формирование бислоев нуклеотидов группы гуанина и цитозина
реклама
Раздел 2 595 ɍȾɄ 539.23 ɎɈɊɆɂɊɈȼȺɇɂȿ ȻɂɋɅɈȿȼ ɇɍɄɅȿɈɌɂȾɈȼ ȽɊɍɉɉɕ ȽɍȺɇɂɇȺ ɂ ɐɂɌɈɁɂɇȺ ɇȺ Ȼɂɋ-ɐɂɄɅɂɑȿɋɄɂɏ ɌȿɆɉɅȺɌȺɏ ZN2+-Ȼɐ1 Ɍɭɪɵɝɢɧ Ⱦ.ɋ., Ʉɚɥɢɧɢɧɚ Ɇ.Ⱥ., Ɋɚɣɬɦɚɧ Ɉ.Ⱥ., Subat M., König B., Ⱥɪɫɥɚɧɨɜ ȼ.ȼ.* ɂɎɏɗ ɊȺɇ, 119991, Ɇɨɫɤɜɚ, Ʌɟɧɢɧɫɤɢɣ ɩɪɨɫɩ., 31, ɤɨɪɩ. 4 Ʉɨɨɩɟɪɚɬɢɜɧɚɹ ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɹ ɦɨɥɟɤɭɥɹɪɧɵɯ ɚɧɫɚɦɛɥɟɣ ɡɚ ɫɱɟɬ ɜɵɫɨɤɨɫɩɟɰɢɮɢɱɧɵɯ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɜɡɚɢɦɨɞɟɣɫɬɜɢɣ ɹɜɥɹɟɬɫɹ ɮɭɧɞɚɦɟɧɬɚɥɶɧɨɣ ɩɪɨɛɥɟɦɨɣ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɨɣ ɯɢɦɢɢ. ȼ ɞɚɧɧɨɣ ɪɚɛɨɬɟ ɷɬɚ ɡɚɞɚɱɚ ɪɟɲɟɧɚ ɫ ɩɨɦɨɳɶɸ ɫɢɧɬɟɬɢɱɟɫɤɢɯ ɞɜɭɦɟɪɧɵɯ ɬɟɦɩɥɚɬɨɜ ɢɡ ɩɪɟɞɨɪɝɚɧɢɡɨɜɚɧɧɵɯ ɛɢɜɚɥɟɧɬɧɵɯ ɰɟɧɬɪɨɜ ɫɜɹɡɵɜɚɧɢɹ, ɤɨɬɨɪɵɟ ɡɚɞɚɸɬ ɧɚɩɪɚɜɥɟɧɢɟ ɩɪɨɰɟɫɫɨɜ ɦɨɥɟɤɭɥɹɪɧɨɝɨ ɪɚɫɩɨɡɧɚɜɚɧɢɹ ɩɚɪ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɫɨɟɞɢɧɟɧɢɣ ɧɚ ɬɚɤɨɣ ɩɥɚɧɚɪɧɨɣ ɦɚɬɪɢɰɟ. Ⱦɜɭɦɟɪɵɟ ɬɟɦɩɥɚɬɵ ɮɨɪɦɢɪɨɜɚɥɢ ɩɭɬɟɦ ɩɟɪɟɧɨɫɚ ɦɟɬɨɞɨɦ Ʌɟɧɝɦɸɪɚ-Ȼɥɨɞɠɟɬɬ (ɅȻ) ɦɨɧɨɫɥɨɟɜ ɞɢɮɢɥɶɧɵɯ ɦɟɬɚɥɥɨɤɨɦɩɥɟɤɫɨɜ ɦɚɤɪɨɰɢɤɥɢɱɟɫɤɢɯ ɩɨɥɢɚɦɢɧɨɜ ɩɪɨɢɡɜɨɞɧɵɯ ɰɢɤɥɟɧɚ (ɪɢɫ.1) ɧɚ ɬɜɟɪɞɭɸ ɩɨɜɟɪɯɧɨɫɬɶ, ɫɨɞɟɪɠɚɳɭɸ ɪɚɡɪɟɠɟɧɧɵɣ ɫɚɦɨɨɪɝɚɧɢɡɨɜɚɧɧɵɣ ɦɨɧɨɫɥɨɣ (ɋȺɆ) ɢɡ ɚɥɤɚɧɬɢɨɥɚ. ɗɬɢ ɫɨɟɞɢɧɟɧɢɹ ɫɩɨɫɨɛɧɵ ɤ ɫɟɥɟɤɬɢɜɧɨɣ ɤɨɨɪɞɢɧɚɰɢɢ ɦɨɥɟɤɭɥɹɪɧɵɯ ɮɪɚɝɦɟɧɬɨɜ, ɫɨɞɟɪɠɚɳɢɯ ɢɦɢɞɧɭɸ ɢ/ɢɥɢ ɮɨɫɮɚɬ-ɦɨɧɨɷɮɢɪɧɭɸ ɝɪɭɩɩɵ. ɉɨɥɭɱɟɧɧɵɟ ɪɚɧɟɟ ɞɚɧɧɵɟ ɩɨɡɜɨɥɹɸɬ ɩɪɟɞɩɨɥɨɠɢɬɶ Ɋɢɫ.1 ɋɬɪɭɤɬɭɪɧɚɹ ɮɨɪɦɭɥɚ Zn2+-ɞɢɰɟɬɢɥɰɢɤɥɟɧɚ ɜɨɡɦɨɠɧɨɫɬɶ ɢɫɩɨɥɶɡɨɜɚɧɢɹ 2+ 2+ 2+ (Zn -Ⱦɐɐ) (ɚ) ɢ ɛɢɫ-(Zn -ɰɢɤɥɟɧɚ) (Zn -Ȼɐ) (ɛ). ɦɚɬɪɢɰ ɛɢɫ-ɰɢɤɥɢɱɟɫɤɢɯ ɩɪɨɢɡɜɨɞɧɵɯ ɰɢɤɥɟɧɚ ɞɥɹ ɢɧɢɰɢɢɪɨɜɚɧɢɹ ɢɟɪɚɪɯɢɱɟɫɤɨɣ ɫɛɨɪɤɢ ɪɚɡɥɢɱɧɵɯ ɦɨɥɟɤɭɥ, ɫɨɞɟɪɠɚɳɢɯ ɜ ɫɜɨɟɦ ɫɨɫɬɚɜɟ ɢɦɢɞɧɵɟ ɢɥɢ ɮɨɫɮɚɬɧɵɟ ɝɪɭɩɩɵ, ɩɪɟɠɞɟ ɜɫɟɝɨ, ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹ ɢ ɢɯ ɩɪɨɢɡɜɨɞɧɵɟ (ɧɭɤɥɟɨɬɢɞɵ, ɧɭɤɥɟɢɧɨɜɵɟ ɤɢɫɥɨɬɵ ɢ ɢɯ ɮɪɚɝɦɟɧɬɵ). ɉɪɢ ɷɬɨɦ ɷɮɮɟɤɬɢɜɧɨɫɬɶ ɫɜɹɡɵɜɚɧɢɹ ɤɨɦɩɨɧɟɧɬɨɜ ɫɢɫɬɟɦɵ ɡɚɜɢɫɢɬ ɨɬ ɫɬɟɩɟɧɢ ɫɬɟɪɢɱɟɫɤɨɝɨ ɫɨɨɬɜɟɬɫɬɜɢɹ ɨɛɪɚɡɭɸɳɢɯ ɟɟ ɦɨɥɟɤɭɥ. ɇɚ ɛɢɫɰɢɤɥɢɱɟɫɤɢɯ ɬɟɦɩɥɚɬɚɯ ɪɨɫɬ 1 Ɋɚɛɨɬɚ ɜɵɩɨɥɧɟɧɚ ɩɪɢ ɮɢɧɚɧɫɨɜɨɣ ɩɨɞɞɟɪɠɤɟ ɊɎɎɂ (ɝɪɚɧɬ ʋ 05-0332195 ɢ ɝɪɚɧɬ ʋ 07-03-13519-ɨɮɢ_ɰ) ɢ ɈɏɇɆ ɊȺɇ (ʋ7) 596 Структура и динамика молекулярных систем, 2007 г., Выпуск №1 ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɨɣ ɫɬɪɭɤɬɭɪɵ ɩɪɨɬɟɤɚɟɬ ɩɨ «ɤɚɫɤɚɞɧɨɣ» ɫɯɟɦɟ: ɤɨɨɪɞɢɧɚɰɢɨɧɧɨɟ ɫɜɹɡɵɜɚɧɢɟ ɬɟɦɩɥɚɬɚ ɫ ɫɭɛɫɬɪɚɬɨɦ (ɧɚɩɪɢɦɟɪ, ɩɪɨɢɡɜɨɥɶɧɵɦ ɧɭɤɥɟɨɬɢɞɨɦ) (ɪɢɫ.2ɚ) ɜɡɚɢɦɨɞɟɣɫɬɜɢɟ ɫɜɹɡɚɧɧɨɝɨ ɫ ɬɟɦɩɥɚɬɨɦ ɧɭɤɥɟɨɬɢɞɚ ɢ ɟɝɨ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɩɚɪɬɧɟɪɚ ɢɡ ɪɚɫɬɜɨɪɚ ɡɚ ɫɱɟɬ ɨɛɪɚɡɨɜɚɧɢɹ ɜɨɞɨɪɨɞɧɵɯ ɫɜɹɡɟɣ ɦɟɠɞɭ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹɦɢ ɢ ɫɬɟɪɢɱɟɫɤɢɨɛɭɫɥɨɜɥɟɧɧɚɹ ɨɪɢɟɧɬɚɰɢɹ ɤɨɧɰɟɜɨɣ ɮɭɧɤɰɢɨɧɚɥɶɧɨɣ ɝɪɭɩɩɵ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɧɚ ɬɟɦɩɥɚɬ (ɪɢɫ.2.ɛ) ɤɨɨɪɞɢɧɚɰɢɨɧɧɨɟ ɫɜɹɡɵɜɚɧɢɟ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɫ ɬɟɦɩɥɚɬɨɦ (ɪɢɫ.2.ɜ) ɪɚɫɩɨɡɧɚɜɚɧɢɟ ɫɨɨɬɜɟɬɫɬɜɭɸɳɢɯ ɧɭɤɥɟɨɬɢɞɨɜ ɢɡ ɪɚɫɬɜɨɪɚ ɩɭɬɟɦ ɨɛɪɚɡɨɜɚɧɢɹ ɜɨɞɨɪɨɞɧɵɯ ɫɜɹɡɟɣ ɫ ɡɚɤɪɟɩɥɟɧɧɨɣ ɧɚ ɬɟɦɩɥɚɬɟ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɣ ɩɚɪɨɣ ɧɭɤɥɟɨɬɢɞɨɜ (ɪɢɫ.2.ɝ). ȼ ɪɟɡɭɥɶɬɚɬɟ ɮɨɪɦɢɪɭɸɬɫɹ ɝɟɬɟɪɨɝɟɧɧɵɟ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɟ ɛɢɫɥɨɢ, ɜ ɤɨɬɨɪɵɯ ɤɚɠɞɵɣ ɫɦɟɲɚɧɧɵɣ ɦɨɧɨɫɥɨɣ ɫɨɞɟɪɠɢɬ ɪɚɜɧɨɟ ɱɢɫɥɨ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɣ [1]. ɋɨɝɥɚɫɧɨ ɩɪɢɜɟɞɟɧɧɨɦɭ ɜɵɲɟ ɦɟɯɚɧɢɡɦɭ, ɮɨɪɦɢɪɨɜɚɧɢɟ ɝɨɦɨɝɟɧɧɵɯ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɛɢɫɥɨɟɜ, ɜ ɤɨɬɨɪɵɯ ɤɚɠɞɵɣ ɦɨɧɨɫɥɨɣ ɫɨɞɟɪɠɢɬ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹ ɬɨɥɶɤɨ ɨɞɧɨɝɨ ɬɢɩɚ, ɧɟɜɨɡɦɨɠɧɨ. Ⱦɥɹ ɪɟɚɥɢɡɚɰɢɢ ɫɚɦɨɫɛɨɪɤɢ ɝɨɦɨɝɟɧɧɵɯ ɛɢɫɥɨɟɜ ɛɵɥɢ ɫɮɨɪɦɢɪɨɜɚɧɵ ɬɟɦɩɥɚɬɵ ɢɡ ɦɨɧɨɰɢɤɥɢɱɟɫɤɨɝɨ ɩɪɨɢɡɜɨɞɧɨɝɨ (Zn2+-Ⱦɐɐ). ɉɨɫɥɟɞɨɜɚɬɟɥɶɧɚɹ ɚɞɊɢɫ.2. «Ʉɚɫɤɚɞɧɚɹ» ɫɯɟɦɚ ɪɨɫɬɚ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɨɣ ɫɬɪɭɤɬɭɪɵ. ɫɨɪɛɰɢɹ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɬɢɞɨɜ (ɭɪɢɞɢɧɮɨɫɮɚɬɨɜ 5-ɍɏɎ ɢ ɚɞɟɧɨɡɢɧɮɨɫɮɚɬɨɜ 5-ȺɏɎ, ɝɞɟ ɏ ɱɢɫɥɨ ɮɨɫɮɚɬɧɵɯ ɝɪɭɩɩ) ɧɚ ɋȺɆ-ɩɥɟɧɤɚɯ ɅȻ ɢɡ Zn2+-Ⱦɐɐ ɛɵɥɚ ɢɫɫɥɟɞɨɜɚɧɚ ɦɟɬɨɞɨɦ ɩɨɜɟɪɯɧɨɫɬɧɨɝɨ ɩɥɚɡɦɨɧɧɨɝɨ ɪɟɡɨɧɚɧɫɚ (ɉɉɊ). Ʉɢɧɟɬɢɤɚ ɫɜɹɡɵɜɚɧɢɹ ɧɭɤɥɟɨɡɢɞ ɦɨɧɨɮɨɫɮɚɬɨɜ ɧɚ ɬɚɤɨɣ ɩɨɜɟɪɯɧɨɫɬɢ ɢ ɫɨɨɬɜɟɬɫɬɜɭɸɳɢɟ ɉɉɊ-ɨɬɤɥɢɤɢ ɫɯɨɞɧɵ ɞɥɹ ɨɛɟɢɯ ɫɬɚɞɢɣ ɚɞɫɨɪɛɰɢɢ ɢ ɧɟ ɡɚɜɢɫɹɬ ɨɬ ɩɨɪɹɞɤɚ ɜɜɟɞɟɧɢɹ ɧɭɤɥɟɨɬɢɞɨɜ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ. Ȼɵɥ ɩɪɟɞɥɨɠɟɧ ɦɟɯɚɧɢɡɦ ɫɬɚɞɢɣɧɨɣ ɚɞɫɨɪɛɰɢɢ ɧɭɤɥɟɨɬɢɞɨɜ ɧɚ ɦɨɧɨɰɢɤɥɢɱɟɫɤɨɦ ɬɟɦɩɥɚɬɟ ɩɨ ɫɬɭɩɟɧɱɚɬɨɦɭ ɦɨɧɨɜɚɥɟɧɬɧɨɦɭ ɦɟɯɚɧɢɡɦɭ. Ɉɞɢɧ ɢɡ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɩɚɪɬɧɟɪɨɜ ɤɨɨɪɞɢɧɢɪɭɟɬɫɹ ɧɚ Zn2+-Ⱦɐɐ ɡɚ ɫɱɟɬ ɫɜɹɡɵɜɚɧɢɹ ɤɨɧɰɟɜɨɣ ɮɨɫɮɚɬɧɨɣ ɝɪɭɩɩɵ, ɮɨɪɦɢɪɭɹ ɬɟɦɩɥɚɬɧɭɸ ɦɚɬɪɢɰɭ ɫ ɨɫɧɨɜɚɧɢɹɦɢ, ɷɤɫɩɨɧɢɪɨɜɚɧɧɵɦɢ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ. ɉɨɫɥɟɞɭɸɳɟɟ ɪɚɫɩɨɡɧɚɜɚɧɢɟ ɨɫɭɳɟɫɬɜɥɹɟɬɫɹ ɡɚ ɫɱɟɬ ɜɵɫɨɤɨɫɩɟɰɢɮɢɱɟɫɤɨɝɨ ɨɛɪɚɡɨɜɚɧɢɹ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɩɚɪ Раздел 2 597 ɦɟɠɞɭ ɨɫɧɨɜɚɧɢɹɦɢ ɫɨɨɬɜɟɬɫɬɜɭɸɳɢɯ ɧɭɤɥɟɨɬɢɞɨɜ ɬɟɦɩɥɚɬɧɨɣ ɦɚɬɪɢɰɵ ɢ ɧɭɤɥɟɨɬɢɞɨɜ ɢɡ ɤɨɧɬɚɤɬɧɨɝɨ ɪɚɫɬɜɨɪɚ. ȼ ɪɟɡɭɥɶɬɚɬɟ ɮɨɪɦɢɪɭɸɬɫɹ ɝɨɦɨɝɟɧɧɵɟ ɛɢɫɥɨɢ ɧɭɤɥɟɨɬɢɞɨɜ, ɜ ɤɨɬɨɪɵɯ ɤɚɠɞɵɣ ɦɨɧɨɫɥɨɣ ɫɨɞɟɪɠɢɬ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɹ ɬɨɥɶɤɨ ɨɞɧɨɝɨ ɬɢɩɚ (ɪɢɫ. 3) [2]. Ɍɚɤɢɦ ɨɛɪɚɡɨɦ, ɦɟɯɚɧɢɡɦ ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɢ ɩɥɚɧɚɪɧɨɣ ɫɢɫɬɟɦɵ («ɞɜɨɣɧɨɣ ɩɥɨɫɤɨɫɬɢ» ɢɡ ɧɭɤɥɟɨɬɢɞɨɜ) ɧɚɩɪɹɦɭɸ ɨɩɪɟɞɟɥɹɟɬɫɹ ɦɨɥɟɤɭɥɹɪɧɨɣ ɝɟɨɦɟɬɪɢɟɣ ɬɟɦɩɥɚɬɚ ɢ ɫɬɪɨɟɧɢɟɦ ɪɚɫɩɨɡɧɚɸɳɟɝɨ ɦɚɤɪɨɰɢɤɥɚ-ɪɟɰɟɩɬɨɪɚ. Ⱦɥɹ ɜɵɹɜɥɟɧɢɹ ɡɚɤɨɧɨɦɟɪɧɨɫɬɟɣ ɩɪɢ ɮɨɪɦɢɪɨɜɚɧɢɢ ɝɟɬɟɪɨɝɟɧɧɵɯ ɛɢɫɥɨɟɜ, ɛɵɥɚ ɨɫɭɳɟɫɬɜɥɟɧɚ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɚɹ ɫɚɦɨɫɛɨɪɊɢɫ.3 ɋɯɟɦɚ ɫɬɪɭɤɬɭɪɵ ɝɨɦɨ- ɤɚ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɬɢɞɨɜ ɫɟɦɟɣɝɟɧɧɵɯ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɛɢ- ɫɬɜɚ ɝɭɚɧɢɧɚ ɢ ɰɢɬɨɡɢɧɚ (5-ȽɌɎ ɢ 5ɫɥɨɟɜ ɧɭɤɥɟɨɬɢɞɨɜ, ɮɨɪɦɢɪɭɸɳɢɯɫɹ ɜ ɪɟɡɭɥɶɬɚɬɟ ɫɬɚɞɢɣɧɨɣ ɐɌɎ). Ʉɢɧɟɬɢɱɟɫɤɚɹ ɤɪɢɜɚɹ ɫɬɚɞɢɣɧɨɣ ɚɞɚɞɫɨɪɛɰɢɢ ɧɚ ɦɨɧɨɰɢɤɥɢɱɟɫɤɨɦ ɫɨɪɛɰɢɢ ɞɥɹ ɷɬɢɯ ɧɭɤɥɟɨɬɢɞɨɜ ɩɪɟɞɫɬɚɜɥɟɬɟɦɩɥɚɬɟ. ɧɚ ɧɚ ɪɢɫ.4. ɋɨɝɥɚɫɧɨ ɤɪɢɜɨɣ, ɚɧɚɥɨɝɢɱɧɨɣ ɩɨ ɮɨɪɦɟ ɤɢɧɟɬɢɱɟɫɤɨɣ ɤɪɢɜɨɣ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɝɨ ɫɜɹɡɵɜɚɧɢɹ 5-ȺɌɎ ɢ 5-ɍɌɎ, ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɹ ɝɭɚɧɢɞɢɧɨɜɵɯ ɢ ɰɢɬɨɡɢɧɨɜɵɯ ɧɭɤɥɟɨɬɢɞɨɜ ɩɪɢɜɨɞɢɬ ɤ ɨɛɪɚɡɨɜɚɧɢɸ ɝɟɬɟɪɨɝɟɧɧɨɝɨ ɛɢɫɥɨɹ ɩɨ ɤɨɨɩɟɪɚɬɢɜɧɨɦɭ ɦɟɯɚɧɢɡɦɭ. Ʉɪɨɦɟ ɬɨɝɨ, ɭɫɩɟɲɧɨɟ ɮɨɪɦɢɪɨɜɚɧɢɟ ɬɚɤɨɣ ɫɢɫɬɟɦɵ ɜɨɡɦɨɠɧɨ ɬɨɥɶɤɨ ɩɪɢ ɢɧɢɰɢɢɪɨɜɚɧɢɢ ɚɞɫɨɪɛɰɢɢ ɰɢɬɢɞɢɧɨɜɵɦ ɧɭɤɥɟɨɬɢɞɨɦ. ɇɚɱɚɥɶɧɚɹ ɚɞɫɨɪɛɰɢɹ ɝɭɚɧɢɞɢɧɨɜɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɨɤɚɡɚɥɚɫɶ ɧɟɷɮɮɟɤɬɢɜɧɨɣ, ɢ ɦɨɞɢɮɢɰɢɪɨɜɚɧɧɚɹ ɷɬɢɦ ɧɭɤɥɟɨɬɢɞɨɦ ɩɨɜɟɪɯɧɨɫɬɶ ɨɛɥɚɞɚɥɚ ɧɢɡɤɨɣ ɚɤɬɢɜɧɨɫɬɶɸ ɩɨ ɨɬɧɨɲɟɧɢɸ ɤ ɤɨɦɩɥɟɦɟɧɊɢɫ.4 ɋɟɧɫɨɝɪɚɦɦɚ ɉɉɊ ɞɥɹ ɬɟɦɩɥɚɬɨɜ ɬɚɪɧɨɦɭ ɩɚɪɬɧɟɪɭ. ȼɟɪɨɹɬɧɨɣ ɩɪɢZn2+-Ȼɐ ɩɪɢ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɦ ɞɨɛɚɜɥɟ- ɱɢɧɨɣ ɷɬɨɝɨ ɷɮɮɟɤɬɚ ɦɨɠɟɬ ɹɜɥɹɬɶɫɹ ɧɢɢ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ ɰɢɬɢɞɢɧɮɨɫɮɚɬɚ/ɝɭɚɧɨɡɢɧɮɨɫɮɚɬɚ /ɰɢɬɢɞɢɧɮɨɫɮɚɬɚ ɧɢɡɤɚɹ ɫɬɚɛɢɥɶɧɨɫɬɶ ɝɭɚɧɢɞɢɧɨɜɵɯ (ɩɨɥɧɚɹ ɬɪɟɯɫɬɚɞɢɣɧɚɹ ɤɨɨɩɟɪɚɬɢɜɧɚɹ ɧɭɤɥɟɨɬɢɞɨɜ ɜ ɜɨɞɧɵɯ ɪɚɫɬɜɨɪɚɯ ɩɪɢ ɫɛɨɪɤɚ); ɤɨɧɰɟɧɬɪɚɰɢɢ ɧɭɤɥɟɨɬɢɞɨɜ 0.1 ɞɚɧɧɵɯ ɪɇ, ɩɪɢɜɨɞɹɳɚɹ ɤ ɛɵɫɬɪɨɦɭ ɝ/ɥ, ɪɇ 7.55. ɝɢɞɪɨɥɢɡɭ ɫɜɹɡɚɧɧɨɝɨ ɫ ɦɚɬɪɢɰɟɣ ȽɌɎ. ȼ ɪɟɡɭɥɶɬɚɬɟ ɝɢɞɪɨɥɢɬɢɱɟɫɤɨɝɨ ɪɚɫɳɟɩɥɟɧɢɹ ɧɭɤɥɟɨɨɫɧɨɜɚɧɢɟ ɭɯɨɞɢɬ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ, ɨɫɬɚɜɥɹɹ ɧɚ ɩɨɜɟɪɯɧɨɫɬɢ ɪɢɛɨɡɨ-5ɮɨɫɮɚɬ, ɧɟɫɩɨɫɨɛɧɵɣ ɤ ɚɞɫɨɪɛɰɢɢ ɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ. 598 Структура и динамика молекулярных систем, 2007 г., Выпуск №1 Ⱦɥɹ ɩɨɞɬɜɟɪɠɞɟɧɢɹ ɦɟɯɚɧɢɡɦɚ ɤɨɨɩɟɪɚɬɢɜɧɨɣ ɫɚɦɨɫɛɨɪɤɢ, ɜ ɱɚɫɬɧɨɫɬɢ, ɨɛɪɚɡɨɜɚɧɢɹ ɫɬɪɭɤɬɭɪɵ ɫ ɭɱɚɫɬɢɟɦ ɫɩɟɰɢɮɢɱɟɫɤɢɯ ɜɨɞɨɪɨɞɧɵɯ ɜɡɚɢɦɨɞɟɣɫɬɜɢɣ ɜ ɜɨɞɧɨɣ ɫɪɟɞɟ, ɛɵɥɢ ɩɪɨɜɟɞɟɧɵ ɢɫɫɥɟɞɨɜɚɧɢɹ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɣ ɚɞɫɨɪɛɰɢɢ ɧɟɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɫɨɱɟɬɚɧɢɣ ɧɭɤɥɟɨɬɢɞɨɜ. Ʉɚɤ ɜɢɞɧɨ ɢɡ ɪɢɫ.5, ɛɢɫɰɢɤɥɢɱɟɫɤɢɣ ɬɟɦɩɥɚɬ ɫ ɩɪɟɞɜɚɪɢɬɟɥɶɧɨ ɢɦɦɨɛɢɥɢɡɨɜɚɧɧɵɦ ɰɢɬɢɞɢɧɨɜɵɦ ɧɭɤɥɟɨɬɢɞɨɦ (ɐɌɎ) ɩɢɪɢɦɢɞɢɧɨɜɨɝɨ ɪɹɞɚ ɧɟ ɫɩɨɫɨɛɟɧ ɤ ɫɩɟɰɢɮɢɱɟɫɤɨɣ ɚɞɫɨɪɛɰɢɢ ɧɟɤɨɦɩɥɟɦɟɧɬɚɪɧɨɝɨ ɧɭɤɥɟɨɬɢɞɚ ɩɭɪɢɧɨɜɨɝɨ ɪɹɞɚ (ȺɌɎ). ȼ ɨɬɥɢɱɢɟ ɨɬ ɤɨɨɩɟɪɚɬɢɜɧɨɝɨ ɪɚɫɩɨɡɧɚɜɚɧɢɹ ɫɢɫɬɟɦɵ ɐɌɎ/ȽɌɎ ɫ ɞɜɭɤɪɚɬɧɵɦ ɜɬɨɪɢɱɧɵɦ ɨɬɤɥɢɤɨɦ ɉɉɊ ɨɬɧɨɫɢɬɟɥɶɧɨ ɨɬɤɥɢɤɚ ɧɚ ɩɟɪɜɨɣ ɫɬɚɞɢɢ (ɫɦ. ɪɢɫ.4), ɜ ɞɚɧɧɨɦ ɫɥɭɱɚɟ ɧɚɛɥɸɞɚɟɬɫɹ ɧɟɫɩɟɰɢɮɢɱɟɫɤɚɹ ɚɞɫɨɪɛɰɢɹ ɫ ɨɬɤɥɢɤɨɦ ɜ ɞɜɚ ɪɚɡɚ ɦɟɧɶɲɢɦ, ɱɟɦ ɨɬɤɥɢɤ ɩɪɢ ɩɟɪɜɢɱɧɨɣ ɚɞɫɨɪɛɰɢɢ ɐɌɎ. Ȼɨɥɟɟ ɬɨɝɨ, ɧɟɫɩɟɰɢɮɢɱɟɫɤɢ ɚɞɫɨɪɛɢɪɨɜɚɧɧɵɣ ȺɌɎ ɩɨɥɧɨɫɬɶɸ ɭɞɚɥɹɟɬɫɹ ɫ ɩɨɜɟɪɯɧɨɫɬɢ ɐɌɎɬɟɦɩɥɚɬ ɩɪɢ ɩɪɨɦɵɜɚɧɢɢ ɉɉɊ-ɹɱɟɣɤɢ ɱɢɫɬɨɣ ɜɨɞɨɣ. Ɍɚɤɢɦ ɨɛɪɚɡɨɦ, ɦɟɯɚɧɢɡɦ ɤɨɨɩɟɪɚɬɢɜɧɨɣ ɫɛɨɪɤɢ ɫɬɪɨɝɨ ɫɩɟɰɢɮɢɱɟɧ, ɚ ɫɚɦɨɨɪɝɚɧɢɡɚɰɢɹ ɩɥɚɧɚɪɧɨɣ ɛɢɫɥɨɣɧɨɣ ɫɬɪɭɤɬɭɪɵ ɧɚ ɦɚɤɪɨɰɢɤɥɢɱɟɫɤɢɯ ɬɟɦɩɥɚɬɚɯ ɜɨɡɦɨɠɧɚ ɬɨɥɶɤɨ ɢɡ ɤɨɦɩɥɟɦɟɧɬɚɪɧɵɯ ɧɭɤɥɟɨɬɢɞɨɜ. ɋɥɟɞɭɟɬ ɨɬɦɟɬɢɬɶ, ɱɬɨ ɢɫɫɥɟɞɨɜɚɧɧɵɣ ɩɪɨɰɟɫɫ ɫɚɦɨɨɪɊɢɫ.5 ɋɟɧɫɨɝɪɚɦɦɚ ɉɉɊ ɞɥɹ ɬɟɦɩɥɚɬɨɜ Zn2+ɝɚɧɢɡɚɰɢɢ ɹɜɥɹɟɬɫɹ ɨɞɧɢɦ ɢɡ Ȼɐ ɩɪɢ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɦ ɞɨɛɚɜɥɟɧɢɢ ɜ ɤɨɧɬɚɤɬɧɵɣ ɪɚɫɬɜɨɪ ɰɢɬɢɞɢɧɮɨɫɮɚɬɚ ɢ ɚɞɟɧɨ- ɪɟɞɱɚɣɲɢɯ ɩɪɢɦɟɪɨɜ ɤɨɨɩɟɪɚɡɢɧɮɨɫɮɚɬɚ ɫ ɩɨɫɥɟɞɭɸɳɢɦ ɩɪɨɦɵɜɚɧɢɟɦ ɬɢɜɧɨɣ ɫɛɨɪɤɢ ɢɫɤɭɫɫɬɜɟɧɧɵɯ ɤɨɧɬɚɤɬɧɨɣ ɹɱɟɣɤɢ ɱɢɫɬɨɣ ɜɨɞɨɣ (ɩɨɥɧɚɹ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɵɯ ɫɢɫɬɟɦ ɧɚ ɬɪɟɯɫɬɚɞɢɣɧɚɹ ɤɨɨɩɟɪɚɬɢɜɧɚɹ ɫɛɨɪɤɚ); ɤɨɧɰɟɧɬɜɟɪɞɵɯ ɩɨɜɟɪɯɧɨɫɬɹɯ. Ʉɪɨɦɟ ɬɪɚɰɢɢ ɧɭɤɥɟɨɬɢɞɨɜ 0.1 ɝ/ɥ, ɪɇ 7.55. ɬɨɝɨ, ɩɪɟɞɥɨɠɟɧɧɵɣ ɩɨɞɯɨɞ ɩɨɡɜɨɥɹɟɬ ɩɪɟɨɞɨɥɟɜɚɬɶ ɨɝɪɚɧɢɱɟɧɢɟ, ɧɚɥɚɝɚɟɦɨɟ ɧɚ ɫɚɦɭ ɜɨɡɦɨɠɧɨɫɬɶ ɫɢɧɬɟɡɚ ɫɭɩɪɚɦɨɥɟɤɭɥɹɪɧɵɯ ɫɬɪɭɤɬɭɪ ɩɨɫɪɟɞɫɬɜɨɦ ɜɨɞɨɪɨɞɧɵɯ ɫɜɹɡɟɣ ɜ ɜɨɞɧɨɣ ɫɪɟɞɟ, ɡɚ ɫɱɟɬ ɫɩɟɰɢɮɢɱɟɫɤɨɣ ɝɟɨɦɟɬɪɢɢ ɬɟɦɩɥɚɬɚ ɢ ɢɫɩɨɥɶɡɨɜɚɧɢɹ ɫɨɜɨɤɭɩɧɨɫɬɢ ɮɢɡɢɤɨ-ɯɢɦɢɱɟɫɤɢɯ ɫɜɨɣɫɬɜ ɞɜɭɦɟɪɧɵɯ ɫɢɫɬɟɦ ɧɚ ɝɪɚɧɢɰɟ ɪɚɡɞɟɥɚ ɮɚɡ. Ʌɢɬɟɪɚɬɭɪɚ: 1. Turygin D.S., Subat M., Raitman O.A., Arslanov V.V., K nig B., Kalinina M.A. // Cooperative Self-Assembly of Adenosine and Uridine Nucleotides on a 2D Synthetic Template // Angewandte Chemie Int. Ed. 2006, 45, 5340 5344. 2. D.S. Turygin, M. Subat, O.A. Raitman, S.L. Selector, V.V. Arslanov, B. König, M.A. Kalinina // Two-dimensional Arrays of Amphiphilic Zn2+-cyclens for Guided Molecular Recognition at Interfaces // Langmuir, 2007, 23, 2517-2524.
