Epigentek Group Inc. 760 Parkside Avenue Brooklyn, NY 11226 United States of America Tel: 1-877-374-4368 Fax: 1-718-484-3956 E-mail: info@epigentek.com Web: www.epigentek.com Histone Modification Table Histone H1 H2A H2B H3 H4 Modified Site Modification Pattern Modifying Enzymes Proposed Function Ser27 Lys26 Lys 4 (S.cerevisiae) Lys5 (mammals) Lys7 (S. cerevisiae) Lys7 (S. cerevisiae) Ser 1 Thr119 (D. melanogaster) Ser122 (S. cerevisiae) Ser129 (S. cerevisiae) Ser139 (mammalian H2AX) Lys 119 (Mammals) Lys126 (S. cerevisiae) Lys9 Lys13 Lys5 Lys11 (S. cerevisiae) Lys12 (mammals) Lys15 (mammals) Lys16 (S. cerevisiae) Lys20 Ser10 (S. cerevisiae) Ser14 (vertebrates) Ser33 (D. melanogaster) Lys120 (mammals) Lys123 (S. cerevisiae) Lys6 or Lys7 (S. cerevisiae) Lys4 (S. cerevisiae) Lys9 Lys14 Phosphorylation Methylation Acetylation Acetylation Acetylation Acetylation Phosphorylation Phosphorylation Phosphorylation Phosphorylation Phosphorylation Ubiquitylation Sumoylation Biotinylation Biotinylation Acetylation Acetylation Acetylation Acetylation Acetylation Acetylation Phosphorylation Phosphorylation Phosphorylation Ubiquitylation Ubiquitylation Sumoylation Acetylation Acetylation Acetylation unknown Ezh2 Esa 1 Tip60, p300/CBP Hat1 Esa1 unknown, MSK1 NHK1 unknown Mec1, Tel1 ATR, ATM, DNA-PK Ring2 Ubc9 biotinidase biotinidase p300, ATF2 Gcn5 p300/CBP, ATF2 p300/CBP, ATF2 Gcn5, Esa1 p300 Ste20 Mst1, unknown TAF1 UbcH6 Rad6 Ubc9 Esa1, Hpa2 Gcn5, SRC-1, unknown unknown, Gcn5, PCAF, Esal, Tip60, SRC-1, Elp3, Hpa2, hTFIIIC90, TAF1, Sas2, Sas3, p300 transcriptional activation, chromatin decondensation transcriptional silencing Transcription activation transcriptional activation unknown transcriptional activation mitosis, chromatin assembly, transcriptional repression mitosis DNA repair DNA repair DNA repair spermatogenesis transcriptional repression unknown unknown transcriptional activation transcriptional activation transcriptional activation transcriptional activation transcriptional activation transcriptional activation apoptosis apoptosis, DNA repair transcriptional activation meiosis transcriptional activation, euchromatin transcriptional repression transcriptional activation transcriptional activation, histone deposition histone deposition, transcriptional activation, DNA repair , RNA polymerase II & III transcription, euchromatin Lys18 Lys23 Acetylation Acetylation Lys27 Lys56 (S. cerevisiae) Lys4 Acetylation Acetylation Methylation Arg8 Lys9 Methylation Methylation transcriptional activation, DNA repair and replication histone deposition , transcriptional activation, DNA repair transcriptional activation transcriptional activation, DNA repair permissive euchromatin (di-Me), transcriptional activation transcriptional repression transcriptional silencing (tri-Me), transcriptional repression, genomic imprinting, DNA methylation (tri-Me), transcriptional activation Arg17 Lys27 Lys36 Lys79 Methylation Methylation Methylation Methylation Gcn5, p300/CBP unknown, Gcn5, SAs3, p300/CBP Gcn5 Spt10 Set1 (S. cerevisiae), Set7/9 (vertebrates), MLL, ALL-1, PRMT5 Suv39h,Clr4, G9a, SETDB1, Dim-5 (N.crassa), Kryptonite (A. thaliana), Ash1 (D. melanogaster) CARM1 Ezh2, G9a Set2 Dot1 Thr3 Ser10 Phosphorylation Phosphorylation Haspin/Gsg2 Aurora-B kinase, MSK1, MSK2, IKK-α, Snf1 mitosis mitosis, meiosis, immediate-early gene activation, transcriptional activation Thr11 (mammals) Ser28 (mammals) Lys4 Lys9 Lys18 Lys5 Phosphorylation Phosphorylation Biotinylation Biotinylation Biotinylation Acetylation Dlk/Zip Aurora-B kinase, MSK1, MSK2 biotinidase biotinidase biotinidase Hat1, Esal, Tip60, ATF2, Hpa2, p300 mitosis mitosis, immediate-early activation gene expression gene expression gene expression histone deposition, transcriptional activation, DNA repair Lys8 Acetylation Gcn5, PCAF, Esal, Tip60, ATF2, Elp3, p300 transcriptional activation, DNA repair Lys12 Acetylation Hat1, Esal, Tip60, Hpa2, p300 histone deposition, telomeric silencing, transcriptional activation, DNA repair Lys16 Acetylation Gcn5, MOF (D. melanogaster), Esal, Tip60, ATF2, Sas2 transcriptional activation, DNA repair, euchromatin Lys91 (S. cerevisiae) Arg3 Arg3 Lys20 Acetylation Methylation Methylation Methylation Hat1/Hat2 PRMT1 PRMT5 PR-Set7, Suv4-20h, Ash1 (D. melanogaster), Set9 (S. pombe) chromatin assembly transcriptional activation transcriptional activation transcriptional silencing (mono-Me), heterochromatin (tri-Me), transcriptional activation, checkpoint response Lys59 Ser1 N-terminal tail (S. cerevisiae) Lys12 Methylation Phosphorylation Ubiquitylation Biotinylation unknown unknown, CK2 Ubc9 biotinidase transcriptional silencing mitosis, chromatin assembly, DNA repair transcriptional repression DNA damage response transcriptional activation transcriptional silencing, X inactivation (tri-Me) transcriptional activation (elongation) euchromatin, transcriptional activation (elongation), checkpoint response See related products at 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