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. 2012. 2. . 15–20. 676.014; 577.152.37 © * . , . , ( . , . ) . , 17, , 163002 ( . , ), e-mail: biotech@narfu.ru , - . . , S1 : , . , , . , [1– : ; - , 3]. ; , ( ) [3]. , . , . ( ) . - , . – [4]. , , - – , , e-mail: biotech@narfu.ru [5–8]. , - – , e-mail: biotech@narfu.ru , , – . , e-mail: biotech@narfu.ru [9–11], – , e-mail: biotech@narfu.ru * , . . - 16 . , . , . . , . , , , . , , [5–8]. , , - , [12]. 20–40% [13–15]. , . – , , , . . - , . , Fiber Care D Fiber Care D: ( – 273 , . Novozymes A/S ( ). ) – 9800 ECU/ , pH – 6–9, – 40– 60 °C. 3%. 50 °C 3 20 48 . . . . , , - , . - . . , – 150–250 2–3%. 59% . 5–50 . , - . . -2 [16]. -2 , , 5 . . . . . , S1 - . , , . - 17 … : , NaOH, , - . . - [12–15]. , . , . 3, 6, 24 48 1. S1, . , - . 24 . , , S1 , 48 . , , , , 3–6 , , - [3, 17]. , , . - . , , . , [3, 18], [3, 19]. . 59% ; , » »; « » « (« » [3, 19]. , : ; , . » « « 2. - ») S1 S2, , « »( S1. . 1. 1– 2– ; :. ; S2 . 2 ). - 18 . . 2. « , . , » . , ( ) . « » , ( ) « » , , ( . 2 ). - , , - , . , . « S1 , », . , . « » , . S1 , - . , , S2. 3 - , . . - , 3 ( . 1). - 24–48 . ( . 2). , . , 2 ( 25 . 2). . 19 ° , 1. , , , * , * - 3 3 24 48 . 17 15 16 17 500 6 4 5 7 700 13 10 11 15 - 19 … 2. * , 3 3 24 48 - , ,° , 500 15 8 8 12 25 19 19 20 700 33 18 18 24 * . 48 , , - , - , - . , . . 1. , , , . 2. 59% , S1 . 3. , . 1. 2. 3. ., . ( : 3., ., ., ., 4. 5. 6. 7. , ., ., , . ): ., 2004. ., . . ., ., ., 1988. 512 . ., ., , 1972. 509 . ., ., . . ., ., . // . . . 2006. . . 15–17. Bajpaj P. Application of enzymes in the pulp and paper industry // Biotechnology Progress. 1999. N15. Pp. 147–157. Aehle W. Enzymes in Industry. Weinheim, 2004. 484 . . // ., : 8. 9. 10. 11. 12. 13. . . .- . . , 2006. . 31–34. ., ., . // , . , 2009. . 45–48. Yllner S., Enstrom B. Studies of the adsorbtion of xylan on celluloses fibres during the sulphate cook. Part I // Svensk papperstidning. 1956. Vol. 59, N6. Pp. 229–232. Yllner S., Enstrom B. Studies of the adsorbtion of xylan on celluloses fibres during the sulphate cook. Part II // Svensk papperstidning. 1957. Vol. 60, N15. Pp. 549–554. ., . . ., 1972. 440 . ., ., ., . // : . . . . . 2010. . 83. . 101–103. ., . ( ) // . 2011. 3. . 15–32. 20 . , . , . , . 14. Engström A.-C., Ek M., Henriksson G. Improved accessibility and reactivity of dissolving pulp for the viscose process: pretreatment with monocomponent endoglucanase // Biomacromolecules. 2006. Vol. 7, N6. p. 2027–2031. 15. Kopcke V., Ibarra D., Ek M. Increasing accessibility and reactivity of paper grade pulp by enzymatic treatment for use as dissolving pulp // Nordic Pulp and Paper Research Journal. 2008. Vol. 23, N4. p. 363–368. 16. ., ., ., ., ., ., . : . ., 2006. 230 . 17. . : . . ., 1988. 440 . 18. ., ., . . ., 1978. 368 . 19. Paul Ander. Dislocations and balloon swelling in spruce kraft pulp fibres – Effect of cellulases, xylanase and laccase/HBT // Biotechnology in the Pulp and Paper Industry, L. Viikari, R. Lantto, editors. Elsevier Science. 2002. Pp. 49–59. 28 2012 . Novozhilov E.V.*, Chukhchin D.G., Terentyev K.Y., Hadyko I.A. CHANGES OF CELL WALL STRUCTURE AND FIBER PROPERTIES OF BLEACHED KRAFT HARDWOOD PULP BY ENZYMATIC ACTION Northern (Arctic) Federal University named after M.V. Lomonosov, Severnaya Dvina Emb. 17, Arkhangelsk, 163002 (Russia), e-mail: biotech@narfu.ru It is established that there are structure’s changes of a cellular wall of cellulose fibres after cellulase treatment. It is shown an applicability of the developed techniques for determination of fiber’s width and control of cellulose fibres swelling. A variety of swelling forms is defined by surface’s destruction degree of bleached hardwood kraft cellulose fibres, a major factor is the condition of external layer S1 of a cellular wall. Keywords: bleached hardwood kraft cellulose, fiber’s width, fibres swelling, cellulase. References 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Fengel D., Vegener G. Drevesina (khimiia, ul'trastruktura, reaktsii) [Wood (chemistry, ultrastructure, reactions)]. Moscow., 1988. 512 p. (in Russ.). Tekhnologiia tselliulozno-bumazhnogo proizvodstva [Technology Pulp and Paper Production]. St. Petersburg, 2004, vol. 1–3. (in Russ.). Beinart I.I., Vedernikov N.A., Gromov V.S., Zakis G.F., Kal'nina V.K., Katkevich R.G., Katkevich Iu.Iu., Kreitsberg Z.N., Miliutina S.V., Mozheiko L.N., Murashchenko N.F., Reizin' R.E., Sergeeva V.N., Erin'sh P.P. Kletochnaia stenka drevesiny i ee izmeneniia pri khimicheskom vozdeistvii [The cell wall of wood and its changes during chemical exposure]. 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Svensk papperstidning, 1957, vol. 60, no. 15, pp. 549–554. Sharkov V.I., Kuibina N.I. Khimiia gemitselliuloz [Chemistry of hemicellulose]. Moscow, 1972, 440 p. (in Russ.). Novozhilov E.V., Terent'ev K.Iu., Poshina D.N., Chertovskaia V.P. Nauka Severnomu regionu: sb. nauch. trudov S(A)FU [Science North Region: collection of proceedings]. Arkhangel'sk, 2010, no. 83, pp. 101–103. (in Russ.). Novozhilov E.V., Poshina D.N. Khimiia rastitel'nogo syr'ia, 2011, no. 3, pp. 15–32. (in Russ.). Engström A.-C., Ek M., Henriksson G. Biomacromolecules, 2006, vol. 7, no. 6, pp. 2027–2031. Kopcke V., Ibarra D., Ek M. Nordic Pulp and Paper Research Journal, 2008, vol. 23, no. 4, pp. 363–368. Dubovyi V.K., Gur'ev A.V., Kazakov Ia.V., Komarov V.I., Konovalova G.N., Smolin A.S., Khovanskii V.V. Laboratornyi praktikum po tekhnologii bumagi i kartona: uchebnoe posobie [Laboratory workshop on the technology of paper and paperboard]. St. Peterburg, 2006, 230 p. (in Russ.). Fliate D.M. Tekhnologiia bumagi. [Paper technology]. Moscow, 1988, 440 p. (in Russ.). Nikitin V.M., Obolenskaia A.V., Shchegolev V.P. Khimiia drevesiny i tselliulozy. [Chemistry of wood and cellulose] M., 1978. 368 s. (in Russ.). Paul Ander. Biotechnology in the Pulp and Paper Industry. ed. L. Viikari, R. Lantto. Elsevier Science, 2002, pp. 49–59. Received Marh 28, 2011 * Corresponding author.
