2 279 212(13) C2

ÐÎÑÑÈÉÑÊÀß ÔÅÄÅÐÀÖÈß
RU
(19)
(11)
2 279 212
(13)
C2
(51) ÌÏÊ
A01H 4/00
(2006.01)
ÔÅÄÅÐÀËÜÍÀß ÑËÓÆÁÀ
ÏÎ ÈÍÒÅËËÅÊÒÓÀËÜÍÎÉ ÑÎÁÑÒÂÅÍÍÎÑÒÈ,
ÏÀÒÅÍÒÀÌ È ÒÎÂÀÐÍÛÌ ÇÍÀÊÀÌ
(12)
ÎÏÈÑÀÍÈÅ ÈÇÎÁÐÅÒÅÍÈß Ê ÏÀÒÅÍÒÓ
(21), (22) Çà âêà: 2004121131/13, 13.07.2004
(72) Àâòîð(û):
Äåìåíêî Âàñèëèé Èâàíîâè÷ (RU)
(24) Äàòà íà÷àëà îòñ÷åòà ñðîêà äåéñòâè ïàòåíòà:
13.07.2004
(43) Äàòà ïóáëèêàöèè çà âêè: 10.01.2006
Àäðåñ äë ïåðåïèñêè:
127550, Ìîñêâà, óë. Òèìèð çåâñêà , 49, ÔÃÎÓ
ÂÏÎ ÐÃÀÓ-ÌÑÕÀ èì. Ê.À. Òèìèð çåâà, ïàòåíòíûé
îòäåë
âåðõíèé - èç òåðìîóñàäî÷íîé ïëåíêè, êîòîðóþ
ïîñëå óêîðåíåíè ðàñòåíèé óäàë þò. Çà ñ÷åò
äàëüíåéøåé àêêëèìàòèçàöèè íàäçåìíîé ñèñòåìû
ðàñòåíèé äî èõ ïåðåñàäêè â íåñòåðèëüíûå óñëîâè óâåëè÷èâàåòñ âûõîä ðàçìíîæåííûõ in vitro
ðàñòåíèé. 1 òàáë.
R U
2 2 7 9 2 1 2
(57) Ðåôåðàò:
Èçîáðåòåíèå îòíîñèòñ ê âûðàùèâàíèþ
ðàñòåíèé. Ïîñëå âûñàäêè ïîáåãîâ â ñîñóäû ñ
ïèòàòåëüíîé ñðåäîé ñîñóäû çàêðûâàþò äâóì ñëî ìè ïëåíêè. Áëèæíèé ê ðàñòåíè ì ñëîé
âûïîëíåí èç ïëåíêè, ïðîïóñêàþùåé âëàãó, à
Ñòðàíèöà: 1
RU
C 2
C 2
(54) ÑÏÎÑÎÁ ÐÀÇÌÍÎÆÅÍÈß ÐÀÑÒÅÍÈÉ in vitro
2 2 7 9 2 1 2
(56) Ñïèñîê äîêóìåíòîâ, öèòèðîâàííûõ â îò÷åòå î
ïîèñêå: SU 1400557 A1, 19.12.1985. SU 1340652
A1, 30.09.1987. RU 2201071 C1, 27.03.2003.
R U
(45) Îïóáëèêîâàíî: 10.07.2006 Áþë. ¹ 19
(73) Ïàòåíòîîáëàäàòåëü(è):
ÔÅÄÅÐÀËÜÍÎÅ ÃÎÑÓÄÀÐÑÒÂÅÍÍÎÅ
ÎÁÐÀÇÎÂÀÒÅËÜÍÎÅ Ó×ÐÅÆÄÅÍÈÅ ÂÛÑØÅÃÎ
ÏÐÎÔÅÑÑÈÎÍÀËÜÍÎÃÎ ÎÁÐÀÇÎÂÀÍÈß
"ÐÎÑÑÈÉÑÊÈÉ ÃÎÑÓÄÀÐÑÒÂÅÍÍÛÉ
ÀÃÐÀÐÍÛÉ ÓÍÈÂÅÐÑÈÒÅÒ - ÌÑÕÀ èìåíè
Ê.À.ÒÈÌÈÐßÇÅÂÀ" (RU)
RUSSIAN FEDERATION
RU
(19)
(11)
2 279 212
(13)
C2
(51) Int. Cl.
A01H 4/00
(2006.01)
FEDERAL SERVICE
FOR INTELLECTUAL PROPERTY,
PATENTS AND TRADEMARKS
(12)
ABSTRACT OF INVENTION
(21), (22) Application: 2004121131/13, 13.07.2004
(72) Inventor(s):
Demenko Vasilij Ivanovich (RU)
(24) Effective date for property rights: 13.07.2004
(43) Application published: 10.01.2006
(54) METHOD FOR in-vitro MULTIPLICATION OF PLANTS
shrinkable
film.
After
plant
rooting,
heat
shrinkable film is removed.
EFFECT: increased yield of in-vitro multiplied
plants
due
to
further
acclimatization
of
aboveground part of plants.
1 tbl, 1 ex
R U
2 2 7 9 2 1 2
C 2
C 2
(57) Abstract:
FIELD: plant growing.
SUBSTANCE: method involves planting sprouts in
reservoirs
comprising
nutritive
medium
and
closing sprouts with two film layers. Layer
closest to plants is made from moisture permeable
film, and upper layer is made from heat
Ñòðàíèöà: 2
EN
2 2 7 9 2 1 2
Mail address:
127550, Moskva, ul. Timirjazevskaja, 49, FGOU
VPO RGAU-MSKhA im. K.A. Timirjazeva,
patentnyj otdel
R U
(45) Date of publication: 10.07.2006 Bull. 19
(73) Proprietor(s):
FEDERAL'NOE GOSUDARSTVENNOE
OBRAZOVATEL'NOE UChREZhDENIE
VYSShEGO PROFESSIONAL'NOGO
OBRAZOVANIJa "ROSSIJSKIJ
GOSUDARSTVENNYJ AGRARNYJ
UNIVERSITET - MSKhA imeni K.A.TIMIRJaZEVA"
(RU)
RU 2 279 212 C2
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Ïðåäëàãàåìîå èçîáðåòåíèå îòíîñèòñ ê îáëàñòè ñåëüñêîãî õîç éñòâà è ìîæåò áûòü
èñïîëüçîâàíî â ïëîäîâûõ ïèòîìíèêàõ.
Èçâåñòåí ñïîñîá ðàçìíîæåíè ðàñòåíèé in vitro (Äåìåíêî Â.È. Ìèêðîêëîíàëüíîå
ðàçìíîæåíèå ïëîäîâûõ è ãîäíûõ êóëüòóð. Ì.: Èçäàòåëüñòâî ÌÑÕÀ, 1997).
Îñíîâíûì íåäîñòàòêîì óêàçàííîãî ñïîñîáà âë åòñ íèçêèé ïðîöåíò ïðèæèâàåìîñòè
ðàñòåíèé â íåñòåðèëüíûõ óñëîâè õ.
Íàèáîëåå áëèçêèì òåõíè÷åñêèì ðåøåíèåì ê çà âë åìîìó èçîáðåòåíèþ âë åòñ ñïîñîá,
ïðåäóñìàòðèâàþùèé ïîêðûòèå ñîñóäîâ ïëåíêîé Parafilm. Îäíàêî ïðè èñïîëüçîâàíèè ïëåíêè
Parafilm ðàçâèâàþòñ ñâåðõîâîäíåííûå ðàñòåíè , êîòîðûå ïðè ïåðåñàäêå â íåñòåðèëüíûå
óñëîâè ïîãèáàþò, âñëåäñòâèå ÷åãî ïðîöåíò ïðèæèâøèõñ ðàñòåíèé ñíèæàåòñ (Thomas M.
Contolling vitrification of petunia in vitro. Programm and Abstracts 1988, p.758).
Çàäà÷à ïðåäëàãàåìîãî èçîáðåòåíè - óâåëè÷èòü âûõîä ðàñòåíèé, ðàçìíîæåííûõ in vitro,
çà ñ÷åò àêêëèìàòèçàöèè íàäçåìíîé ñèñòåìû åùå äî ïåðåñàäêè â íåñòåðèëüíûå óñëîâè .
Äë ðåøåíè óêàçàííîé çàäà÷è ñîñóäû ñ ïîáåãàìè äë óêîðåíåíè çàêðûâàþò äâóì ñëî ìè ïëåíêè. Ïåðâûé ñëîé èç ïëåíêè, ïðîïóñêàþùåé âëàãó, íàïðèìåð öåëëîôàí, âòîðîé
ñëîé - ïëåíêè, íå ïîãëîùàþùåé âëàãó, íàïðèìåð òåðìîóñàäî÷íà ïëåíêà. Ïîñëå ÷åãî
ðàñòåíè îñòàâë þò íà óêîðåíåíèå. Ïîñëå óêîðåíåíè ïîáåãîâ âåðõíþþ ïëåíêó óäàë þò,
ñðåäà ïîñòåïåííî âûñûõàåò, íàäçåìíà ñèñòåìà àêêëèìàòèçèðóåòñ , ãðèáíà èíôåêöè íà
ïîâåðõíîñòè íå ðàçâèâàåòñ , ðàñòåíè õîðîøî ïðèæèâàþòñ â íåñòåðèëüíûõ óñëîâè õ.
Ïðèìåð âûïîëíåíè ñïîñîáà.
Äë ðàçìíîæåíè in vitro èñïîëüçîâàëè çåìë íèêó ñîðòà Ðåäãîíòëèò. Âåðõóøêè ïîáåãîâ
çåìë íèêè âûñàæèâàëè íà ïèòàòåëüíóþ ñðåäó Ìóðàñèãà è Ñêóãà, ñîäåðæàùóþ 0,5 ìã/ë
ÁÀÏ+0,01 ìã/ë ÈÌÊ+30 ã/ë ñàõàðîçû, âèòàìèíû (ïèðèäîêñèí, òèàìèí, íèêîòèíîâà êèñëîòà
ïî 0,5 ìã/ë), ìåçîèíîçèò - 100 ìã/ë, àãàð 6 ã/ë. Ïîñëå ï òîãî ïàññàæà êîíãëîìåðàò
ïîáåãîâ äåëèëè íà îòäåëüíûå ïîáåãè, âûñàæèâàëè íà ïèòàòåëüíóþ ñðåäó, ñîäåðæàùóþ 1/2
ìàêðîñîëåé M. Ñ., 1 ìèêðîñîëåé M. Ñ., ñàõàðîçó 20 ã/ë, 0,2 ìã/ë ÈÌÊ. Îñòàëüíûå
êîìïîíåíòû, êàê íà ýòàïå ïðîëèôåðàöèè ïîáåãîâ. Óêîðåíåíèå ïðîõîäèëî ïðè 16-÷àñîâîì
äíå, èíòåíñèâíîñòè ñâåòà 3-4 òûñ. ëþêñ, òåìïåðàòóðå 24-25°Ñ, ïðîäîëæèòåëüíîñòü
óêîðåíåíè - 30 äíåé. ×àñòü ñîñóäîâ äë ñðàâíåíè ñ ïðåäëîæåííûì ñïîñîáîì çàêðûâàëè
ïëåíêîé Parafilm. Äðóãóþ ÷àñòü çàêðûâàëè äâóì ñëî ìè ïëåíêè, ïðè÷åì ïåðâûé ñëîé
ñîñòî ë èç ïëåíêè, ïðîïóñêàþùåé âëàãó (öåëëîôàíà), à âòîðîé ñëîé - èç òåðìîóñàäî÷íîé
ïëåíêè, íå ïðîïóñêàþùåé âëàãó.
Ïîñëå óêîðåíåíè ðàñòåíè , çàêðûòûå ïëåíêîé Parafilm, ïåðåñàæèâàëè:
1. íåïîñðåäñòâåííî â íåñòåðèëüíûå óñëîâè , ïðîöåíò ïðèæèâøèõñ ðàñòåíèé ñîñòàâèë
50%;
2. ïîñëå óäàëåíè ïëåíêè Parafilm ñîñóäû îñòàâë ëè îòêðûòûìè â òå÷åíèå 10 äíåé.
Ïîñëå ïåðåñàäêè â íåñòåðèëüíûå óñëîâè ðàñòåíè ïîðàæàëà ãðèáíà èíôåêöè .
Ó óêîðåíèâøèõñ ðàñòåíèé â ñîñóäàõ, çàêðûòûõ äâóì ñëî ìè ïëåíêè, ñíèìàëè âåðõíèé
ñëîé èç òåðìîóñàäî÷íîé ïëåíêè è îñòàâë ëè ðàñòåíè äë àêêëèìàòèçàöèè ïîä îäíèì
ñëîåì ïðîïóñêàþùåé âëàãó ïëåíêè. Ïîñëå çàâåðøåíè àêêëèìàòèçàöèè ðàñòåíè ïåðåñàæèâàëè â íåñòåðèëüíûå óñëîâè . Ïðîöåíò ïðèæèâøèõñ ðàñòåíèé ñîñòàâèë 89-90%.
Òàáëèöà
Âëè íèå òèïà ïîêðûòè íà ïðèæèâàåìîñòü çåìë íèêè â íåñòåðèëüíûõ óñëîâè õ
45
Òèï ïîêðûòè Óêîðåí åìîñòü, % Ïðèæèâàåìîñòü â íåñòåðèëüíûõ óñëîâè õ, %
Èçâåñòíûé ñïîñîá: ïëåíêà Parafilm
95
50
Ïîñëå óêîðåíåíè ïëåíêà ñíèìàåòñ , ñîñóäû îòêðûòû 10 äíåé 95
Ãðèáíà èíôåêöè Ïðåäëàãàåìûé ñïîñîá: öåëëîôàí-òåðìîóñàäî÷íà ïëåíêà
89
95
Òàêèì îáðàçîì, ïðåäëàãàåìîå èçîáðåòåíèå ïîçâîëèò çíà÷èòåëüíî óâåëè÷èòü
ïðèæèâàåìîñòü ðàñòåíèé â íåñòåðèëüíûõ óñëîâè õ.
50
Ôîðìóëà èçîáðåòåíè Ñïîñîá ðàçìíîæåíè ðàñòåíèé in vitro, âêëþ÷àþùèé óêîðåíåíèå ïîáåãîâ â ñòåðèëüíûõ
óñëîâè õ â çàêðûòûõ ñîñóäàõ ñ ïèòàòåëüíîé ñðåäîé ñ ïîñëåäóþùèì âûðàùèâàíèåì
Ñòðàíèöà: 3
DE CL
RU 2 279 212 C2
5
ðàñòåíèé â íåñòåðèëüíûõ óñëîâè õ, îòëè÷àþùèéñ òåì, ÷òî ïîñëå âûñàäêè ïîáåãîâ â
ïèòàòåëüíóþ ñðåäó ñîñóäû çàêðûâàþò äâóì ñëî ìè ïëåíêè, ïðè÷åì ïåðâûé, áëèæíèé ê
ðàñòåíè ì ñëîé âûïîëíåí èç ïëåíêè, ïðîïóñêàþùåé âëàãó, à âòîðîé âåðõíèé - èç
òåðìîóñàäî÷íîé ïëåíêè, êîòîðóþ ïîñëå óêîðåíåíè ðàñòåíèé óäàë þò, à ïåðåñàäêó
îñóùåñòâë þò ïîñëå èõ àêêëèìàòèçàöèè ïîä ïëåíêîé, ïðîïóñêàþùåé âëàãó.
10
15
20
25
30
35
40
45
50
Ñòðàíèöà: 4