Section II
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Biological Risk Assessment
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Biological Risk Assessment
HAZARDOUS CHARACTERISTICS OF AN AGENT
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!
TABLE 1
CLASSIFICATION OF INFECTIOUS MICROORGANISMS BY RISK GROUP
RISK GROUP
CLASSIFICATION
NIH GUIDELINES FOR RESEARCH
INVOLVING RECOMBINANT DNA
2
MOLECULES 2002
WORLD HEALTH ORGANIZATION
LABORATORY BIOSAFETY MANUAL
3RD EDITION 2004 *
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Biological Risk Assessment
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Biological Risk Assessment
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=KS>LPB5'J,)'"22$1$,*"#'2+1"$#!'!++'K00+*2$D'J5'
'
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.3")".1+)$!1$.!',-'C+*+1$."##8'/,2$-$+2'"C+*1!'$*%,#%+'.,*!$2+)"1$,*',-'13+'!"/+'-".1,)!'
(!+2'$*')$!:'"!!+!!/+*1',-'13+'9$#2@180+',)C"*$!/5'L1'$!'0")1$.(#")#8'$/0,)1"*1'1,'"22)+!!'
13+'0,!!$7$#$18'13"1'13+'C+*+1$.'/,2$-$."1$,*'.,(#2'$*.)+"!+'"*'"C+*1R!'0"13,C+*$.$18',)'
"--+.1'$1!'!(!.+01$7$#$18'1,'"*1$7$,1$.!',)',13+)'+--+.1$%+'1)+"1/+*1!5'63+')$!:'"!!+!!/+*1'
."*'7+'2$--$.(#1',)'$*.,/0#+1+&'7+."(!+'$/0,)1"*1'$*-,)/"1$,*'/"8'*,1'7+'"%"$#"7#+'-,)'"'
*+9#8'+*C$*++)+2'"C+*15'P+%+)"#'$*%+!1$C"1,)!'3"%+')+0,)1+2'13"1'13+8',7!+)%+2'
(*"*1$.$0"1+2'+*3"*.+2'%$)(#+*.+'$*')+.+*1'!1(2$+!'9$13'+*C$*++)+2'"C+*1!5U@<V'63+!+'
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%$)(#+*.+'C+*+!'/"8'#+"2'1,'$*.)+"!+2')$!:5'L1'"#!,'!(CC+!1!'13"1')$!:'"!!+!!/+*1'$!'"'
.,*1$*($*C'0),.+!!'13"1')+E($)+!'(02"1$*C'"!')+!+").3'0),C)+!!+!5'
'
63+'NIH Guidelines ")+'13+':+8')+-+)+*.+'$*'"!!+!!$*C')$!:'"*2'+!1"7#$!3$*C'"*'
"00),0)$"1+'7$,!"-+18'#+%+#'-,)'9,):'$*%,#%$*C')+.,/7$*"*1'QNK'/,#+.(#+!5V'63+'
0()0,!+',-'13+'NIH Guidelines'$!'1,'0),/,1+'13+'!"-+'.,*2(.1',-')+!+").3'$*%,#%$*C'
)+.,/7$*"*1'QNK5'63+'C($2+#$*+! !0+.$-8'"00),0)$"1+'0)".1$.+!'"*2'0),.+2()+!'-,)'
)+!+").3'$*%,#%$*C'.,*!1)(.1$*C'"*2'3"*2#$*C'7,13')+.,/7$*"*1'QNK'/,#+.(#+!'"*2'
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)+.,/7$*"*1'QNK'$*1,'W$!:'T),(0!'V&'X&'"*2'Y'"C+*1!&'"*2'+D0+)$/+*1!'$*'93$.3'13+'
QNK'-),/'W$!:'T),(0!'V&'X&'"*2'Y'"C+*1!'$!'.#,*+2'$*1,'*,*0"13,C+*$.'0),:")8,1$.',)'
#,9+)'+(:")8,1$.'3,!1@%+.1,)'!8!1+/!5';,/0#$"*.+'9$13'13+'NIH Guidelines'$!'/"*2"1,)8'
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Biological Risk Assessment
!"#$%&'()(*+,-.+!"/")!#01+2)(3+$*0"!+'(/*'*4*'$(/+0)5"+)6$7*"6+*0"/"+84'6"9'("/+)/+*0"+
&"/*+#4!!"(*+7!)#*'#"1++
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:0"+NIH Guidelines+;"!"+<'!/*+74&9'/0"6+'(+=>?@+)(6+)!"+!"5'/"6+$(+)(+$(8$'(8+&)/'/+'(+
!"/7$(/"+*$+/#'"(*'<'#+)(6+7$9'#3+6"5"9$7%"(*/1+:0"3+$4*9'("+*0"+!$9"/+)(6+!"/7$(/'&'9'*'"/+
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++
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"T7$/4!"+*$+&9$$6&$!("+7)*0$8"(/+<'()9+!49"+;$496+'(#946"+04%)(+#"99+9'("/1=?++
+
HAZARDOUS CHARACTERISTICS OF LABORATORY PROCEDURES
+
B(5"/*'8)*'$(/+$<+^.B/+0)5"+'6"(*'<'"6+<'5"+7!'(#'7)9+!$4*"/+$<+9)&$!)*$!3+*!)(/%'//'$(1+
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Biological Risk Assessment
!"##$%$&'()(*)$+&'($#,)(-&).*"(&)*#)(./'!0$!!$*')*#)$'#&%($*'1)2*.3)-/!)!-*4')(-/()(-&)
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+*'&1)
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G.*%&+".&!)(-/()$05/.()&'&.:,)(*)/)0$%.*6$/7)!"!5&'!$*')4$77)5.*+"%&)/&.*!*7!1)
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Biological Risk Assessment
POTENTIAL HAZARDS ASSOCIATED WITH WORK PRACTICES, SAFETY
EQUIPMENT AND FACILITY SAFEGUARDS
!
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!
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Biological Risk Assessment
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!
AN APPROACH TO ASSESS RISKS AND SELECT APPROPRIATE
SAFEGUARDS
!
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)$.+(5$('(/$.&'#&,2#5$()-2$.&'#'&5(+&"(+$-%8#
#
CONCLUSION
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