» 616.127-005.4-036.11-02:611.83 03.03.04 – , 2016 , « ». , : , , « » , , : , « » , , , » « : » 8 03.18.03 2016 14.00 « : 220116, » , 83, e-mail: [email protected] « . ». «____» 2016 . , , . . , 1 , , . ., 2001; Jiménez B., 2002; Luhder F., 2009]. , . ( ) [ , [ 2010; Wood A., 2010]. . ., 2002; , . ., 2009; Lujan H. L., – , , [ , ., 2001; Herring N., 2012; Shanks J., 2013]. . ( ) [Wang L., 2011]. ) [Mosnaim A., 2013] [ . ., 2013; Gross G., 2010]. ( ) [Kimura O., 2006]. . , 2006; Jiménez B., 2002; Sámano C., 2006]. , [Nasu T., 2000; Arciszewski M. B., , . ( ), , , . 2 « »( ) « »( 2008355 12.03.2008 .) » ( « 20111041). : – , . : 1. , . 2. , . 3. , . – 59 45 . – , . , . , , . 3 , , . : , . , . , 1. , , . 2. , . 3. , , . 4. , . , . , , , , , , , , , , , , . , , . , . , , 4 [22], [11] – 90%. , [1–4], [15, 16, 17, 19, 21] 90 %. 8, 9, 12, 13] – [5, 6, 7, [10, 14, 15] – [18, 19, 20, 21] 95%. [22]. : « 2009, 2010, 2011); 17, 2009); 15- »( ( ( ( , 2011, 2013), 6( , 2011); 90« , , 2011); 6Radiation Health Risk Management» ( : ( . . ( , , 2014). , 2008, , 2009); , 2010); - ( , 2011); 22- » «Global Strategic Center for , 2011); ( , 2011); 8( , 2013); «9» , 2014); 100. 5 21 . , , – 4, – 3,1 51 – 10, – 7. . 111 , , ( (80 (24), 18 22 , 302 ), 222 ), 1 (5). , , 36 ). 98 45 59 . , , 30 , 68 ( , ). , . « » . 55-3 ». 24 12.11.2001 . « , , . , , . , . 2% , - , ( 7,4). , 6 0,1 ( 7,4), 50% . 7,4), 20% , 0,1 8–10 0,9% «Leica» (2% (18–20° ) –22° , ) 30 (pH 7,4) . 0,1 20 , (Dakopatts; X907). 10% 30 . , (Milab, 1270, 1:400), (Peninsula, N31030/L3, (Affinity, IHC 8601, 1:200) 1:200). . , , -GARIgG, 30254, Jackson) 1:100 2 . (pH 7,4) (3:1). «Axiophot» (Zeiss, ) 3- . . (GAR IgG, Dakopatts Z421) , 1:100. 12 ( . , (Dakopatts Z113, ) 1:100), 12 . (Amerham). 400× . . , , . , 7 6- 11- ( ) . «Bioscan-NT» . 1%, 5%. ( ), ( ) , 5 10 , ( ). 10 400× [ . ., 1990]. , . 2 . 3 (25–35 ) . ., : (36 . ., 1973]. ( ) [ 25 ), . ., 1953; «Statistika» (Version 10.0). – . – ( ), (m), ( ). 0,05. , , . . (U) 8 , , 2,1±0,19% 3,6±1,07% . ( – ± 12,3±1,58% , ( , , , . – , , . ., 2001]. , [Nasu T., 2000; Jimenez B., 2002]. 1. – , ). , 1). 9 , . [ Arciszewski M., 2006; Helen P., 1984; Heym C., 1993]. , . , , ., 2002; . , , , 2). 2. – , (25–35 , (36–60 ) ) . , , , , . , , , , . 10 (9,6±0,79%) (1,8±0,32%) (15–24 ), , . - ( 10–20 ) . [Jew J., 1985; Heym C., 1994]. , , . , , . , , , , Y, [Chiba T., 1989]. , , . . , , , , . (p<0,001) , 3). , . , , 2003; Schultz J., 1996; Zatta A., 2008; Zhang J., 2006]. , , , . [Kuzume K., , 11 3. – , , . . in , vivo [Kuzume K., 2003; Zatta A., 2008]. [Schultz J., 1996]. [Heuser M., 2000], . , . [Raevskaya O., 1988], [Przew ocki R., 2001]. , , . 12 [Eide K., 1992; Zhang J., 2006]. . , [Chiao H., 1996], , [Ustinova E.,1995]. , , . , ( (p<0,001) . , 4). 15–24 , 4–5 , . , , , , [Mascorro J., 1994]. 4. – , , 13 , , , , . , , , . , [Erspamer V., 1981] [Chiao H., 1995]. [Eklund B., 1977]. , [Laurent S., 1985; Xiao R., 1997]. [Maryanovich [Guarini Fisher L., 1985]. A., S., 1994], 1985; , . , , [Hua F., 2004]. , , [Polak J., 1986; Guarini S., 1989]. , , , , , . 1. , , , , [2, 3, 5, 18, 20]. . 14 2. , . , (9,6±0,79%) (60,7±4,28%) 62,1±4,81%, (28,3±2,65%) . (39,3±4,91%) – 87,1±3,53% . 11,1±1,87%, 1,8±0,32% , . , [2, 3, 5, 18, 20]. 3. , , . , 12,3±1,83% . 36,1±4,92% 24,7±1,44% , (2,1±0,19% 3,6±1,07% 26,4±2,11% ) [1, 4, 6, 8, 12, 14, 16, 17, 20]. , 4. . (22,7±2,41%) (25,4±3,97%), , . (12,3±1,20%) , . , , , , , , , [1, 4, 6, 8, 12, 14, 15, 21]. 15 5. , , . , , , . , [1, 4, 6, 7, 11, 13, 18, 19]. 6. – , , – , [1–4, 6, 7, 9, 10, 13]. 1. « » ( , 110-0812) [20], , . « » . ( 17.12.2012 .). 2. , , , , . 3. , , . 16 4. . 17 1. , . . / . . , . . // . . – 2013. – 1 (43). – . 110–113. 2. , . . / . . , . . // . – 2014. – 1. – . 110–112. 3. Saet, O. S. Expression of substance P in human stellate ganglion / O. S. Saet, V. V. Roudenok // Medicine. – 2015. – 1 (88). – P. 12–14. 4. , . . / . . , . . // . – 2015. – 3 (90). – . 29–33. 5. . , . . . / // . ; . 460–462. 6. , : . . . . . , . : 2 ./ . . – , 2008. – . . . . 2. – . . / : : . . 62- . 63- . // . ; 2 . / . . . , 2009. – . 2. – . 347–348. .– . . . . . , 7. / . . , . . , . . , . . // : . . . . ; , . . . . . 110. / . , . . . . .- . , . . , 2011. – . 186–189. .– 8. / . , . . , . . , . . : .- . . . . . . . 229–231. . ; , . . . / . , . 110. . . . .– . // . , , 2011. – 18 9. Alteration of vasoactive intestinal polypeptide, substance P and neuropeptide Y expression in the human thymus after chronic thyroiditis / V. Roudenok, A. Nikitski, A. Sokal, A. Hancharov, A. Nerovnya, O. Saet, T. Hrachukha, W. Kuehnel // Global Strategic Center for Radiation Health Risk Control : proceeding 6-th Int. Sympos. Nagasaki University Global COE Program. – Nagasaki, 2011. – P. 103–104. 10. , , / . . , . . , . . , . . // - , : . .- . . . . , ., . 100. 2014 . – , 25–26 , 2014. – . 196–200. 11. / . . . . , . . , . . , . ., // , : . ., . 100, 25–26 . 2014 . – 12. , . . / . . . , 2014. – . 193–196. . , . . . // «9- : » : . ; ./ .: . . , .– . . . , . . , 2014. – . 318–322. 13. , , / . .C , . . , . . . ., . 10, ) . . . , . . 14. , . . ] / O. C. . . // : . .– , . . / . . . , 2010. – . 320–327. , . . // ; : 90 19 : . 2013. – , . . 3. – 1 . . . / ; .: . . .– : (DVD-ROM). – . 154–156. . . . . . , 15. Sayet, V. Expression of met-enkephalin in human stellate ganglia after acute myocardial infarction / V. Sayet // Folia medica cassovensia. – Vol. 64, 1. – 2009. – P. 56–57. – [Proceeding 1st International Student Medical Congress Kosice]. 16. Sayet, V. Expression of met-enkephalin in human stellate ganglia after acute myocardial infarction / V. Sayet // 17-th Int. scientific conf. students and young doctors : abstract book. – Gdansk, 2009. – P. 122. 17. Sayet, V. Expression of met-enkephalin in human stellate ganglia after acute myocardial infarction / V. Sayet, V. Roudenok // Proceeding 5-th Int. scientific conf. students and young doctors. – Bialystok, 2010. – P. 137. 18. , . . / . . // . . . . . .– , 2011. – C. 25. 19. Expression of met-enkephalin, vasoactive intestinal polypeptide, galanin and neuronal nitric oxide synthase in human stellate ganglia after acute myocardial infarction / O. Saet, A. Nikitski, A. Sokal, V. Roudenok // Proceeding 6-th Int. scientific conf. students and young doctors. – Bialystok, 2011. 20. Plasticity of neuropeptides in human stellate ganglia after acute myocardial infarction / O. Saet, A. Nikitski, A. Sokal, V. Roudenok // Eur. J. Med. Res. – Vol. 16. – 2011. – P. 107. – [Proceeding 22-nd Eur. students’ conf.]. 21. Saet, O. Up-regulation of substance P in human stellate ganglia after acute myocardial infarction / O. Saet, A. Sokal, V. Roudenok // Proceeding 8-th Bialystok Int. Med. Congr. Young Scientists. – Bialystok, 2013. – P. 235. 22. 110-0812 : . . ; 09.08.2012 / , . . , . . – 2012. – 6 . . . . , . . ; , . . : . . . – 20 : , , , . 45 : . 59 : ( ( ) i ( ( ) ). : . , , , : «Olympus», , (Zeiss), ), «Axiophot» «Jung» (Leika). : , i . , , i , . i i . , , i , . , i i - . : i , ; ; . : , , , i , , , , , i , . 21 : , , , . : 59 45 . : ( ( ) ( ( ) ). : , ), , , . : «Axiophot» (Zeiss), «Olympus», , «Jung» (Leika). : , . , . , . , , . , . : ; , ; ; . : , , , , , , , , . , 22 SUMMARY Saet Olga Samuilovna Expression of neuropeptides in human stellate ganglia in normal conditions and in case of acute myocardial ischemia Key words: stellate ganglion, neuropeptides, expression, acute myocardial infarction. Object of research: autopsy material of human stellate ganglia in the age of 45-59 years old. Purpose of research: to identify expression patterns of substance P (SP), met-enkephalin (met-ENK) and bombesin (Bom) in normal human stellate ganglia and after acute myocardial infarction (AMI). Methods of research: indirect immunohistochemical, histological, morphometrical, statistical. Equipment used: universal photomicroscope “Axiophot” (Zeiss), light microscope “Olympus”, automatic images system and computer processing data, automatic cryostat microtome “Jung” (Leika). The results and their novelty: for the first time a research of SP, met-ENK and Bom immunoreactivity distribution in normal human stellate ganglia and after acute myocardial infarction was conducted. It has been revealed that in normal human stellate ganglia heterogenic population of neurons and nerve fibers immunoreactive to SP, Met-ENK and Bom exists. Appearance of population of small neurons immunoreactive to SP, met-ENK and Bom is a pattern of AMI. Increase of SP, met-ENK and Bom expression following AMI reflects the role of neuropeptides in functional regulation of cardio-vascular system. Recommendations on application: the new data obtained can be used as normative during medical and biological scientific research; in patho-morphological and medico-legal diagnostics of cardio-vascular diseases accompanied by acute myocardial ischemia; in the development of new drugs; in medical education. Area of application: cellular biology, cytology, histology, neurochemistry, normal and pathological anatomy, normal and pathological physiology, pharmacology, legal medicine, cardiology, internal diseases. 23 15.02.16. . . . 1,39. .- 60 84/16. . «Times». . . 1,28. 60 . « ». 113. : . 1/187 , 6, 220006, ». , 18.02.2014. . ,