ЭКСПРЕССИЯ ГЕНОВ RHOA, SEMA3B И CKAP2 ПРИ

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ЭКСПРЕССИЯ ГЕНОВ RHOA, SEMA3B И CKAP2 ПРИ РАЗЛИЧНЫХ ТИПАХ ЛЕЙКОЗОВ
. . .,
Климов Евгений Александрович,
, . . .,
. .
,
EXPRESSION OF RHOA, SEMA3B, AND CKAP2 GENES IN LEUKEMIA OF DIFFERENT TYPES
Eugene Klimov, Doc. of Sci., Group Leader, Faculty of Biology of Lomonosov Moscow State University, University Diagnostic
Laboratory
SEMA3B
.
.
.
.
RHOA, SEMA3B CKAP2
RHOA CKAP2,
.
CKAP2
-
ABSTRACT
Transcriptional activity of RHOA, SEMA3B, and CKAP2 genes was assessed in blood samples of leukemia patients and
healthy donors. In blood of healthy donors, RHOA and CKAP2 gene expression was not detected, and a low SEMA3B gene
expression was observed. Significant elevation of expression of all three genes was shown in case of acute myelogenous leukemia.
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"Educatio" II (9), 2015
ИЦИ СКИ
79
АУКИ
In cases of remission of acute lymphoblastic leukemia and myelodysplastic syndrome, expression of all three genes was not
detected. Long isoform of CKAP2 gene is highly expressed in most analyzed types of leukemia.
:
,
,
, RHOA, SEMA3B, CKAP2
Keywords: leukemia, gene expression, RT-PCR, RHOA, SEMA3B, CKAP2
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«TrТгШХ RNA PrОЩ»
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-
CKAPқ
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CKAP2-F: TTCTGAATGCCTGAACTTGAT, CKAP2f-R:
TTAACATCATGGGTTGGATCT, CKAP2s-R: AATTCCGAATTGTCTACTACACTG;
95° /Қ5”, 5қ° /Қ0”, 7қ° /30” (F) қ0” (S- ).
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"Educatio" II (9), 2015
.
RHOA, SEMA3B
1. Etienne-Manneville S., Hall A. Rho GTPases in cell
biology. Nature. 2002;420(6916):629-635.
2. Lessey E.C., Guilluy C., Burridge K. From mechanical
force
to
RhoA
activation.
Biochemistry.
2012;51(38):7420-7432.
3. Parri M., Chiarugi P. Rac and Rho GTPases in cancer
cell motility control. Cell Commun Signal. 2010;8:23.
4. Vial E., Sahai E., Marshall C.J. ERK-MAPK signaling
coordinately regulates activity of Rac1 and RhoA for
tumor cell motility. Cancer Cell. 2003;4(1):67-79.
5. Kamai T., Kawakami S., Koga F. et al. RhoA is
associated with invasion and lymph node metastasis in
upper urinary tract cancer. BJU Int. 2003;91(3):234238.
6. Liu N., Bi F., Pan Y. et al. Reversal of the malignant
phenotype of gastric cancer cells by inhibition of RhoA
expression and activity. Clin Cancer Res.
2004;10(18Pt1):6239-6247.
7. Aznar S., Fernandez-Valeron P., Espina C. et al. Rho
GTPases: potential candidates for anticancer therapy.
Cancer Lett. 2004;206(2):181-191.
8. Pan Y., Bi F., Liu N. et al. Expression of seven main
Rho family members in gastric carcinoma. Biochem
Biophys Res Commun. 2004;315(3):686-691.
9.
. .,
. .,
. .
.
RHOA
/
.
. қ006;40(5):865-877.
10. Yazdani U., Terman J.R. The semaphorins. Genome
Biol. 2006;7:211.
ИЦИ СКИ
80
CKAPқ (
АУКИ
)
.
11. Roche J., Drabkin H.A. The role of semaphorins in lung
cancer. Clin Lung Cancer. 2001;3(2):145-150.
12. Castro-Rivera E., Ran S., Thorpe P. et al. Semaphorin
3B (SEMA3B) induces apoptosis in lung and breast
cancer, whereas VEGF165 antagonizes this effect. Proc
Natl Acad Sci U S A. 2004;101( 31):11432-11437.
13.
. .,
. .,
. .
.
SEMA3B
.
.
2009;43(3):439-445.
14. Maouche-Chretien L., Deleu N., Badoual C. et al.
Identification of a novel cDNA, encoding a cytoskeletal
associated protein, differentially expressed in diffuse
large B cell lymphomas. Oncogene. 1998;17(10):12451251.
15.
. .,
. .,
. .
.
CKAPқ (LBҚ),
.
. қ00қ;36(6):985-989.
16. Jin Y., Murakumo Y., Ueno K. et al. Identification of a
mouse cytoskeleton-associated protein, CKAP2, with
microtubule-stabilizing properties. Cancer Sci.
2004;95(10):815-821.
17. Bae C.D., Sung Y.S., Jeon S.M. et al. Up-regulation of
cytoskeletal-associated protein 2 in primary human
gastric adenocarcinomas. J Cancer Res Clin Oncol.
2003;129(11): 621-630.
18. Tsuchihara K., Lapin V., Bakal C. et al. Ckap2
regulates aneuploidy, cell cycling, and cell death in a
p53-dependent
manner.
Cancer
Res.
2005;65(15):6685-6691.
ФАРМАКОЛОГИЧЕСКАЯ КОРРЕКЦИЯ ФЕТОПЛАЦЕНТАРНОЙ НЕДОСТАТОЧНОСТИ
С СИНДРОМОМ ЗАДЕРЖКИ РОСТА ПЛОДА
Клычева Ольга Игоревна
,
, .
. . .,
,
. . .,
.
Лазарева Галина Анатольевна
,
, .
Хурасева Анна Борисовна
,
, .
PHARMACOLOGICAL CORRECTION OF FETOPLACENTAL INSUFFICIENCY WITH FETAL GROWTH RETARDATION
SYNDROME
Klichiova Olga, postgraduate student Department of Obstetrics and Gynecology, Faculty of Postgraduate Education, Kursk
State Medical University, Kursk