉɊ ɨɬɧɨɫɢɬɟɥɶɧɨ ɨɬɤɥɢɤɚ ɧɚ ɩɟɪɜɨɣ ɫɬɚɞɢɢ (ɫɦ. ɪɢɫ.4), ɜ ɞɚɧɧɨɦ ɫɥɭɱɚɟ ɧɚɛɥɸɞɚɟɬɫɹ ɧɟɫɩɟɰɢɮɢɱɟɫɤɚɹ ɚɞɫɨɪɛɰɢɹ ɫ ɨɬɤɥɢɤɨɦ ɜ ɞɜɚ ɪɚɡɚ ɦɟɧɶɲɢɦ, ɱɟɦ
ɨɬɤɥɢɤ ɩɪɢ ɩɟɪɜɢɱɧɨɣ ɚɞɫɨɪɛɰɢɢ ɐɌɎ. Ȼɨɥɟɟ ɬɨɝɨ, ɧɟɫɩɟɰɢɮɢɱɟɫɤɢ
ɚɞɫɨɪɛɢɪɨɜɚɧɧɵɣ ȺɌɎ ɩɨɥɧɨɫɬɶɸ ɭɞɚɥɹɟɬɫɹ ɫ ɩɨɜɟɪɯɧɨɫɬɢ ɐɌɎɬɟɦɩɥɚɬ ɩɪɢ ɩɪɨɦɵɜɚɧɢɢ ɉɉɊ-ɹɱɟɣɤɢ ɱɢɫɬɨɣ ɜɨɞɨɣ.
Ɍɚɤɢɦ ɨɛɪɚɡɨɦ, ɦɟɯɚɧɢɡɦ
ɤɨɨɩɟɪɚɬɢɜɧɨɣ ɫɛɨɪɤɢ ɫɬɪɨɝɨ
ɫɩɟɰɢɮɢɱɟɧ, ɚ ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɹ
ɩɥɚɧɚɪɧɨɣ ɛɢɫɥɨɣɧɨɣ ɫɬɪɭɤɬɭɪɵ ɧɚ ɦɚɤɪɨɰɢɤɥɢɱɟɫɤɢɯ
ɬɟɦɩɥɚɬɚɯ ɜɨɡɦɨɠɧɚ ɬɨɥɶɤɨ ɢɡ
ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɬɢɞɨɜ.
ɋɥɟɞɭɟɬ ɨɬɦɟɬɢɬɶ, ɱɬɨ ɢɫɫɥɟɞɨɜɚɧɧɵɣ
ɩɪɨɰɟɫɫ
ɫɚɦɨɨɪɊɢɫ.5 ɋɟɧɫɨɝɪɚɦɦɚ ɉɉɊ ɞɥɹ ɬɟɦɩɥɚɬɨɜ Zn2+ɝɚɧɢɡɚɰɢɢ
ɹɜɥɹɟɬɫɹ
ɨɞɧɢɦ
ɢɡ
Ȼɐ ɩɪɢ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɦ ɞɨɛɚɜɥɟɧɢɢ ɜ
ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ ɰɢɬɢɞɢɧɮɨɫɮɚɬɚ ɢ ɚɞɟɧɨ- ɪɟɞɱɚɣɲɢɯ ɩɪɢɦɟɪɨɜ ɤɨɨɩɟɪɚɡɢɧɮɨɫɮɚɬɚ ɫ ɩɨɫɥɟɞɭɸɳɢɦ ɩɪɨɦɵɜɚɧɢɟɦ ɬɢɜɧɨɣ ɫɛɨɪɤɢ ɢɫɤɭɫɫɬɜɟɧɧɵɯ
ɤɨɧɬɚɤɬɧɨɣ ɹɱɟɣɤɢ ɱɢɫɬɨɣ ɜɨɞɨɣ (ɩɨɥɧɚɹ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɵɯ ɫɢɫɬɟɦ ɧɚ
ɬɪɟɯɫɬɚɞɢɣɧɚɹ ɤɨɨɩɟɪɚɬɢɜɧɚɹ ɫɛɨɪɤɚ); ɤɨɧɰɟɧɬɜɟɪɞɵɯ ɩɨɜɟɪɯɧɨɫɬɹɯ. Ʉɪɨɦɟ
ɬɪɚɰɢɢ ɧɭɤɥɟɨɬɢɞɨɜ 0.1 ɝ/ɥ, ɪɇ 7.55.
ɬɨɝɨ, ɩɪɟɞɥɨɠɟɧɧɵɣ ɩɨɞɯɨɞ
ɩɨɡɜɨɥɹɟɬ ɩɪɟɨɞɨɥɟɜɚɬɶ ɨɝɪɚɧɢɱɟɧɢɟ, ɧɚɥɚɝɚɟɦɨɟ ɧɚ ɫɚɦɭ ɜɨɡɦɨɠɧɨɫɬɶ
ɫɢɧɬɟɡɚ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɵɯ ɫɬɪɭɤɬɭɪ ɩɨɫɪɟɞɫɬɜɨɦ ɜɨɞɨɪɨɞɧɵɯ ɫɜɹɡɟɣ ɜ
ɜɨɞɧɨɣ ɫɪɟɞɟ, ɡɚ ɫɱɟɬ ɫɩɟɰɢɮɢɱɟɫɤɨɣ ɝɟɨɦɟɬɪɢɢ ɬɟɦɩɥɚɬɚ ɢ ɢɫɩɨɥɶɡɨɜɚɧɢɹ ɫɨɜɨɤɭɩɧɨɫɬɢ ɮɢɡɢɤɨ-ɯɢɦɢɱɟɫɤɢɯ ɫɜɨɣɫɬɜ ɞɜɭɦɟɪɧɵɯ ɫɢɫɬɟɦ ɧɚ
ɝɪɚɧɢɰɟ ɪɚɡɞɟɥɚ ɮɚɡ.
Ʌɢɬɟɪɚɬɭɪɚ:
1. Turygin D.S., Subat M., Raitman O.A., Arslanov V.V., K nig B., Kalinina M.A. // Cooperative Self-Assembly of Adenosine and Uridine Nucleotides on a 2D Synthetic Template //
Angewandte Chemie Int. Ed. 2006, 45, 5340 –5344.
2. D.S. Turygin, M. Subat, O.A. Raitman, S.L. Selector, V.V. Arslanov, B. König, M.A. Kalinina // Two-dimensional Arrays of Amphiphilic Zn2+-cyclens for Guided Molecular Recognition at Interfaces // Langmuir, 2007, 23, 2517-2524.