61 31/00 577.3:615.272.4 ! ' ( " !## $%! & () ) ) ( ) 14.00.25 – * + , -, 03.00.04 – . ! / 0 -# #$ $ / . ) # &! -* + , - + # ! &!# " /! !# , &! # &#1! $ / $ /! +!$ 0 # *! $ / *! – 2001 ! +!$ 0 # / : # " . ., # " . . .........................................................................................................................7 1. 2 ( ......................................................................................13 / 1.1. %# /% $!" / ! 1.2.1. #/ $!" / !$ / !# - . #/ $ #/1 -+ , $!" / $ #/# ! $!" / $! %#1! #/ / .!## / ! ! /!+ 1.3.4. #$ /! 0 - #/!# ) 1 1 $ , / $ #/#1+ ..........................................15 * 0 -. ! # 3+1 $ #/ ............................................25 !# - + &! # , $ /!+ ! ! # , $ - 0!# !$ #!# " !# - ! ! $ 1/ ......................................29 # , !# - in vitro ...27 !# $ $ ...31 + $! %#1 ..................................................................................................38 !# - . # / .1 # 0 0 1.3.3. -. # , . / /1 $ - + $! %#1 1.3.2. /! #/ $ #/ ! #/ /!+1 ! ! $ #/# " 1.3.1. 2. / - ..................................................................................................17 1.2.2. ) 1.3. ! !+ ........................................................................................................13 !+!##1! 1.2. / !##1 $ +!/ $1 ! / # / 0! + $! %#1 !$ . $# $ %# , /!+ ...........................................43 # - ..................................................................52 1...........................................................................................52 # !..........................................................................................................52 !# ! !# ! !$! !# ! + .........................................................................................53 + ................................................................................................53 #0!#/ 0 (2,4-$ # / *!# . # %#1 !$ #!# " %3 # !+ N- ), $ 3 # ........................................................................53 2 !$! !# ! #0!#/ 0 !4! / , ! , 54 . . / 2-/ " / "...........................................................................................................54 !$! !# ! !$ 0!# $! 6 # - ##1! + &! 8**! / / / / &! #/ ! # / #/ $ / / . . / . . / !$! !# - . # " 2,4- / "( - 2 ), $ - 0!# /1 %#1 /!+!.................57 54 #- ) /!+! .................................................................................57 +!# + /% +!/ $ !$! !# - # !+ 2,4-$ # / *!# + $! %# " / + 3 0 - + " + Cu2+-3 ! %#1 , $ 3 # ( $! 6 # - !$ #!# " ) $ - 0!# 3 &#1 $ / ) ..........................................................................64 +!#!# - + $! %# " + " #$ 0 !" + /% # /! %# / . # /!+!.........................................................61 !$! !# ! !##1 ) + ..........................................57 !$! !# - !4! / , ! , " # /! %# ! $ %$!, $ 54 /!+1 $ - 0!# + %# " + $! %# " +!# + /% +!/ $ #- ) 3.2.1. # !+ / , $ 3 # %3 3.1.3. # ! +!# + / +!/ $ %3 + $! %# " 3.1.2. ) , - ...........................................55 +!#!# - + $! %# " - !4! / # ) $ # / -*!# 3.2. $ #/# , $!" / # /! %# - 0!# 3.1.1. ) / # !$ #!# -...............................................................54 / + 3 0 - $ #/# , $!" / 3.1. ##1 $ !# ! +!/ $1..........................................................................................56 3 . / 3. #75, !# - . # $ !# - /!+1 ) # # ! !# -.........................................67 %#1 !$ #!# " .!## /!" # 0 0 + / 0! .........................................................................................................67 3 -# ! 3.2.2. . # 3.2.3. #0!#/ 0 %#1 * * / $ !$ #!# " + /! 54!, 0! ) 3+ 6# /% %3 # - . *! #1 # 4. 0!# # ! $! $ /! %# / /!+1 ) + $! %# " + " #$ 0 $ #/# , $!" / # !+ + $! %# " !# ! ................................................................74 Cu2+-3 + .!## /!" #/ %3 # # # # ) - ! /!+! .....................77 / !##1 ! # !* * / $ / # 1 + .........................................................................................................................83 4.1. -# ! $ / * %#1 ) # !# ! #! % . # $ / -# - + -# ! , $ * !# ! #/ $ / ) %#1 !$ #!# ", %#1 $ #/ + !# ! $ #/ # ....................83 $! 6 4 . # # # # # %#1 $ / !# ! . # %#1 /!+!.................................93 !# ! . # %#1 $ / ...................................................................................93 -# - / + .!## / # #/ -# - / + ) %#1 *!# %#1 * . # /!+!.................................83 + %# " + $! %# " .!## / 4.2.3. !# ! ...................................................................................90 ) ) 4.2.2. # # -3!", # # %#1 .!## / 4.2.1. * %#1 $ "#1 ) 4.2. -# - .!## / 4.1.2. $ #/ + %# " + $! %# " .!## / 4.1.1. #/ # # # !# ! . # %#1 $ / ...................................................................................95 -# - . # + ! %#1 . 3 54 $ / ) . # 1 + /# ....................96 5&!# !......................................................................................................................101 1 $1 ............................................................................................................................106 4 %3 ) ## " /! / 1 .............................................................................108 6!# ! 1. !$# # ! & # ) ! ) $ 6!# ! 2. + /% +, 6!# ! 3. / . $ ! ,# /1 - 8** 0 !#/1 * * / $ . # & " 1. &#1 !$ #!# ", . 3 54 # 0 0 $ - /! %# / . # $ ! ,# /1 6!# ! 5. %#1 #$ 0 #$ 0 . # ) $! / # / %#1 ## + # +!6$ !# $ ........................136 # ! MES – NaOH # !#3 ## + ( !# 5, / !$!...............................134 #0!#/ 0 !" -# ! . *! #1 $ ! ,# /1 ! -0 !$ #!# " #/ ## + $! 6 # ! ## " $ / + !$ #!# " ## + # . 0 %#1 %#1 #$ 0 -# ! . *! # , $! 6 # ! . # #!"# " # 1 6!# ! 4. /# %#1 $! 6 # - 0!/ / # / #0!#/ 0 !" ) + / . # / !# - ........................................................................................126 #0!#/ 0 ) " 3# & + / #0!#/ 0 " + 3 !#3 ) / / / &! +, !# 5. ......................137 6,0) # !$ #!# " # ! !#3 . # 1 +, !# 5. .............................................138 5 - – #/ $ #/(1) – #/ $ #/# - – / #1! * +1 – !4! / , ! , – 1 # -6 $ – , / / – , $ – 2,4-$ # / *!# # ! ! – + $! %# - – # / # 1" $ ! ! – ! – . $# %$!, $ # ! " #1! !# ! /1 $ $$ + / 3 $ . . / 2-/ – *!# 3 #+!/ %# ! !# ! - / %* / 5+ #! 0!#0 !#$ + # /!/ !$#!! *+!/ &! M – SD – / #$ /# ! / SEM – / #$ /# - 9 . – /# / /!+ – 8/ + / , * - - !$ # 0 – !+ / " , $ 3 # #!# 14!##1! 6 – " $ # " /!/ 3 / &! – – /# - /! #1 # 3 " + . . / $ 3 $1 – – $ 54 ! 2-/ 8**! / – . . /!+ # - / ! #!# ! !$#!, 8** 0 !#/ 8 / # 0 !4! / 6 $ #! #1 : #+ !"#$ % # &!' ( 4! / / . # !6 , /#1 1 , # 3+ # 8 .#1 + / / 6 1 6 54!" ) !/ . !$! #! !/ 5&!# 4! / !## + - # , ! 1 + , / !, !$ / !.! / -## 5 , 3 $ - $ 0! 1 6 3#!$!-/! %# / / # ! ! $ #/# !0 * &! ) 3 ), *! +!#/ /% - $ . , #!# - 131 !/ * # 0 ! ! / - /$! %#1 , / . 1- !#1 / . / / # - . - #/ ## . / 54 $#1 #/ $ #/ . # - , # 3+ + 6!/ . $# # / $ . ;/ , + ! , # 3+ . ) 5 &!3 , / # /%5 !$ / %# / ! $ - , &! & / &$#1 $ #/ . !+!# # !# .9 0! #/ 3# , / !##1 + - #- # + 6!/ .1/% &! / ! ! !+ 0! 50 !6$!# " . #!&# + / ,!, , .! % +!#!# !+ #/ /! # - /! %# ! & /! %#1 !+!## , . %# 3 $ 0 / / -## , # 3+!. - #1 # %# " + / !##1" $ . # # / -4!+ # /%5 #!+ * # 0 / #!" , &! #/!/ &! #/!# $# $ $!" / %#1 / # + %# , * # 0 $ + 3 $#1! + - !$! ! %+ $! 8 1# 3 + ! !# ! !/ / -# - , # 3+ , ) ). / -4 - 3 . %9 , & # +!6$ !# - ( !$%, 1 - # -. ##1! !6$!# " $ /- &! , # 3+ 3 9!# 5 . $# /% . , / " / $# " &!- !$ / 4! / $ ( !# 5 # / /!+ ( / &#1! /! %#1 6 , # 3+ #1! * +1 0 !6$!# " &#1 3 , # 3+ - , # &!# - #/!# /! %#1 # ! , #!#/ + +!/ . 4! / $ ! , /% - 4!# - + !.! $# $ — / !/ / !## , 16 + 5&!# !+ #! / , # 3+ ) #! 3+ 6# &! / ! . 3 5/ - ! # !+ ! (3 $ , - -54!, - #! /7!+ !+1+ 0! 3 # $ #/#1 ! + &! / #1! #1" + /! +# , !$ 6!# $ - +!$ 0 # , +! # 3- +!#!# - 3# & - / . 1- #!#1 #! / 1! +! # 3+1 $!" /!$ #!# ", + / # #!#/1, # 7 - -54 & ! / 1 #/ $ #/#1! "- + ! ! !4! / - - -!/ - #! . $ +1+ " / . $# - $ $ 4 3$ !/ &! 4! / !##1+ * / %3 # ! " # - 6# / #!# / 1 + 6# #! / % !$ #!# " $! !+!## +! # 3+ 0! ) +!#!# ! + 6!/ 3 +!# / !$ +!#%9 !/ -, 3 /% - # . 5$ !/ - # * #! #!$ / / &# . -54 + ! 0 -# / - / !$! / #/!3 .!## /-+ 1! ! 1- !##1+ + &! # / !##1! - + &! 3 & /% $# - &#1 - - /! # !+ ) - , /!&!# !+ 3 / !/. !- 3 ##1" !$!# - 19!## - #/!# - -/% $ - # /% ) $ #/#1! $ $!" / $ #/#1 / /! # / 0! - + &! ) / " ! #/ !$!- #0!#/ 0 # / 6! & ! !+- 3+ 6# /% 3+!#!# !+ !$ #!# " " / ,# + , 3+ 6# - / !/ - / 1/1+. 3 $ #/#1 # 3 &!#1 !# - !#/# / . " / . !+ " / , - -5/ . / / ! ! $# " 0! /1 ,$ !, $#-9# " $!#% * / - / +!/ -3 +!6$ 1- !# - # #/ !+!## , 8/ #/ + 4%5 /! %#1+ # 1 / + -0 $ #/# , $!" / ) # + # ! 3 + / $ ,$ .#1 $ 6 /! %# / - !# + / -!/ - . / #1! # #1+ ## " !$ / ), 3 # - in vitro , # 3+ , / 4!# - #0!#/ 0 - , 4! / -/% # ", # # /% ) !3#1+ $ - + $! 3+ 6# /% # ! + , /!+ #/!# 6# / 6! /+!/ /%, &/ + /!+ " / !4! / # - /! /- /! %# + / ! ! - () !$ - 8/ ! #! $ #/#1 /% /$ !##1! 8**! /1 #0!#/ 0 - /! %# + + , - . /% 1- $ $ + 6!/ $ # #, 1 .!## / $!" / 6#!"9!" +!/ $ 8/ + !# - & 5- !$ #!# " ##1+ . 3 + & ! #/ /!+ / &! / + &! 6# " # , + 3 . / ## , # * 0 $ #/#1 , " / " / + . 1- -/% # + !3 %/ / . -54!, /!+ ! ! - !# - #/ /! % ! $!/ %# / !. !+1+ + $! %#1 /! # - 3 in vitro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« + # / ) . 0!# $! 6 # 5 10 + $! %#1 . # /!+ #/!# # / ! !- %#1 !$ #!# "», $ / ! !# ! < 10–2001 / 6 *! " $!+ !"). 3 . / #1 +!/ $ &! .!## /!" #/ /!+! # - 2001 ,., 1$ ## ! $ #/# , $!" / # ! ! - ! +!#$ 0 + &! $! %# - /!+ $ * * / $ !# - /! %# # 1 « 1- !# ! !$ #!# " 0!# + $! %# " - 54 # !+ $! 6 # 5 , + 6!/ 6 /% /! /- " / 0! %3 #- ) $ #/#1 : /! 54!, + 0!# " / " +!$ 0 #- +»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– 17 ( #*! !#0 +!6$ # , + #*! !#0 -4!## " 100- !/ 5 », +!$ 0 # , # /-. - 1998 ,.); # $#1+ & / !+ « . $#1! $ ! » ( + !# , 19 – 22 !#/-. - 1999 ,.); # # &# " ! , $ " +!$ 0 # *! !#0 , 16 – 17 -# «2 " $!+ , , $, 5 – 9 ( -4!## " 125- !/ 5 $#- - 2001 ,.); # 5-" ) 4 # , - — # «) . !+1 +!$ 0 #1 . , 6$!# - " / 1/ " » !+! ", 11 ! /!/ +. " " # 0 # %- 1 . !3# &! - / $# , - /-. - 1999 ,.); # # &# " . . / + #*! !#0 ! » () 4 # , 16 – 20 21-, #$ +!#- « , ( + ! - 2001 ,.); # $ $1 #- , , $, &!#1 #*! !#0 / !## " +!$ 0 # " - $!+ ( !+! $!#/ + $1 , , $ ! - 2001 ,.); # 66-" ! , 19–20 , &!#1 2 9 /$! !# - 2 " + + &! , , $ # / / # ( * , 26 ! / 0 - . 6$!# # , &! . , $ #*! !#0 / - ! - 2001 ,.); # 3 !$ # - .4! / 1999 – 2001 ,,. +! /# + 3 !$ # ", " *!$ * + / !## " +!$ 0 # " , $!+ . - / 3 !- . '"* !1** ) /!+! $ & ! / 0 . # 6 # &#1 &! * '6 4 +*%% & !1** ! / 0 - 3 6!# # 139 / # 0 $!# -, .3 !$ . /. /! / 1, , # -, $ &!##1 , !3 %/ / !3 %/ / , 3 %3 ## " 156 # / ##1 1, / + 9 # # , /! / -4!## " . / !##1 # 5 + /! !$ 5&!# -, 1 $ , /! / 1 . 5& !/ 195 ! / 0 - 5 / 12 / +!/ $ # ", , 1 . 6$!# - /! / 1 / &# , 3# # 7/ . 6!# ". 39 /!&! / !##1 0 + 21 # +. - "!,! 1. . !"#$ % # 2 *% ! " 1.1. !&% , $$(7 2& 2!&! , % !$ * %*+!$ $(4 + 8% ,* 4 0! ! +!/ . / &#1! / ! 0 $ ! / $ $ vivo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in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in vivo - -5/ - . # 8 .#1 [14, 49, 62, 103, 170], 3 / /1 [31, 47, 90, 98], # , !/ #1! * +1 . $# .! 3+ , . !3#% / 6! # , !+-, / #! - 3 ##1 3 . ! # ", # /! %#1+ * / + # 9!# - 6 54!" % $ #/# - #/ !$1 + &! + +# , ! 3 # + #!#/1 !$ #!# -+ / * /, &/ # 1 , # 3+ 9!# - . , /!" $ !/ 3 ## , . # 3 # #!8**! / / #) , , / / + 1 $ #/#1 3 $ ##1 0! -+ !/ - # / / -$ +!/ $ / 1" 3 $# , &! 3 . / ## , / + &! 6 /% - * + /! ! #/ $ #/#1 , # /%5 !## , .!## /-+ / !/ / 54 - !# 3 , &! 3 +# + , .1 - !# - ## #, + !$ + &!" / ,3 - # "# / - 4! / !## " . !+ " - -!/ - /- /!+ + $! # -) 4! / -/% in vitro, - # #, # 1 " / + . +! / , , $ , #/!# !$! !##1+ # . ! 9!# " / + #1 )( - -!/ - ##1+ . 3 + 3+ 6# /% 138, 158, 181]. + /-, - % [1, 33, 101, 151]. , !$ !/ / 6! /+!/ /%, &/ , 3 # + / / $# /!". !$! !##1+ / !. !+1+ .1 5 ( %# /% 0! !# !$!# ! .1 # * 0 - . # +!6$ # !/, $# $ #/ !$! !##1+ 6#1+ 8/ / / / 5/ #! . - ! 0 !$# #/ -54 " / . /% . /1. ! $!/ %# - $ " # - / ,!#!/ &! / # %# , # / , &! !$ !$! -5/ " / . !$ #!# ", * + / 1 $ . # !/#1+ + 9!#-+ $!" / /% 3+ 6# /% 3# & /! %# , +!#-!+1 4! / !## 5 !$ / 6! +# , # /% .1&# , !/ 3 ##1! / " 3 . ! # - , # / * # 0 !# !+ #/!# , &! [41, 154]. - 6$!# -, 0! 1 # / , 4!# - /!&!# - 5. , 3 . ! # $ + /% ,!## , /% #/!# / - -!/ - 3 , -3#!# ! [23, 29, 64, 104, 176, 182, 193]. # / !& 5/ - # #/ .#1 / + 6 9 $ #/# , . $ #/# , $!" / &#1 , " / !##1 3 + $!" / #! , &# + " / !$# ! , $1 !# $ 6! - +!6$ #/ +# , - [26, 67, 106, !$! !##1! .!## - . ", &/ + 6!/ 1- $ #/# , $!" / - !# . / -, / $ #/# , $!" / - [9, 78, 79, 97, 158, 160, 175]. !$ $ + /! %# , , .!## 1- -/% # - + ! ! $ $ $&! # /%, &/ # 8/ ! & ! # !/# , !4! / $ - # #, #/ 14 # #, # 1 , /. $ #/#1 , #! . - 6! /! %# #! / " / ,# 3- +!/% + 6# /% # /% !$ /! %# " 0!# - !# - #/ + , # & / + 3 /% $ #/# , $!" / " # &#1 . 6# / / / /! %# : «2 ! /! 5& !/ - 5/ . 3 #1+, $ !# * # 0 - . 3 # - #! %3- , 6. / !$!] -/% - ! !# - ( .#1" !" / /! %# , !$! -!/, " / , . !$ / - ! /- + # 0 !, 8/ + #& !, !$! !# ! $ # 2. 4 !/ . / /) / / /. / - - 1- !# - #/ ! # 0 / # 0 0 + 9!#% . 4! / !## " /! !# # !/# ! !4! / - -/% #/ -$ 8**! / 15 -, .# , %3 5/ - $ - 1- !# - !# - - -!/ - 8 - !# ! 8/ , /! 6# /% / , , 6 / + 9!#%5 8 1, 3# & /, #0!#/ 0 !" . ! -/ /- 0! ! # + " 1 !/ %3 !+1" / 1! #! / % ) . / #0 -, # -/% - $ / %3 - , #! %3- # 3 /% $ #/ — 5. , $&! $ + 3+ 6# , ( - -* # 0 - !$ #!# -, 3 !# - # 6 !/ - ! 1 , . $!/ ,! !$! !##1! . "/ & #0!#/ 0 , &! +! / , , +# , ! $ + &! - / 1 , . $6 [94], 6$!# - & / , , $# 4 5/ - & !$! !# ! # . !! " / , / #! +!#%9!" /! !# , — ,— $6!+ [94]: « #/ $ #/#1 0 " + /# ! , # # -. » [8]. ,# . / / , $ / ! # / ». ;/ 3$ !/ - %3 !/ - # / » [17]. >. . .# / . - # 3 " !$! !# !, &/ 5&!# #! / % /% . +!#%9!# . !! $! -[ 3 ## $!" / 6. $ #/1 — 8/ $ $ ! 0 $ #/# , + . $#1 % 8 , &! / !$! !# !: « #/ # 6!# / . /! %# " # 5 # 3 # ! « #/ 5/ 2. !$ #!# -, — #/ !/ . !! 3 ! & .#1 #-/ -. !$! -!/ #/ !4! / , ». 9 $ / - !, ! 8/ , 3+ 6- -. /- /! + # « $&! !$ - ! !4! / . , /+!/ /%, &/ 0 - . .= , # & /! / !, # , - %*+!$ !7 0! -/% ! 0 !$ !/, $# +!$ 0 # 1 3 3 &!# $ $ #/ ». $ #/# , $!" / !$ !+1+ !4! / + ,& 4 $$( 2& +% !," $*- ' !$ * 1.2. . + 9!# 3+ # / #, . ,/ $ #/#1! # - !# 0 0 ) $ - / +1+ 3 . - !$ #!# -+ / . ) Fe2+-3 + " # 0 0 # /% - -5/ / # *! - -!/ $ #/#1! -!/ - # . !! 8**! / [192]. ) . ) &! Cu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in vivo 1 4 5/ # !6$!# - .! / in vitro. +1! / !+- - $ $ #/ + + 6!/ +!/% + $ #/# " / !$ / &!#1 8/ !.! /#! !# ! !4! / #/ - $ [192]. [186]. /! %# , /! %# , + .! , !/#1+ $1+ + / /, / - - %3 !+ " + 9!# - - . 3 4 4 !/ / + , / + , / .1/% !#% . % . 3+1 &!+ &!#% - $ !# / % !4! / + &! - 3 / / , # . H 212/43 & / &# ! + / # . 0 [74], ) - $1 [100], / - . # - $ #/# 5 3+! / (OCl–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in vitro, /! !# 3 *! $ , " / # , + 6!/ / 6! !4! / " / .1 5 &! !$% $ / / &# / / / , / / %*+!$ ( 2&-4 9 + 8% ,*-. "!%%*:* !1*-. ! # ,/ -. # /% /!+ . , # 3+! .7! / ) + 6!/ — 1 [6], # / -4 " + +!#/ /! /- $# !$ !+1 !# 1 -3 # #/!# . $ #/#1 / , +! , . / 54 " # 6!# 5 #/!# &#1 $ #/# !, # !- ; /! /- 19!# 5 -0 !$ !/ / 6! 3 .1 /%, &/ /# 9!# / -, + &! , . / 54 " ,$ + 6# .#1! # 6 /% -+ , $!" / #! . $ + , $ - .! !&!# - !0 !## , $!" / 0! 1 -, ! .# /%5 # 6 /% #- /!+ , /.!., # .7! / , # #! /% - / # + $, /! /- !4! / / !/ / !## 8/ + $! !# 5 / +! , # -, . $ 54 ! + &! !## , ( – 1 ! in vivo, + , / 3$! !#1 # : – / $ #/# ! $!" / +!#%9!" , # 3+ . - /! % &#1+ - /1 !$! !## " / &! /! -+. ) -+ , $!" / -. /!"9 - 0! !" - * 0 - # 3$! /% # $ !, # - # # 1, !/ # , . ( $#1 / * + 3 -!/ , + 9!#-+ $!" / +!6 / &#1! $ #/# " - ( $ /1 % -!/ & - #/ + &! !9!# 5 , " !$ #!# - " / !##1 " / . $ # 1 . 3 /% / % 0 ", , /! . * 1– 0 - #! #/ / 1 !$ 31 /% !$ , - 3+ 6# /% - !4! / !$!- / !/ / !/ 1, / # * 0 - $1, , $ /% # 1! ! !4! / -!/ , $# + /1 ! / #1! - !+!##1+ &!/ + # - . /% 3 + # & 5 / -3% +!6$ & !+ &!/1 ! -3-+ , !$#-- # 0 0 5 / 18 ! !1 #/ + &! !- $ #/#1 " / / !$! !##1 * # 0 - " # %- /! / #1 $ ##1 [8, # #1! / –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– + ! - 3 / 1 /!, - 3 / ! – .# / 1 #, . - !&!#1 - / #1 +!/ 6! . ,# . !$ !/ /, #! / 1! 3 # 3 +!!/ -, % $ .# , $!" / #/ %3 . / /% - # ! + &! " H2O2, , # &! , $ Fe2+-3 + ! ! *! + # ) # ! ! $#1 +!/ -!/ [107] !$ $# + ! !3 & 5/ / #! / % . !#$ + # /!/ #1+ #/ # - 5/ $ , " / [56]. #/ / !#$ ™) [172] # !• H #1, . 3 $ #/# , $!" / $ #/# , $!" / 3 # / # 19 %#1 + • ! 1 #!- H # / / $ / - ! +!- ! - / - Fe3+ - - ! - #! / 1 ! # - $ #/# ! $!" / + -!/ H2O2 [92]. $ #/# ! $!" / # ! / /- /1 H2O2 [92], # # / 4! / !## , &/ #1, " /% . 3 - 5+ #! 0!#0 $ , " / # ! #/ / / #/ -, 0! . # + ! . 3 !$ 4 + +! # 3+ + #/ ( - -/% #! / % / - !+ [92]. . ! # - !$ #!# -, . - / 6! - -!/ - 8**! / $ $ 6! 3 +! # 3+ / +, &/ + 8/ - -5/ 8**! / # ! 3 -+ - # + [82, 92, 155]. 1,10-. / *!# #/ # 3 # 8-, $ # ! !/ Cu2+-3 +1 !$ !/ . / /% $ - , / !& 54 ! 3 $1 # 0 / ) # / , 0!, $ #/# ! $!" / .; + " / + -0 / 5/# 5 -. .#1 /!#/#1" ! 4! / !## . # #, # 1 ## , ) #-!/ / !/ / 54 + /! !". / $ #/# + $!" / Fe3+ / . - -5/ - !$ , !+ ! $! !# ! &# + &!+ Cu2+-3 Fe2+ / . $# " / + , " / .) ) $ !! #/ $ #/ + -3 . 3 - Fe2+- #$ 0 / (; / " / . ##1! ! /! / #1! 8 !+!#/1 + ! $ " !4! / , # , # ++ . 3 54 + $ #/#1! ; . 3 /% ! 0 ! + — + / ! !/!#$ !/ # $ #/#1 !$!# -, + # !# , # - + !$! !##1+ (3 $ ##1+) +! # 3+ + $!" / ++!#/ , 0 - #! # #1! / !4! / + - $ 54 ! * 9% ! .#1 / + $ .# - & /1 !/ - !# ! / 3 /%, &/ #! * # / !## , ! !$ #!# " [99]. $ / . #/ /!, - $ #/1 -+ , $!" / - " / ) $ #/# ! $!" / . # 1 !4! / - / # $ . $#1! $ – $ 6#1+ / + + 1 ! 0 $ 1 A•, 3 54 ,+ , /8 + X• A• $ 6!# - 0! /!, - . /% / . / # -) $ %- %#1" $ / /# 9!# " #0!#- " /! # - !- 3 + $!" / 5/ / # + 6!/ #! # /%5 #, . $ !$ #!# " [6, 37]. #/ –$ # / + / + # /1 5/ $ , X• – $ +!6 / &#1" / 0 " ! , 0 $ . )! ! : AH + X• → A• + XH, ,$! 6#1+ / + + #1" # 0 / . $ $ - . &#1! ! 0 $ %# ! [6, 95]. / # /% /% 8**! . !! .9 # - , / ! - -/% ) !$ / /! 0 1 /!, - " / ) $ #/# ! $!" / # #1+ +! # 3+ + #/ 1. !4! / *!# %# " , 4! / 8/ " , + - " 1 - -!/ - 3 + $!" / $! ) !+- ! $ / 5/ &!#% / #1 +!/ + ! - $ $ ) [107], 1 / . 3 - .!## . 3 54 - *!# %#1! . [6, 51]. ! / 1! # ! / 3 + $!"/% +!- *! - # ) - $! 6 4 ! ,!/! 0 &! / . $ + + , # +1 ! - *!# 54, 111, 138]. ! !4!- # $1 [5, 83, 113, 157, 159], / /! %- #1! *!# 1 [5, 40, 135, 145], 8 / ,!#1 [43, 122, /! !". - *!# %#1 ? 3 $1 [154]. ! / # # [128], 3 $#1! 1,4- . - 3 $#1! # */ [6, 40, [140], * –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–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–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e(n+1)+, / " $ Men+. / # * + & ! $!" / - +- .#1 - -/% $ #/#1 *! " / [6, 37, 54, 111, # [179], # +!/ α-/ 3 + # [40] #1 ! ! $# " *! #!- !/ - 2,3- !/ , + &!- #!#/ /! /- !#/# / [40, 53, 54, $ #/# , $ #/# ! $!" / !#/# / [10, 53]. ;/ - !# ! # /%5 . # " /1. !# - (Me(n+1)+) / 1" .!# . 3 # 5 / $!" / - !$#!" ! 0 & /% $!, $ 5 / 25 .! 1! !- – !" , - [92, 188, 189]. Men+ $!, $ . # . # $ .#1" 0 $ , ! -3 # 3 6!# ! !/ - . # /- !/ - /% $ / # ! # +!/ ! 0 3 . $#1 / / # ! /% , # !+ $! # : [6, 37]. / 1" 3 /!+ # % $# + .!## / -/% Men+ $ Me(n+1)+. ! !/ &! #!&# , &#1 * / [6, 37] 3 &!#1 . 3 . 3 54 - - .# !/% / + , 1 9!" /! !# / " $ Men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α- *! .!# # /! %#1 - + ! #!#/ 3 0 ! 0 #/ $ / / &# " ! 0 - -/% ## " $ / / &# " !" / . &#1 ! α-/ / 4 .) . !! 1 !/ -, ! α-/ $!" / # - . $#1 #0!#/ 0 - #/ *! .!## $ .# !.! . $ 5/ 0! #1! ! 0 %#1 $ $ $ $!" / !+ / - ## , !# - 3- #/ $ #/# ! $!"- & ! -$ *! /!+ 19!, &!+ $! ! 0 " # /% α-/ / + 6!/ *! . 6$ 5/ - 10–6 – 10– #0!#/ 0 (Wi) $ #/# - !$! [6, 37]. *! # 6 - - -/% - $ #/# ! 1 6!## ! # # & %# + 8/ ! 3+ 6#1! +! # 3+1 & / - α-/ ! 0 - + #$ 0 19! 5⋅10–4 – 4⋅10–3 , , *!# # /% α-/ %, &/ 8**! / #0!#/ 0 - 1, . 3 54 !- 5& /% - !, - $ . %9 # / ! # !$ %#1! *! #/ ## , # . !! 8**! / /% . 3 / / / #! / + 6!# ! ! 0 " 3 # , &/ 8/ + /+!& . %9! ) .1 *! - / #0!#/ 0 /! %# , # + $! - . / / " / !# .# /%5, &/ .1 . $!/ # . 5$ /% - / + -0 -, *! !$!. $ #/# , $!" / ## " [6, 181]. ) 8/ + $ #/#1! $ / !# - [6, 37]. !, .3 ! .2. 2 !# " / 0! #1 + (1998) [6]. +! / , , α-/ / # # 0 / , /% *! Cu (II) $ Cu (I) / / /! #1 3+1 .# /% *! 3+! $ #/# ! $!" / $ #/# ! $!" / , 3 !/ !, 3# & /, + 6!/ , /% -3 ## ! . !! *! ! α-/ - 1 1 -3 # &! *! #0!#/ 0 / , !4! / !&!# !+ / 4! / !## 5 26 3 !/ - *! / # +. !, # 1–2 - $ , # 3+!. /% / !/- . / # -$ 6 - + %# ! -!/ 14 – 42 + -3 ## ! *! % .#1 α-/ ! #1+ / [111] .# 5$!" +!## 8**! / - ! #/ 9 : .#1 ™ $ #/# , $!" / 3$ &/ 8/ # /%5, ! +1" # #/ #0!#/ 0 - α-/ $! 6 # ! α-/ / !## , " [92]. 3$ . !! # 3 , &/ $ #1 Cu2+ $ Cu+ [54, 111], - -54 / + !, /! %# " # 6!, &!+ - ! 0 , - @/ ! 8/ , - -!/ - [26, 27], $# / # 19! 6! ! . 3 % / 6! ! 3 ) &#1 /! 5/ , .!## /- #/ ", / !/ !$!# - . / 54 3 ##1 8/ ! ! " / + $! %#1 31 5/, !$! !# - + "#$( %*% 4( 2 & !$ * %*+!$ $ 8 ! /!+ # 3+ , [6, 35, 37, 54, 3$! +!+. # . 8/ + ! 0 " /! %# (1 $# " / #1, 3+ 6# / $# . / . !!) /!- 6# /% 1- !# # / , - $ , " / $ #/# , $!" / - - 3 & !+1 # #, # . !! $ .#1+ $ - 1- #!# - !$ #!# " !$ / *%$ 9 -!/ - %3 # ! - + &! !$ / , " / + [89, 94] " *%" $*- "*2*+ , +"- 1 $ * *,$ % * 7*4*/ % *7 % +*$ $*8 , 2( !7 in vitro # 6! / !## /% +! # 3+ $ #/#1+ !$ /!+. 1.3. , # 3+ !# - (#!- $ #/# , $!" / # . %9!" 8**! / &# , 3 & !+1 $ #/# ! 3 &!##1+. - !# 5 #1, #! . $ + /% !$ #!# ". . . - $ %# ". / 3+ 6# / /!+ / &- 5/ - # , # 0! 3# . 3#1 $ #/# , 3 3 # # 0 /! 54 +!+. ##1 + $! %#1 !$!##1! . !+1 ! %+ #1+ . 3 + - ! / , + ,!##1 0! 1 ) . / 5/ # * #! ! + #!#/# !# - + / 1 .1 . / / , # 3+! $# " * 3 ) - 9 +- – /%, &/ % . %9!" & /%5 # # & %#1 8/ !+- 54!, " / + . 3 +, + 6# , +!#/1, $ #/# ! #! 3 &!#. $ #/#1 !$! !##1 !8 $ , $# " #0!#/ 0 - , &!+ #/ - !# - 3$. 1.2.1). $ , &/ $ #/# ! $!"- " +! # 3+ 8/ , - !# - 4! + # / – 4 & 181]. 3 # , &/ $1, &/ $! !/ 3 &!# ! 8/ " +!/ +, ! , .1 3+ 6# /% ! " *! . !! 1 + &! /! !" ( +. + &! $!" / " 3 # $ #/# ! $!" / # " $ - α-/ / # , #/ !, / / / #! !, $ #/# , $!" / ! / , " 3 + 3 !4! / 3$ !/ 3 ! /#1! .!## /!" $!" / + &! 6# / - 3 &!# 6 27 + - !4! / # + / +!6$ . , &! #! #/ - /! /- : $ - 3 &!# - +! , # 3+! / !. !/ - !$!- # !8 ! +!#/ 3#1 (# %3 #- $ , " / $ #/#1 /1! $ / / - / 1 ) &/ / / . $#1 /! + # / /). ) 0 / + $ + / ! # 6. %3 + / !$ #!# " # # -». 3$!" / /, $# - /!+1 ( . $&! $ #/# 5 ) !$!- #!#/ : +! # 3+ ,!#! - !# - ( /% #1 /# - + $! %# " $!/! / #/ + 9!#% ! # 1 # $6 [94] /! %# , ), # .!## - %3 5& /! %# + $! %#1! 8 - ! 5. - +!/ $ /!+ $ $! + 6# , .! #/ /!+1 [8, 21, 89, 92]. #0 $ / &# / ! /! /- "#!" +! ! $ + &! , . / #0 # 1 !/ - # #1, 2. #1+ - -!/ - #! / % stress + &! # / !.- # , &! +!#/ . + , / .1/% 31 5/, &/ « !+ .3 ! /! / . !$! !## " 0! 1 ) 5& !/ $ , " / [21]. + &! ! & - -# #1! (. /! %# " !$! !## , 8/ " + /! ! # #,), / 6! $ - 3 &!# - +! # 3+ /. [21] #/ 0 &#1" !. # /!+1, )8 -+ , $!" / /! %# !# - 6 #1, $ - 1- !# - # -# - . . + , # 3 0 " / ( ! /!" # !+ 0! , / ) 5/, # /% 6- - (source of oxidative .#1" -/% - .- /! %# " + $ * - , /! %# " $!, $ 0 - /.$. ++ $ #/# " / /% $ $1 # / , $ 6# $! 6 /%: (1) 0! . / / !# -; (2) #!#/ + # " 8 ! 0 8/ + ) +!## /. # -/ #, . , +!54 + $ .#!! + #/!# $!" / # # " 54!! $!" / ! ! / #!#/1 / $ - !# ) .!## / 0! 1 ) in vitro, , ! #1 $ - # &! , & 4! , # !/# —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») + , / +!/% - #!$ / / /# - /! + $ # + &! 0! & /% - %#1! "# + / + + + / /! -+ «$ !# 1 (8 %$!, $ / $ — . # 8/ + ! # $1), !#1, 0! , $ - 3# & /! %# +!#%9!" , $$ /1 !$ #!# ! 3+ 6# , $ , 3# &!# ! /! # - + —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in vitro, .!## /%5 +!+. # , +!6 / &#1! ! /! # /% , # 3+! - -5/ - %#1! $ , +!#/ -3 ##1 # / ) / / + ! 0 ") ! / . / / ) #/!# #!# -, $ - 8 . !+, 1. *%" $*- "*2*+ , !+1 4! / !## ! # 1. 14!##1 6 .4 #1 !+ + ! - # , +! # 3+ / () = ) (# # ! 30 ! ! # , !# - !$!# " 3 [92, 95, 147]). #!# - ) +!6 / &#1! # -+ $ – ) = $ $!" / + 6!/, 3 # $ /1 ) %#1!, / !+ / + / ) ! !$ #!- $ ! ! ! $# " ! [37, 95, 147, 180]. . $! ) !# -. #/!# ! $ !#/# / - $ / - $ .#1 $ # $!, $ 5/ 5 &! !$% 8/ $-4 , . 3 # - /! # $ - , $ 3 ) = , $! 6 4 !- 4 . !! 4! / !## - & /% - -3!" [95, 147, 180]. # # - & /% ! $ - $ $ ! , 5/ - , + * 0 # . !! 50 #$ #1! $1). +!/ -/% - # % $ $1 - $ $ "#1 $ , ! 1+ . $ %#!"9!, $ /! %# #! / "& %#1! (, $ 6! !3 ", ! 0 +! / , , , $ $ / # - $ ! /% - !+ #! $ / /% - $ # % - -5/ - /# $ /1 ) 5/ 1 / &! $ ## / 6! + %#1 , & /% 3 " ! 0 ) = , $ , $ - +# , & !$ #!# ") / . / 1 (# .# /%5 +! , / . # !##1! ( $!#/ - %#1! / %#1! &# /%5 $ - 6 &#1! #!&- !$ #!# -) . $ 1 , # 3+ [95, .!## #! . $ + $- +! # 3+ 0! # " ! 0 : 147, 180]. - . .!## /- &! # /%, &/ ! # $ # 0! ) # 0 0 5 $ ) + /% - ! ! # , !# - + ! 0! ! . $#1 0! + 6!/ 0! ,/ # + / ,! 2 /! # - !# - 8/ " + ! $ 1) = 3 / & 1 + 6!/ . 3 /% - !/ #!&- : (1) 6$1" 3 / 1 + 6!/ # 0 + / !##1! .!## / !# ! # 1 + ! /! # - ! 0 " ) . ! / 1.3.2. '% &! ( +"- 4 + "#$(7 %*% 4 2 & 1. , " /% 3 8/ + &#1 / &! %3 $ # - . / / 6$1" ) ) !$ !/ & /1 /% 1- !$! !##1 0! - . *%$ 9 $ #/ . $ !/ 31 ) = . *%" $*- "*2*+ , %3 # - $ / / &# !- /! #1+ ! / +$ / - # / #!$ / / # #, (/ . . 2). . / /) –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– &# , [94]. + 6!/ / &!## , / $ # - !, . ) $ .# ! #! 4!" * 31, / , 6! ! !3 %/ / !$! !## , % / !$! -!/ -, $ $ - ! " / + : / / &!##1 / , ) !$ 54 + / ! & -+ $. !/ # - $# , & - #!#/ ) .! !& – %3 $ 6!# . $ /% &!#% 3# & /! %#1! / – - " / - / # / 1 +!+. #. 0! - . . / /1 ! ! # , !# - $ , +!#!# ! / 1 #/ $ #/# " / . / /) ;* - / !, /% #/!# , + 1 / $ , # &!- /! - #1 1 $ $# - , # &! * 3 $ . / , / 0! $ 8+ % $# " * 3! ) 6 $ . / 1 , / 0 / ) # " !#3 -$ + $! %#1 /! /- /!+ $ - 3 &!# - !4! / +!#!# - # 5. ) 3 /! / -5/ 0 " + &! " / ) = . . ! $ / !$ / 1 /% -# ! # . / / ! /! / !##1+ [37] !# . / / + , # 3+!. # , + ,!#- %3 , &! !+1 / $, / # /% ) ) / # / .!## / 1 #!# 14!##1 6 #1 3+ 6# % # & %#1" ! . $ $-/ $ - + / 0 , # 3+ , $ * !# -. . / / / / , # 3+ . ) , / +!#%#1+ $ / ) , # 0 [37] - . 3+ 6# /% ! 0! $ 3 &!- %3 "- # -, $ * . !$ " %#1 # - , # 3+ ( [37] $- $# "). + # 5/ - !# -. A , #! / 1 /$! %#1 / / !/ , # 0 .#1 # ! ! 0 ") 3$! + * 3. /+!#/ !. 5/ $ . 3# & /! %# " /! !# + $ * 0 ,/ 8**! / !$ !+ , !/ . /% / #!" [6, 37] !# - 8+ %+ /!- 0 2 . / /) 0! 1 * 3! .!## / $ / . / #1 / #$ / 3 $# " 3 &!# - . 4!##1! + 0! 1 $ #! - #1 + / - + /+!#/ 3 0 /+!#/ 3 0 5 . / / -/% # /! #1 /! # ! 4! / 5/ .9! ) . B 4! !##1" / $#1! + # ( – 2 $6!//) . ", + # /! %#1" !4! / . %3 # - [20, 22, 111, / 54 4! / !## ! #! !,$ /! /- . 156, 163] / -# / $$ 54 ! - 8**! / - ! $!/! ,!#/ + 6!/ + ! , #! [181] -# - # $# " # / % !$ !+ , /! # 6 3# + # ! !" +!6$ # /% + 0! ! ) /% , / #!#/ + # !$!# . !# -. -. -# !+ " / + # .!## / ! $ !+ / / 3+1 . !! $ .#1 $ - 0!# +!#!# -+ * 3 &! $ ) , # 3+!. $ - 1- #!# / !, 3 8**! / $ $-/ $ - # ) &! /+ #!#- . / / &# $ /# 5 ) ". ) / / 5/ ! /! / !##1! 3+1 !# %3 5/ - &!/ . !4! / $ / ) 9 / !. +1! +!/ $1 1$! !# - !, .) / 0! /! / &!# -. !$ !+ , $#1" . . / /) !## + !#,+5 ##1! +!/ $1 $# " * 31. / / / !/ /! - +!#!# - 6# /% -, , # .1 .1/% #/!# %3 # /% ) / 1! /! 3 5/ -, 3 + $!" / ! + /! #1 ! # - #!#/ &!# - + # +!#!- 3+!6- # 5 $ ! . !# - ) $#1 + ! / 1$ - !/ $ ##1 . / /) ) ! /1 .!## / $#1 +!+- $! 6 / . # $#1" . , # 3+ / .) !## + –+ +1 / + $ . ! 6# /% 3$! !# - +!# +1 #!*! +!#/ / 3 + " # 0 0 0 - !$ !+ , $ #/# " , !4! / + 6!/ 2 .) / #$ " ) 3 # - 2 3+ / &! #/ / . $!/ # . 5$ /% - ! +!+. #1 $ #/# ! $!" / # 0 0 / -# / $# , # /# , , #! !,$ /% + !4! / . , / .1/% *! +!#/ / # " #$ 0 $ 0 - 0! 1 ) %3 5/ - +!+. #1 NADPH [105, 167] NADPH- + -+ $ * - #1 $ - 1- !# - #/ - +1 +!/ . ) / !$ !+ - /% + / $ 0 / /! !# * * - #$ 0 , . 3 54 + - [3, 25] . !, + + - 450. +!#-5/ $ - " / !## &! /- ) .) + 450-3 4! / !## (± 30%) 3+!#-/% - 3 -5/ 3 & /% - #$ 0 %3 + 450-3 + [34, 38] &!# -. # - 3+ 6# 0 / 6 $$ 54 ! - #!#/ . *! +!#/ / .1&# ! #! / #!" ) . /! $1 /! %# " 0! – , # # / 0 / # -0 / – +!+. #1 + / # !/#1 / $ !+ . !/ &#1 /! / /! #1+ $ - / / 5/ ! /! / !##1! &!/ . /!* / " +!#!# - !$ !4! / #! . $!/ # . 5$ /% - # - ! /! / !##1 !/ * %#1 . B 4! [125, 155] . " / !## !, +!/ . # 0 0 / +, / /!+ " +!/ + 3+!#!# ! #/!# . /. # / . / /) / !##1! +!+. #1 .!## / ) / -# / / # # -. – !. +!+. #1 "#1! - +! # &! +!## + $!" / # /% / +1 -0! ). /# / 5 " 0!$ /! %# - #! +, . 3# 3 , + 5. % 6# /% &!# - +# , !- / !- [28] /! %# - #! [30] 6- /% &!- + # / $ 3 &). - / . / %- , / /% /! %# - / $ !+ +!+. #. ) !# -. 1 / / / "& -. /# . /# 3+ 6# /% !# -. / &# /%, %# /% ( / #! +. /! %# , / &! /! / +!#/ / . /# %# , 8 ! / , / , ! &# /%, 8 $ .!# . 0!$ +1). #1+ 3# ##1 - - / . $#1" . / ## /% !$! # - +!+. # .! # -. ! 1 –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i, ! 3+ 6# /% . ! # ! #6! 0 - ! .#1+ !/ ) !# ! 0! ! 3 & , -3#!# ! . !! 9 %3 !/ -. !/! ,!#/#1" $ ##1+ 3 [28]) !$ / / / . / ##1" / +( 0 3 !4! / # ) 3+! . + 8 / 3 5&!# -. . - & ! / / &#1 &! / /! -. # -. ; / 3 - 2 !! 1 - / . # /%, &!+ 3 &!##1 +. ! / + ) &!# 1" 3. ! %- . ! (10–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• – 2 . $ !/ 1 / / 3 ## ! – # - 6# - 4! / !##1+ "#1 8 , / . ! %# /% +!#/ . $ $!" / ), , $ !+ #,(• H), - $ " 8 ! / * .# /%5 [77, 92], $# 2 - # 0 / - , + #1+ . 3 + # 0 0 / ( NOO–) [60, 92, 162, 180]. /- 2 %*+-!$* $-&!+* !" • - .!## 8/ /!+1 # 0 0 #- $ # / 1 . . 3 54 +!#!# -. 5/ - , ## " 3 , # 3+!. # - + 9/ .#1 $- # 2) # , ! / $, / # 0 2, % , / / %3 *%" & + 0! / , + # &! , # 3+! ! 0 ! 6 #1 $ - $ . / !/# , # -!/ - & /1 /%, # / !/ / !/ / # #,! + . , 6 1 , # 3+ , - 1 2 %# " / # - $ + + , !$ #, !/ 5&! 5 !# ! [166]. . $ !/ 1 !/! 38 ! !/ " ! + &! 4!# ! " / , $ # /%5 [75, ! $- $ • ( • !## – 2 2 # 3 /# 9!# & !3 1& "# ! # 0 in vitro, / !#/# / ! 0 / + -0 – !" ## ) , &!+ .!# . /- [92, 95]. $! / 3 • .!# # . !! 1 " !+ " +!/ + !#/ # /%5 .! ! $# " - [88]: + H2O2 → • H + OH– + O2, – 2 $! ! 0 ! 0 /% ) in vivo) .! • / • %*"-&!+* !" 3 !/ - ( - #1" [92]. *+& 8**! / ), !4! . !! 8**! / -6!##1 #1 ! ! $#1 +!/ (2) #!" ! 0 " ( ! 0 + , / ! /%- !# - Men+: Fe2+, Cu+, Ti3+, # 39!" /! !# Cr2+, Co2+) [82, 183]: Men+ + H2O2 → Me (n+1)+ + • H + OH– Me (n+1)+ + • – 2 C Men+ + O2 ROOH + Men+ + H+ & %*+ , + & +! +!+. #1 [92]. &# /% ! 2 2 2 / 6! #! !+- ! 0 , &/ .# /% , -3 # ! + !.! /% &! !3 5 &! !$% 2 2 !/ &#1! / !/ / !## , / / / -+ - / !+ . $ !/ ! %+ + !- . " - [92, 95, 180]. NOO– [84, 169], . 3 54 " - %*$* &* (5) . $# $ ** #$ ## !##1 8 ! / # . /% ) (4) Me(n+1)+ + H2O + RO•, .!# 2 ! %+ #! 1 2 .# /%5 # 0 & 2 (3) • – - $1 ( # / #/ - 3 + $!" / 2 $ + 3 / NO• [108]: • – 2 # + ! 0 ## / ! 0 104–108 1 " /%5 - # 0 0 3 &! + &! + NO• → NOO–, .# / –1 # ⋅ ! –1 (6) • !, /% &! !3 ) .1 . / ) [84, 103]. -!/ & ! /• , ! # / / .!# $#1! +!+. #1 [63, 121]. !$ 6!# . %9 ! (/ . . 4). 39 &! / 3 &#1 * - . .1 # 0 0 / ! ! # , !# - + $! %#1 /!+ ) # - ( 0 0 ++!#/ /!+ " 0 4 in vitro /! / ) *0*/ % * %2 % '( *$*1*!1** !+ ! / # ! / 3$!"- ! .1&# 54!! 3 - &!# ! .1&# !/ ! 3- &!# ! 8 !# ! [37] . - [167] #!#/ * / - [2, 181] ). ! B / " $ +!#/ %3 !/ - + &! / %3 !/ - / % , &! %/ * !/ - #/ ## ! ) ( # 3 + / + /!+1 # 0 0 *4*/ % * %2 % '( *$*1*!1** !#/ # (Fe2+ ! / + 2 2; Cu2+ + 2 #1+ . 3 + • H ( +. . 3 !/ - , 3$. 1.1). $ # 3 # . !! 9 2) 3 !+1 Cu2+ B 4! # 0 / !, ) Fe3+ + . / #1 $ , +!/ / - Fe – ! $ #/ + . !/ / 3 !$ #!# - ) %3 [25, 142] #1 ! ! $# " . - [35, 57, !#/- 82, 105] !# ! ; . 8/ " #1 # / 8-, $ /! + &! %3 $ 0 5 &! / ! ! / .1/% # %3 !/ - $ - # 0 0 + ADP ! 137] %* / +!$ (II). # / .B / ) +!#/ . .1&# +! / 6! !3 + , / .1/% / + ! 3+1 %3 !/ - %- . +!#-!/ - 8 /! # Fe2+ [82, 99, / # + # - [56, 92] - #. 3 6!# /%5 . 3 5/ - ! 40 /!+! + , / / -## " - $ 1. [21, 92, 175] / /!+ ) # - ( 0 0 !, $ - ) 3 6!# $1 . $#1! #/ # $ 3 + / $1 . / / – 2 2 , !/ – # -• 2 - !# ! NADPH ( - +! % • 2 NADH + *!# 3 #+!/ - – 2, 2 %* / ( ) + # /- # " /!/ 3 # 3 / # $ $!" / * + 3 & 4! /! / 1 !, !" Cu2+ (& / — 3 , &/ / / . [99, 114, ($ 119, 133] #.- # . 3 - . /#1 /#1 • – 2 (0 / $ 0 - . • – ! . /% !+ • – 2 / # . [58, 143, - #1" / # 195] !# !## /% • / # !$ – 2 # - . # 0 0 5) !#/ # (Fe2+ + H2O2), # !+ ! / H2O2), $ 3 [167] . 3 !/ - 2 !# - + ). !+!##1 %3 .- !# $ . - + .# 4! / -5/ 1-, Fe2+. $ # 6 !/ ) 2 ! + 4! / !## 8 .# " # . 0 " / 1 + + - $! !# !+ + 6!/ .1/% # 0 0 -) ) ) = . #/ #, 0!/ %$!, $) . 3 !/- ( 5+ 3 #) — 450-3 189] # ! ! !# !+ 50 ,!# # # ! %3 - #0!#/ 0 !# ) $ 0 ! +!#/ / !, / # .) . / / / / [136, 188, 6$ !/ - # 6!# ! 2 $ . 3 5/ - / !/ / !## ). ) 19!# ! 2 0!#/ 0 / / $ -. / 2-8 ! / ##1+ • # /! / ! 1. B 4! ! !# ! . / / , $ $ 5/ - , $ ( " ) , # &! ! ++!#/ !$ #!# ", #/ # - $ 31 [92, 95, 162]. / $ +!/ # / + ! # / %3 ) # - $ 3 !$ #!# ", 2,2D- 3 . -(2- + $ # 41 # # ), $ +! 2,2D- 3 . -(2,4$ , - -!/ - - / -## - /% [92]. $# #! $ 0 + &! $#1 +- #/ # # 0 / . (• $ /! %#1+ $ /! %# " # . 0 !# / # ! $ / +!6$ Fe2+ H2O2, # - 76 –1 # ! / %3 # / #/ / !$ ) 6 / / !/ % –1 !/, /!+ #! +!#!!, $ , " / #1, .#1 1 +!$% !"& % +#!# ! 3 - # % / , .! # 0 0 5 !/ # / / , + ) ! "#1 3 %#1, $ + , /- !/ !$# ! #!- [149]. -3 ## " ## $# " / .! /1, + / !/ - 3+ 6#1" ! /! / !##1" / + -/ .!## 6# / , - &! #1, + +!$ (I) , 3 42 .! !$ #1 +!$ #! / 1 ! . Cu2+, 1 5& - ! /! / !##1! +!/ . + !- # 0 - -54 ! . # $! $! & / - +!$ [54, 55, 67, 111, 161]: $ / - 1/ . !" Cu2+ !##1" ! / / -# !$ - , !##1 ex tempore, / #1 6 -$ / , / 3+ 6# / % $ ##1!, &#1! !4! / , . 6$ /% ! 0 , / /! %# , # / . $# + (#! -3 ## + $ - # 0 0 !" Cu2+ / . 1 #- . / / . 3 + $!" / / !- ) %# /% Fe2+ # $# 6$1 , # 3+!. !" / !&!## /% Cu2+ #! / . [92]. /! !" 1 6 !/ + / %# + +!/ $ # 0 / !$!# %3 4! / !## - & /% +!$ vivo, ⋅ $ +!#-!/ - # / #/ [92], $ 31. ) 54 ! $ 0 $ 0 –1 #/ # /% -, !" 6! !3 (II), ! 0 # - + 6!/ .1/% B /% –1 6#1 /!+1 $ - +! ! 1 . / 6# / 19!: 4,7⋅103 3$ ⋅ , &! $ " $ /1, #/! *! # 6 /% - . * 3 # / /% - -!/ - # 3 - ) # ! $ ## " " /! !## /% ) 1: ! / %3 +! / , , . !$! !##1! !$ , /% . $!/ 3$ !/ H2O2 , 0 .! # 0 # 0 / !/ - , # &!# .! /!+! . $!/ !3 !$! -54 - , $ 0 [95], $# 2) 2 ) !$ / / + 8/ – 6#1 $ !#/ # (Fe2++H2O2) # . !! 9 ! / . , &! ! 0 2 .#1 , / ! . 3 54 ! - + , / . 3 1 /% - $ 3# " ) - $ 3* 3 $ + ) .! $! !# - " / $ 3# " ) ! + . ! 3 % + . 3 +, ) [55, 161]. in ) # 0 0 !# ) ) 3 5/ ! 3+1 %* / +!$ + & 4! % &! !, +1 /# &!+ Cu2+ -$ /! %# . !! ) #! / "& 3 8**! / -5/ + / # % # ! + , 1 - !# 5, &!+ # #, #/ Cu2+, /% #1! # 0 / 1 ) !$# 3# &!##1 $ - .!## , $ .#1! $ - - %3 # - $ #/ . $+* !1*- *$ $%*,$ % * 2& 1 %% , %, ' +$ &!+* !"#$ 9 1.3.4. %- /#1 [120]. !$!##1! 19! $ ##1! , & /# / , Cu2+ .1&# / &# $ 5+ , #0!#/ 0 #/ /#1! +!+. #1 / #!" 6 /!+! Cu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• $ / #- 8 ! / , 0!# # / 5 3+ 6# /% 1- !# - 2 %3 +!#-5/ ;) - ! / !+ %#1 $ $ 102, 124, 168, 179] – (• $ +! , / %#1 (#! $ $ # + &! %# + 6# 9 / 3 & 54!, 5/ - !$ #!# - [8, 159]. %3 /! %# /% +!/ $ , # + #!#/ # # ! 8/ + / &! " , / -4!, $! !## " /!+1 # +!/ $1 + #/! $! ) / +! / &! .1 $ - 9 - #/!# +!/ $1, . # 6 1 $ + + ! &#1 +!54 !$ #!# " ! ! / ! !- +-# /1! ! +!#/ . -5/ 1- # /%, 4!# ) # !# - , / ! /% /! 3 0 6 %#1 [65, 69, 91], 3 - + , # 3- $ / / 6! #1! / 1 +1 !+!##1! # 3 # #1 , / &! $ / #!# . # %#1 %#1! !$ #!# - . !! / . - $ / + /! in vitro (/ . . 5). in vitro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in vitro. , $ !# ) ($!" / 54 ! , - -5/ - -# ! !4! / # # !$ / -!/ #! +#!##1" #/! ! # &! , +!#!# -. 44 +! / , , #0!#/ 0 !# ! $! , $ + ) !$- . # %#1! , # 3+! $# -$ $! ) # 1 #!, . !$! !# ! #! $ ) $ /1 ) !# 14!##1! 6 %#1 $ / ! ! # , !# - !/ $ * #1! = , ;= ++!#/ A +!#-!/ - +!/ /1 ( +!#%9!# ! $ + , ,$ !$ + $! %#1 /!+ " /! / # - ) . 3 !/ - 9 " 0 5 , / + ! / ## , $ / [91] !# -. $! 6 # -) $ $ ! $1 - !. !/ - - ( ) ) #1" /! %# - 8 / -5/ I–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–285 #+ $ - / . [68, 71, 164, #!$ / / ! 2 ! - , 4!# - (220–240 #+ !# ). .1&# / !. !/ - !$ [32, 92, 109, 148, 175] - ) $ /1 ) !/ $ * ++!#/ /! %# - 8 / 0 - / " +!/ $ # , # &! 3 .B !, # + , / .1/% ! / / 3 / #1 $ # " /! %#1" + , ! $ $1 / 4!##1! . 3# &!# - +!/ $ : + / , * -; * -; - *! +!#/ / – –, 3 - #1" +!/ $ # 3 ; - ;= %/ * –* . / - $ /1 ) in vitro. B / ,$ $ , ! &! / [65, 116] - $ /1 . # # + . 3 5/ - !#/ # [65, 91, 115, . 1$! !# !+ , ! $ / / 54 - 3 ! 0 ## " $ !$! 3 / / / # 131] +!- . – 1 8**! / –+ - ! / +!/ + / , * -; ! / * / +!/ $#1 in vivo !# - ) = , 3 0 - ) 3 $#1 !$ #!# ", . 3 54 !4! #! . 3 5/ - 3# & +1 #0!#/ 0 3 . 0! ! ) #!&#1! /! %# !0 * &# /% #! 1 - !+1! $ !#1» 3 ! +-, , # #1! /! -+ . /# +!#!# !+ # $ .#1 /1 8/ #. ! + * / $ / + ) / , #$ #- /! / /! %# /% 19!#1 #! - -5/ - ;= ) / # , ! «8 / , . ) + " +!/ !/ " . -. / # - 6 $ -; ! / ; /# - –/ / + / , * -; = +!/ – * / +!/ -; - – , 3 6 $ –/ # $ + " . "# / /# - + / , - ! / ; ! +– ) 1. ! # # !/# , +!/ $ $ / -. #1, $ / &! # * +!# + /%5 +!/ &! !0 * &# /%5, &!+ !$ / ! !" 4! / # # # " #1 / /1 , &! "#1 / &! ! ) # -+ %#1 1 1/ , 3 & / # - !$ - !$ - 8/ + / - , / !. /% + # - 3+ 6# /% $# / !/ / 54 " # - /! %# /%5 #0!#/ 0 / . # 9!" !3 %/ / /! %# /%5 3 3 +# + & - ) %3 +!/ &! 1 +!/ $ / .! $ #! !0 * &! " / "( !$ #!# -+ ( %$!, $1 ) # !+ . %9 / &! ! / # " . 3 49 !$! !- ;= + - /1 3 / / - !+!# # ! "- #- ) . !$! !# ! !- - 2 ). ;/ / +!/ $, $# - 2 , /- . 3 !/ - 0!$ 1 [70, 72]. . # .!# ! , ). - . ;/ , %3 !/ - !4! / !/ #1) +!/ !$!# - + 9/ .#1 4! / !## - & /% + " # , $ 3 #( $ / !+ !+ $ - 0!# !0 * &# /%5, $! # - # . !! 9 . . / -5/ ! +!/ $1 $ / / &# 5/ - $ - /! %# /% !/ !0 * &# /%5, &!+ $ / / $ %#1 #!# !, !, 54 ! - 2,4-$ # / *!# + $# !0 * &# /%5, $ / / &# " $ - .# - . & !+ " $# + 3 -$ 6# , & !/ - #!$ / / &# " 3 /! %# /%5 -, /% - / +!/- &# , /! / .! !& & - +-# / " , 4! / , ! , 3 $ / / &# " /! !# $ / , #! / !. 5/ # $, / / - ! / +!#!# $ / ! #1 !+ #! +!#!!, . #!# $ , ". # / ) $ .!# " $# " / !$!# ! . %9 , & . 30 . #- ) !$! !# - $ ! / #1 / !$!# ! # . $ !/ . !! # 3 " & #$ ., + , 5/ / -#- 1/ . ! +!/ $1 # ! # !$ ) /! %# /%5, & 5/ - +!#%9!" & #/!# &!# - / / / &! !$!##1+ / !. +!/ $ "#1 . 0!# / 1! 3+!#!# " / # /!" ! 3 # / . " +!/ $ $ 6!# . $ /% 1 $ + /%5 $ - / / 3 . %9 , & 6!# - + 1 &! $# , &/ !+!# # # & $ / 3$ . !! $ .#1 $ - ! $ #/# " , %#1 ! +!/ $1 1/ , $ 6!# .1/% + %#1 3 / / !+!## , # + / , * &! # #, ! . # ! +!/ $1 / & ! #/ / ! 9!## 3 /% +!6$ / &# /%5 # /%5 ! # # !+ %# " , /% / !/ / 54 " - -!/. # %#1- , $ 3 - # , / 1! + + 4! &# " !$! , 4!# - 3$ , $ 9 !$! !# ! + [164] ) . # $! !# - . # %#1! 3$! - * / +!/ &! + / # #1! $ - ;= - . # $ - + &! " [178] , $ 3 # /! / ! $! 6 # - 0 / #1 6 "# " ) 54!, /!+ . !$ 1 - +!#!# $ - !- + , / .1/% / 6! + / , * . # $ - /#1 [68, 165]. +! ++1 / 3 $ / ! + . $# /! %# #!$ # . %#1 $!#/ * / # % # "/ %#1 , [73, 152]. $ $ .1 !, $#-9# " $!#% #!/ + &! # ! !$ #!# ", / 1 + , / .1/% +!#!# - !&!# . !3#% ! $0 , #!" $!,!#! / # + $ .!/!, . %3 %#1 [70]. # $ / ) / %# / . +. + . 3 +, # # 1 . # &! / ! $! $! $ # / *!# !$! !# - + # #1, # +!#-/% /# /% ) # !+ , 3 &! / !## , /% $! 6 # - / ##1" $ / !+ #! +!#!!, # + #! $ $ - % !$ #!# ". %3 / 3 $#1 $ /1 ) 3$! !#1 + , / - 3 #! 410–500 #+: !6$!# - .! %3 %#1 %#1 9!##1! $ / - $ !$! !# - !$ 6 %#1 # #1 600–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harmaceuticals, Inc. ( A ); , / . / # -); , / / +!/ *!# ), # ( / # - 0!/ / / α-L- !0 / # 3 $ / -/ / / +!/ #, $ $, # / # / - -. / -4- (2,6-$ - - 3 $ / $ &! / 3 $ / Reanal ( !#, /, # / / 3 $ / ! $ (3%-#1" -); $ # )8/ # #! 3 $ / - #-1- « ! +» ( $#1" / -) ( , 3 $ . /! !$!# # / -4!" -!#/ / -), * * # 0 #/ .&. / "* - % ! / * - # (10 % ! - -# - 3 $ / "#1 - - %* / * #1 243 #+); - &#1" * * / $ % %+ / ), $ 3 #, %* # / # / ) Merck %* # 0 # /, -) ( #0!#/ 0 - H2O2 ( $ - (2,3-$ +! " * . 8/ # !) . . / # ICN Koch-Light ( ! Sigma ( A ); N-(2,4-$ # / *!# (85 %), 8/ # + 0! / &! 3 $ / Serva ( ! + # -); 2-(N-+ * - , $ (36,5 %), / &.$. .); 3 $ / / /!/ , $ /, +!$ (II) 0!/ / + # , $ /, +!$ (II) , $ /, # / / . !## - * + , GSH), , $ / 3 $ / (MES) # / %$!, $ ! 0!/ # $ , $ /, 2-/ ( ! + # -); # / / 1" 1: / / # ). ( ' & + ,!$* ) $1 $, / . ! / # , # 1607 (LKB, A !0 -); # -); -80 ( & , !/ ) -3 / 1 ( 8+ , . / &! ! ! 1 ! / * / +!/ -46 ( 5 !/# ! /$! !# ! ($ +!/ ) -8 ( -); # +! / ! 1) Pipetman (Gilson, 3 . 6!# - / /! %# /!" / &! ! 0 " . /1 .1 $ %3 # -: +!9 + ,# /# - + $! % -) , -+!/ -130 ( * + + &! 3$ % %3 . -); !$ #!# " # !+ Sketch 4.55 (Advances Chemistry Development Inc., 3$ 9#1" $ * ,+ " # $# " $! / #1! 0!#/ / + / &! * - !$ 3 - # -#$ -). Finnpipette (Labsystems, / #1 52 !$ 54 ! -21 () %9 ); /! + / / / ! / - 1 ++); #0 -) #1 !+ !$ - , ++1 Chem- # $ ; http://www.acdlabs.com). &*9 ," $* "*2 % 4 +1 $ . + [46]. *! # , ! 0,25 + #!&# - #0!#/ 0 - * * %#1! # " * 3! ! !$ * - / , / !# !+ * * $1 * * ##1+ / !/ / 54!, . # 9 0 + $ #! . $ + " $ / # " #0!#/ - !#3 + . %- $ . - $ # 6# " #0!#/ 0 $- $ . - $ # 6# " #0!#/ 0 8/ - +. !$ !+1! !4! / / #6! 0 2,38 +,/+ . !$ !+1! !4! / %#1! # %# + / , / # + ! !+!9 1 +!#/ /!+ ## " / -## + #/!# 8/ # !. $ * + $! %#1 5+ $ / / 9 # / !8 # - - 8/ , , " .1 / 0 %3 $ ! !$ , / !# !+ +. *%" $* "*2 % 4 +1 $ ! , #/ ## + / ! 37 . - #$ 0 0!#/ 0 ) #1 /! /! 2& + " $* #$ 0 !# $ - ## + !# 5 + ( !4! / %3 / !/ / 54 + # + /!+ ! ##1! / . #- 0 +. $1 $ &!1** !&' $*"#$(7 % +*$ $*8 % *%2 "#0 ,!$* 4 N-(2,4- +*$* & : $*")9*+&!0*$! #0!#/ 0 5 . # %#1 , # %#1+ +!/ $ +. - 8/ , / ! 0 . B! !3 10 + # 10 + # / &! / 5 !#3 , / / / !# - ! / ## " +! . # $ . / - $ / !. ! / , / #/ $ $ 3 30–40 + # $ 53 - !$! - - 0,2 + ! - NaOH. 4! &! !3 $ %# " #! #1 460 #+ .1 ( $ ). ), $ 3 # ( &! / !#3 ## 5 1 + 0,75 3+! - N-(2,4-$ # / *!# / !. !+ ! +$ . - ## " +! ## " HCl. B! !3 30–60 ! + !#3 / !/ / 54 + . 3 + . . / ## " - HCl 0,05 + /# /% ! 0 . 5+ 9 !$ #!# " ) !#$ +!$ !## HCl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– 2- # 0,2 + , #1 232 #+ / * / +!/ 8/ # - (1:1) !# - * 3 $ . " * 31 /. !$! - /. 0,05 + - /!+ $ - . !$ 5- #$ !! " 1800×g. ##1 $ !# # + /1 $ . 10 + # #1 535 #+ 532 #+ /!&!# ! 1 & # $6;9*& ,!$$(7 +* $ , * & !#3 /!&!# ! 1 + # 0,2 + #! 3000 g, # 452 2 0,6 % ## " +! * , /1 ( 2 ), !#3 ## 5 +! % # . ! 0 0,5 + .1 ( $ ). 0,15 + /1, 0,5 + 6$!# - # +!/ $ ! FeSO4. - 10 + # %# " $ - " * * # " +-1 1% * * # " FeSO4⋅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– C1) / C0 ] × 100 %, 55 ! + &! $ , !# - !$ #!+ ,$! #0!#/ 0 - – 0 4!" !$ !+ , # " . # %#1 !$ #!# - ( #/ !#3 +, 3# &!# ! 3 /! - 8**! / , &/ /! / ! 3 /! - 8**! / 3# &!# ! ! *% */ % * 4 ! 3 ## , # / &#1 , $ #/# , $!" / #/ !$ / 1/ , 0 1& ) #1+ ); - 1- !# - - .1 / 0 !. ! +!#$ - $! M ± SD, ,$! M – - #!# !. & #1 p /!31 $ + 4%5 + 4%5 $ - & ! 31 !/ ! - !# / ! ,!#! 3 + %#1 # !$# 3 1 ). ) 3# - 3# & +1+ ; 0,01 < p < 0,05 — -3 +!6$ $ +- !-# -; &!/1 3 /! -+ &! / !## 5 +! ( #!"# / 3 #!"# " !, ! ! & " +!6$ 8/ + , !$- / ! # /% / / # !##1 +!/ $ - 0!# #!# ! ! / / / &! !; +# - p > 0,05 — #!3# & +1+ . + 4%5 & # r). $ #/# ! $!" / / !/ / !3 %/ /1 / / / &! / !# ! $ , ++ !$! - - $ #/# ! $!" / $ 0,001 < p < 0,01 — 3# & +1+ ($ / ! #1+ ); 3 - .1 $ #/# , $!" / - -!/ 3 ANOVA (3# &!# ! ! - 0!# &# , !$ #!- [12, 39]. p < 0,001 +1 /! %#1+ ( %#1 1/). - -!/ #/ !, SD – / #$ /# ! / # $! 6 - . # !$ #!# ! ( #/ !3 %/ / -/# /% $ / ! # / # ! " , &!# # / . . / ##1! $ ##1! *+!/ &! !$# + !$ !+ ! +, #! +( 0 -+ , 3 6!##1+ #!! #0!#/ 0 - – !+ ! !4! / 5 . . / / / / &! 1 !#3 !+ ! !4! / , &/ /! / / / / &! %), $! 6 4!" 6 /! %#1+, & / /! %#1+,. & / !$ #!# " &!/ /! !# 8** 0 !#/ + / $# , 3 + #!"# " %-3 ! -- 3 /! - / $ , , . $ +!#!# !+ , ++ Statistica for Windows, Kernel Release 5.5 A * 56 !/ $#1 +1 StatSoft, Inc. ( A ). - "!,! 3. !0&!' !$ * ! * 2 *4*0!1*- 2&*4 $ $*- 4 + "#$ 8 %*% 4( +"- 1 $ * %*+!$ $ 9 + 8% ,*- , 5 % , $! %$ , "*2 % 4 3.1. &!,$* "#$!- 1 $ ! 2&*4 $*4 % * 4 2& + , + , 2& + " $*- !&' $*"#$(7 % *%2 "#0 ,!$* 4 * '!&'* & , 8 *%" ( * 2,4-+*$* & - : $*"9*+&!0*$! , "*2 % 4!"#$ 8 4 + "#$ 8 %*% 4 &*4 $*4 % # 4 3.1.1. * '!&'* +! 2& + " $*- , 5 % ,, & !9*& ;5*7 % & , 8 *%" 8( ), +"- 1 $ * & ,$- - , 4 + "#$ 8 %*% 4 . . / !$ #!# ", - 5& - , 4!# - / ( 2 ) . # $ 5 &! !$% 3 + [70]. + ,!#1, 3 54 # !+ # $! , &# + , ) / + / Fe2+ -# ! !#3 /1. 54!" $ %$!, $ ( ) # +, Fe2+ #0!#/ 0 #! !/ ) / , +!/ Fe2+ $ ! ,# /1 Cu2+ $! # &! / 3 !/ - 8" ! - &! / . -3 # - 2 3 6!- # + Fe2+ [37, 65, !#/# / + , / # # 0 0 !$! !# - / !# 5, %* / .1 / / 3-/1 ) 57 !$ #!# " - . !3 %/ /1 !4! / 2,5 + + + + #1" [70, 6! !3 [92]. ;/ %3 5/ - Cu2+ # +#!# 5 !# !". - / - #1 532 #+, . 3 5/ - ! ! $# " !+ " ! 0 ! / + + ,!#1 + #1 452 #+, ! + $ / !##1 !+ 4! / !## , Cu2+ 9 8/ #! / / 3 #1 $ , $ % 3 & , 4 54 ! $ # $ / $ + + , -/% # & 9 + + . !! #/!# # - , ! -/# , $ , ! +!6 / &#1 69]. ) - "+ , 4 54 ! + ,!# /% 532 #+; / , $ $-/ 3 ,! - . 3 # ! , !3 %/ /! . 3 5/ - # 452 + ,!#1, ! /. [66], %#1!. # 71]. & / !/ -, &/ .# . ) , +1 $! 6 # / #0!#/ 0 - 2 # " , / !/ /- # 2. ) , 4!# ! 3 54 $ /+ " /1 ( 0,625 + Fe2+; 3 – 0,625 + Cu2+. 9 8 - 2 , !$ #!# ", # # 452 #+ ( ) 3 + $!" / (2,38 +, / $ !#3 . # $ ! ,# /1 ), / / / 1). 1 – / / +!#/1 !$! !## ! !$! !##1" 3 , &/ / / / / / / ! ! Fe2+ # +!#/ / Cu2+, .1 ! !" +!/ 58 !#3 !# 5 Cu2+; 4 – 48 + ,!# , . - !$ #!# " / ! 0,625 + !$#!# ! !3 %/ / ! %#1 532 #+ ( ) 37 +!/ , + / . . / / ;2– / / 0,625 + Fe2+ (M ± SD). !##1 19!, &!+ ! ! $# " + $! 6 # ! !#% 8/ !#/# / - . 2). ) ( 8/ + # ! . 3 . , # ! $! 6 4 CuSO4, # -. , 0 !/ , $ Cu2O. ;/ # . 5$!# ! + 6!/ $!/! % / $! # 3 # . 5$ /! # , $ - /% / # % #/!#$ +!$ (I) !# $! # 3 3 - &! / Cu2+ $ CuI. +!/#1 #!! 2 , $ N,ND-$ 8/ /. [50] . . / $!, $ 0 " . 3 , 4 54 ! ) #! $ . # ! 3# & /! %# , $ / 3 + $!" / ## , & ! . 3 !/ - / % &! / ! !+ FeIII $ $!" / + ,!# . / 1 # - / / &! !$ 6 + ,!#1, . 3 54 ! - 3 ! 0 CuSO4 ## " +! " , &/ %$!, $ , 4 54 $ +1 # . 5$ #! + ,!#, # 3 / , &#1" /!31 131 !/ * / . 3 !$ 6!##1" 2 , $ + / !. !/ & / - .1 !$ CuII. # - . $# 6 /%, &/ / # /. [50], +#!# - %# , $ /. [50] +! # 3+ . 3 !## ", / - + ,!# ## + 2 , $ + !/ #! FeIII, 1 / . 3 #1 452 #+ !# !+ Cu2+ $ CuI , + 6# .1 # 9!+ - ,! $ . !# / # / # #1 452 #+ &! / ! %# , &/ $ ! , !/ - . , , &# 5 % # / 8/ " , / " # !+ $ 2 , +!5/ # 3 %/ Cu2O, # - / , !++ ,!# !## " * + +!/ ! ! $# " !#/# / . .# / Cu2+ !, $- 3 -/% -, +1 !$ 6 + 6!/ . 3 1 /% - ! /! %# 2 ) Cu2+, / 6! / / Cu2+ ( . 3). !$!##1 ! / + + + !# / / / $! 6 # /# /% ! 0 . !$ !# ! $ ! #/ ## !##1+ #/!# #1 452–453 #+ !#3 !##1 $ + #! 59 # ! / / / ! + ,!# + - 3 + $!" / . 3, 1). ) / / / ! Cu2+ !/ . 3 - 3 + $!"- +( #1 452 #+ .1 CuSO4 / + #1 452 #+, - $ / !##1 !, %#1! ( /# + + # ! . 3 2 2 . $ **! !#0 $ / , . 3 54 - 2 ## " +! # #! Cu2+ , 4!# - #! ## " +! ! 0 .# / , 4 54 " 6!# - +1 $# , &/ , 4!# - # $ Cu2+ $ 6! + ,!#, /% - !$ / !## + 3 + $!" / ! / 1 2 3 2 #! $ ## , / / , &/ / # / &! &!#% # 3 " ( # ! !$!- . 3, 2). $! # 3 . %9 , &! / $! 6 # ! # ! / 1 " (2,38 +, + , 4 54 $ /1 ( 2 ), !#3 $ / !# -; 3 – 2 + $- 3 8/ - 19 !/ . 3, 3). 3 + $!" / * * / $ , , 4 54 + #! /! %# ! 18-& , !# - + 0,625 + + 452 #+, , - - Cu2+. 4! / !## - & /% . 3 54 ! - !##1 $ / + ) / / # 1 +1 , !+, &/ !#3 - 2 #1 532 #+ ( ) ! 18-& # . 5$!# " +1 # #! Cu2+; 2 – 2 + +1 3 + $!" / + ,!# , #/!# , 4!# - 452–454 #+ %* / +!$ (II) ( ! / 1 #-/1 /# ) 2 ): 1 – 2 + 0,625 + + $!" / + + + , 4!# - !4! / , . 3 54 $ /+ / / + ,!# , 3. . . / + ,!# 2 $ 0 Cu2+ / / !/ - #! - !3 %/ /! 3 - !$ / !## + 3 + $!" / 2 Cu2+. ) &!##1! # + .1/% $ / ! #1+ 0!, ! / #!+ #1 +!/ $ !3 %/ /1 3 /! !+ / / 5/ / ! ! $# " # /! %#1 !## 3 + $!" / / /1 !$! !# - $! 6 # - $ / #1 Fe2+ Cu2+, !#/# / &! / /% $!/! % / 5/, &/ - 2 , 2 . #0!#/ 0 / + ) $ / ) 60 # 5/ . 3 # # ! 4! / !## 3 !+ + . 3- $# " / #1, Cu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u2+- #$ 0 / / 3 3 ! /# , &/ , 4!# - $ # / *!# #/ ## + %#1! #! . $ + /%5 1. !$ #!# " +!5/ $ # + - &#1 6# 3+! !# -. % !$! ! . . / . # # # # 14!##1! 6 $! ! 0 " ) # -+ Cu2+ !# - $ / &! / !## , &#1+ 4! &# " $! 6 4!, / % + +1 / . 3 54 ! - * / , 3 &! %#1 ! / , &/ 5 #$ / $/ ! 6$ !/, &/ +!/ $ # !+ #1 $ - * / +!/ $ 3$!" / 9!##1 %. ;/ / * / / + 3 0 %3 3 # 9!##1 , . 3 5/ - $! ) # - !$!##1! 3 - &# , * * / $ # -α-L- !0 / # , 3 H2O2. + + # 5 #/!# &#! , 0 !/ . 3 ! 0 !##1+ . 3 # - !/ $ / + 3 + $!" / / & ! / 2 , +!#/1, #! ! , $, 0!/ /), / $ , ! / ), & / + + + . # 0 0 , 4!# ) - # ! / 4. , 4!# - !$ #!# ", . 3 54 $ /+ ), . !" Cu2+; 2 – #-/1 /# + /! %# , $ . # +!/ $ ## " %# " ! # 3 + $!" / * * / $ # 1 +1 ). !( ) / / !# !+ CuSO4; 3 – # . 0 #/ $ / , $ 3 # +( / / / 37 #0!#/ 0 - Cu2+ ! / $! ) 2,4-$ # / *!# 48 & # - - -* + !$! $ . / .1 ($ / 3 . 62 - . # %#1 + (2,38 +, !$ /! %# - # - H2O2 ( ): 1 – .!3 $ . !- !# !+ Cu(CH3COO)2. #!&# - 2,5 + , H2O2 – 0,8 + . ! / 1 ## - $ ), # . ## " ! / , 4!# - + + +!!/ + . %9 ! !& . # , 4!# . - + +1 445 #+. ! 1 6!## ! 3 !& , $ / 1. ! 8/ 3# !# $ / # #1! . !! / ) ! 6# / : %#1+ $! 440 #+, # 3 3 $ !4! / ( # #1! "( , $ 3 # 3 /! !" $ %#1! %#1 $ - $ /1 ) ! / , !$#!0! . # %#1! ! +!#/ %#1! , 3 #! 0,1–2 + 63 ( %#1+ / !+-, &!&#1! - 0!/ # $#1 / $ /1 ) 3 # $ # , $# " " — # 405–440 #+ [13, 24], / ! /% . + / &! . # . # +!!/ + /# / / 1) - -!/ . 5). [70, 72]. &#1+ . /1) - -5/ - / . !$ 6!##1+ # + ! ! &!/! # /! !#%5 $ / ! # / # #- #1 460 #+. , $ 3 #1, +!54 ! &! / ! 8/ # / *!# #! - ! 0 !/ #1 ( 0!/ #, 0!/ /! %#1 8 / + + /# / / - $ .) 3 #! $ !$ + /% !$! (8/ - # #!# - / " . # / # % !$ #!# -+ +1 1. !$!# - , . 3 !/ 4! &# " ++! &! ! / , &!+ / , ) , — - -5/ - $ / / &# #! / . #1 # %, ,! !$ #!# -+ . + + + 450–470 #+ $ / !$! !# - # #1! !# !$!# - ! $ +!/ $ ++! &! " . # , $# 5 # , . , 4!# - # + ! / 1 . - + + / &! $ , . 3 5/ $ # / *!# + + #!- . 3 5/ - ## , ) , $ 3 # + 4-, $ +1 #! $ / (. / # %, 4! &!" + + ,!#1 # #1 %#!"9 ! 3+! !# - , $ $! $ .), $ , !+, &/ # . 5$ 54 " - # + + 5/ 3 ! /#1! $ !# , 415 #+ $ , 4!# - 456 #+ $ # / *!# # $ - !$ #!# -+ [70, 72] # + + + !# !". ) # + $ / + ) ) 3 " + # #!# %, $ # 3 -3 # # " +! $! # +!5/ 9 !& $ + & - Cu2+- #$ 0 ) [72], +1 , 4!# - + 6!/ .1/% $ , ! !##1+ 410–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u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e2+ - 2 ( !/ $ 2), + / / #75, %$!, $, .!. ##1! / . * ** ) *** Fe2+ ##1! $ !#1, .!. / # 1" 0! / ! # /% / #$ 0 !$! !# ! $ ## ! ) ** !# -, & 3 24 3 24 141,70 907,56 560,26 1268,39 ± 49,31 ± 200,06* ± 110,95 ± 153,48* 5,360 23,425 *** *** ± 0,228 ± 0,871* 14,170 29,726 *** *** ± 0,199 ± 0,265* 5,598 9,145 *** *** ± 0,322 ± 0,301* 11,453 20,256 *** *** ± 0,380 ± 0,426* 0,009 0,048 0,074 0,135 ± 0,004 ± 0,008* ± 0,031 ± 0,034* 0,030 0,093 0,083 0,177 ± 0,009 ± 0,011* ± 0,014 ± 0,018* : !# - Fe2+ .!3 $ . $ ! ,# /1 : !# - Fe2+ .!3 $ . 0 6 . #$ 0 )! !$ #!# -, + , !# ! ) $ /1 ) %#1! $ ## + ) / . # + $! M ± SD. !$!#1 & " +!6$ $ . 3 /! -+ !# !+ 2,5 + 3 /! - #! $! !$#!# ! $ ##1 7 8 !3 24 & ) 0!/ / +!$ (II) /# ( +. 65 3$. 3.1.1) 0,8 + ! +!#/ . <0,01. H2O2. # 3+!#!# ! 6. (2,38 +, 2,5 + $ /+ ) 3 /! - / # 1" 0,8 + 3 &#1 24 & / . # %#1! , ! Fe2+ (5 ), / / Fe2+ 78 ! 5/, &/ $ # + 54 , &! , " # !# ! ,/ #$ 0 !# - $ , #-/1 +!/ $ . !$ #!# " + #/ ## , / 6! $ +!6$ #$ 0 ## + ) ) !+-, ) .1 , &! /- 24-+ & 3-+ ## - $ / . # %3 %#1 # !+ 3 + # . !! !$ #!# ", . 3 54 -!/ # . !! - /! / #1+ $ ##1+ [37, 66, 70, 72, 92, 191]. B %3 / &# $ - $ # + !# - . 6). &! / !/ - / $ $# # $! , /+!/ /%, &/ +!/ $ !$! -/% (M ± SD). +!#/ %#1 6# !$ #!# -, 3 + $!" / 54 ! / / / . # 1 6!##1+ ( ##1! $ !#1 (1); +!/ $ 1: # !+ .4! / #75, : / !/ / 5- Fe2+ %3 !# - ( #-/ 3# &!# ! ## , / / !$! -!+1 !# ! , #$ 0 ! Fe2+ (4 ), &! / !## # ) + / / / !# !## !#3 +!/ $ 2: !$! - 9 ! - !$ #!# ", ! , ) 100 % !# -. ) $ /1 ) !$!##1! $ ##1! $!+ # / %#1 $ / !# - ( ) H2O2 ( ). ! 3& !$#!# ! !3 %/ / ) 3 !$! - 9 ! - - 2 , (5 ). . # $ %$!, $ (2); - 2 , (4 ); ! Cu(CH3COO)2 4!, (3); $! 6 # - # !+ 6! 0,02 + (#! # #, 1 !$ !$! !# - $ / # " ) , , !$ 66 . # / !+-, %#1 , +!/ $1 /! %# 8 / , $! ) / # , &/ /! %# /% +!#! $ / - ) ! / * / +!/##1 , # &!- + / /! -+ # 3 5 & ( +. / ( #75, /! %# /% ##1! $ !#1 !0 * &# /% / # 1" %$!, $), $ / / &# / $ !+ 1 #!# 3$. 1.3.3). ;/ / * / . # %#1 ) 8 3 -!/ # + ! +!#$ $ / ! /% ) +!#/ %3 $ - $! # ! !$! !# - /# " 0!# #/!# $! 6 # - # / ) in vitro. 2 *4*0!1*- %" ,*8 2&*4 $ $*- 4 + "#$ 8 %*% 4( 3.2. "*2 % 4 % Cu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–).1.13). !# - !$ #!# " #0!#/ 0 - +!#/ #0!#/ 0 - 0,8 + ). !$# + $! 6 # - ! - /$! %# / &!##1! # + $! 8 !$ #!# " - $ 6 ) 19!, &!+ !$ 54 $ / + $ / 4! / !## !# - ! #0!#/ 0 3 %#1 +!#/1 +1 H2O2 ( #!&# - !$ / $! 6 # ! ! %5 $ + &! 3# & + / 21 . # ! %#1+ - #!&# " H2O2, / 6! / % !# - ! !# 5. +!$ (II) ($ !# 5. /! %# / . ;/ 8 !# # /1 # $# , &/ /!&!# ! 24 &, + 3-& 3 &!# ! $ # + 3 3$. 3.1.3, $ ! ,# /1 $ ! ,# /1 .1 /1 +!5/ 24-+ & !$ #!# " #0!#/ 0 #/1). !6$ 48 ! 4! /! 48- , /! / - + , $ ! # - 72 & ) $! - 6 # ! . # 0 5 / &! # 6!# 5, # 8/ #! 3+!#-!/ -, 96-+ & # 6!# ! / / / &! $ 6! - -!/ /!#$!#- #!3# & + ( +. ) 6!# ! 1). . $! 6 # ! . # %#1 !$ #!# " (+ ) #/ ## + )! #$ 0 !#3 ## + $ !# 5 #$ 0 - ) !- +, 0 7 $ ! ,# /1 37 * A HP CS CSHP CA CAHP 0,113 0,169 0,107 0,169 0,157 0,308 ± 0,038 ± 0,047 ± 0,028 ± 0,054 ± 0,056 ± 0,109 0,141 0,219 0,175 0,270 0,300 0,560 ± 0,049 ± 0,057 ± 0,043 ± 0,103 ± 0,072 ± 0,111 0,189 0,357 0,303 0,417 0,560 0,777 ± 0,065 ± 0,079 ± 0,113 ± 0,186 ± 0,103 ± 0,106 0,908 0,988 1,214 1,270 1,238 1,268 ± 0,200 ± 0,177 ± 0,141 ± 0,139 ± 0,148 ± 0,154 1,228 1,184 1,387 1,392 1,259 1,298 ± 0,296 ± 0,164 ± 0,160 ± 0,158 ± 0,150 ± 0,246 1,486 1,437 1,386 1,376 1,201 1,160 ± 0,061 ± 0,028 ± 0,044 ± 0,048 ± 0,033 ± 0,101 1,296 1,422 1,373 1,373 1,212 0,961 ± 0,209 ± 0,072 ± 0,032 ± 0,022 ± 0,039 ± 0,165 +!#/ +) !$!#1 $! M ± SD. # -, & 1 3 6 24 48 72 96 ##1! ( !$#!# ! / / / &! 15–22 8 " 3# & + / #1! . 3# &!# - * 3 $ ! & " +!6$ , #$ 0 : + !$!#1 ) / – !# !; # 6!# 1. – H2O2; CS – CuSO4; CSHP – CuSO4 + H2O2; CA – Cu(CH3COO)2; CAHP – Cu(CH3COO)2 + H2O2. #!&# . # %#1 #0!#/ 0 - H2O2 !# ! # . 0 !$ #!# " # . 0 ## " ! 1! & 1 ## " !$! H2O2 #! 0! ) 68 !$! 0,8 + , % ( / / / &! !" Cu2+ – 2,5 + . -!/ # !# ! 3# & +1! / . & -, p<0,01), $# $! 6 # ! . # (p>0,2) ( + / &!##1 8/ %#1 ! 24 & !#3 - ## + +, !# 5, 3# & + !# $ ! ,# /1 #! / & + $ , 36!) - / - / $ , . 7, ). 7. 37 3+!#!# ! / / / 0!/ / ( ) +!$ (II). 1 – /! !# - ( !$ #!# " H2O2- #$ 0 !# 5 # # 0!# 9 . ! $! 6 # ! . # $! $ !" +!$ ( ), / / / !3!#/ / $ . ! H2O2; 2 – # / !# $ . /! %# , !# - 2,5 + %* / ( ) !# !$!#1 / . . 7. 69 0,8 + H2O2. ) 3 - #$ 0 19!# 5 # - (6 !# #- +$ . . # %#1 !# !+ CuSO4 $ / ) $ / CuSO4 #! / 3 & # . 0 CuSO4, $# 3 ##1! 3 /! %# / #$ 0 ) #$ 0 . # # !# 5 / #$ 0 !$ !/ /+!/ /%, &/ ! !##1 0 0 ) ). ) - !$ #!# " .7- #!# !, &!+ 3+ 6# ": (1) !# ! #! . !! &!+ $ ! , 5/ /1, / / +!# #$ / # # & %#1 8/ & # . 0 ) + 3 &#1+ #$ 0 # !+ +( 3 6& 70 / +, &/ - 8/ + 3+ 6# $ - ! . # +! # . # %#1 #1 3 2 $ / /! - /; (2) !# 5 / / ! # " - -3%. & + / . %#1 . / / . $ / / / / &! 3 !# 5 ) +# /! %#1 &! !3 1 & !/ - ! 24 48 & + !#% $ !# -, $# #! / -+ +!$ (p>0,12). 2 !! / , , 0!/ / + +!$ . # /% !# -, p<0,0004). . # (/ . . 7, $! 6 # - CuSO4 # Cu(CH3COO)2 3# & +1 &! !3 3 $! 6 # ! . 7, 9% #!3# & /! %# - & /% !# - 1 1,4 + - $# #!# 14!## 5 . 7, ). # - #1 9 !# !+ H2O2, % &! !3 48–72 & / + +!#/ #1 #!# 14!##1 6 ) $ . & - !# ! $!/! % / $ / . 3 ) = $! 6 4!" / % p=0,034, !, 19!, &!+ & - $ %#!"9!! # / # - + 6!/ #0!#/ 0 - . 3 # 6! /! !/ &! !/ 4 # !# !# ! + !+-, CuSO4 + H2O2- 3# & +1! / . # $ .# - $ # + $ / / / / &! !##1 ! / 6! ,$ / ! # !# - ( #0!#/ 0 + + +!#/ 24 & ! + $ / 6!# $ + + , + $ ! ,# /1 !# ! !& / % . # +, CuSO4 + H2O2 / / / 5/ . # #$ 0 !" ) $! 6 # + (p<0,003). . # CuSO4 $ 0 & - /!&!# ! 1, 3, 6 !##1 !# # # & %- +# /! %#1 (0,016<p<0,042). ) !#3 ## + !#3 #!# &!/ # ! . . # CuSO4 + H2O2 #!$ / ! #1 (p>0,175). CuSO4 #0!#/ 0 - 3 ) !# - ) !- &!##1 8/ & - / / / &! !# - $ / ! # + !$# !# 5 # !# - (1 . %9!" $ / + #1 8/ , / % 24 &) (p<0,006), # #! # # & %#1 H2O2 $ / /! %# + #- . # !# # . !! # 3 + / #! (1,2–1,3 + ) ( & - / / / &! ) ) #!# 5 !#% ! 1! 6 & ! !& 6! !##1+ #!# 6& !# -. ) . # / . Cu(CH3COO)2, !# /! %#1 . # %#1 $ . !# !+ 4! / !## 19! !# - (24 & #!, &/ $! 6 # - . !!) # 0 0 ) # 6!# 5 ( / . # !# - ) .1 - -!/ /!#$!#0 5 /! %# !# ! + # . !! #/!#- & - $ / ! #1, +. # 0 0 !/ - # / + 6! 96-+ & .1 & -+ ( / . # + . 3 +, # 0 0 1 6!## ! # 19! . !! $ 3 +# /! %#1, p=0,0365, /# # . 0 Cu(CH3COO)2 $! 6 # ! Cu(CH3COO)2 + H2O2 ! 1, 3 /! %# + /!+ Cu(CH3COO)2 + H2O2, / # - %3 + 6!# ! 1). / % +1 . # #1+ . !! $ 3# & +1, p<0,001). $ - # 0 0 !# ! + 72-& ! 72 & & - - !# -). + 0!/ / + +!$ (II) 131 !/ . !! $ / ) , &!+ # 0 0 - $ # !# - CuSO4. 3 ! !$ & - 6!# -, &/ $ #/#1 !# ! /! %#1 1/ * + +1 .# 3 # !+ !, 0!/ / + # / - #! 3 $#1! #0!#/ 0 &!# ! 4! / !##1+ , /! %# ! 6-& !# + 3# & + #! / ## " . # #/ , & . # %#1 $ / . 3 %#1 %#1 # 5 # . 0 !$ #!# " , 71 - ! CuSO4 $ / , ## " !- !/ - ! &!- $ $!" / !+ . 8). !# -. % // - + + !# - CH3COONa) .1 CH3COONa &#1 $-/ -). !$ #!# " $ 3 !$ + 3 + $!" / $ / % ) CuSO4+H2O2. ! !$! ( 6# 5 -# ! ($ ##1! #! - (.!3 $ . ! / / 3 & 8/ + 8**! / CH3COONa # . 0 $! 6 # - , !/ #0!#/ 0 - 0,2–5 + ) $! 6 # - !# - 1–24 &, CH3COONa /# & / / / / # 5 /! %# / ! , &/ 9!##1! $ / . # # # CuSO4 !# ! CH3COONa ( #!&# - . $ !# ! # 0 6 H2O2 54 $! -# # / 1 .1 Cu(CH3COO)2 !" Cu2+, +1 8**! / #0!#/ 0 " 0!/ / # / + , +!## $ , " / $! 6 # ! #/ !. . # #1, %#1 # . !! ! 24- 48- !$ #!# " # -# ! $ . 8. / 0 5 . # $ . %#1 $ / !# !+ 2,5 + CuSO4 0,8 + H2O2 ( ). + ! . 0 # / ;/ / * / !, -+ +!$ (II) !$ #!# " ! $ / !# / / !$ ## # H2O2 , $ # 1 / 1 ( # 9!+ .# /%5, &!+ , $ ,$! 1 / 5/ 0!/ / # / ## !# - ! CH3COONa # - ! (M ± SD). +!#/ ) + #0!#/ 0 !# - - . # %#1 !/ # / # ! / $ . . 3 !# %#1 - $ /- ) , $# $ %- $ / # - . # # . 0 ## 5 /! %# ! $! / !/ / 54 3 . # . # + , .# /%5 . 3 54!, - [82] 131 /% - $ !# ! ) 6!# ! 2). -3 # # !+ - $! 6 # ! . 9, ) 8/ $# , - !$ #!# " !# -, $ , + 3 & ! — +!/ / # ! # 0 . 7). 6 /%, &/ . 3 %#1 # 0 0 # #0!#- !+!## + $ . / / / &! /%, &/ + + ( - $ $# . # -# / 1 !$#!# ! $ ##1 4 8 CH3COONa + 6!/ .1/% !# ! !# - + # . 0 # . !! .1 / !! $ / , !/ + !$ #!# " # $ & !/ -, &/ + 0!/ / # / !( ) # -). 4 !/ - ( +. ) 6# # - !$ $! 6 # ! .7- # +, ! + . 3 +, / /- # / / / ! 24–48-+ & ## 5 + , # + 100 % !$! ( # . 0 ) / !/ / 54!, ## " #1 !# - %#1 72 $ # 0 / # Cu2+ ! . /% ! 0 ") 0!- # ! . $ 5/ +!#%9!" ! 0 + , / !,&! !$ ! 0 . %#1 %#1 . $ ## " - $# 5 * 3 , . # $ 0 / !+!## + $ . # 6!# ++ 3+!#!# !# %3 %#1 / / + # 0 # / ) . # ## + ) !# ! $# #0!#/ 0 - 9. 3 $ . !# &#1 + , $ ! ,# /1 #0!#/ 0 " 0!/ / # / !# !+ 2,5 + 3 /! " 3# & + / / / / CuSO4 H2O2 ( ). ) 0,8 + / / / &! !$ #!# " 3 0!# -. - !( ) 9 . & " +!6$ , #- ! !3!#/ - + !$!#1 2. - !$!##1! #0!#/ 0 4! / !## 3 # $ - # 0 0 8/ + . # 3$! ! !3 %/ /1, + 6# %#1 / // , , ) .) !$ #!# " ! +!## 8/ + 73 0! !4! / 3 /%, &/ $ # + + 0! ! - ( +! % !4! / ) .1 !# -, 3 + / / - %3 ## , # 0 / 1 24–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–48 & % +*$ $*8 , "*2 % 4!7 , 7 + +"* "#$ 2& $- ! 3#1! $1 $ &!1** : %:! *+*"7 "*$! $! $! /# 9!- .# # #/!# +!#/1 !# - / / $ #/#1 8 - # 0 0 !# - ( #/ !4! / # $ . !$ #!# ! !3 %/ / 3 &!# - 8**! / 6! /! %# ) $# !# - / / 4! / !## 3 - -/% / # ##!, - /!&!# ! ! - /% # 6$!# 5 # 1 0! !" , /%, &/ ) /! !/ 4! / !## 3 / 3 . # !$ # /%5 !# - # /% $!" / #0!#/ 0 [7]), + 6# !+ " #/!# /!&!# ! ! 1 24–48 & . %#1 * , . - , #$ 0 - $ - #0!#/ 0 - * * +. !3 %/ /1 # 9 3 + . # %#1 1/ 4! / !## 3 / !$ #!# " . # %#1 $ / # 33 + / #1 + / 8** 0 !#/ ) # + ) ( #0!#/ 0 3 /! -+ / $! #0!#/ 0 * * $ !#- #0!#/ 0 - !# -, / /% # !# - . 10). #0!#/ 0 # r $!+ # / +!6$ 8/ + !#/ ) 3 . # $ CL !/ # 5. " $ %#1 $ / !#3 ) + & ! &!#% 6! / " /! %# / / 0,959 $ 0,996, p < 0,041) () 74 ! $ 6!# ! 3). !#- CC /!+ &!/ -+ " 3 + -3 !# - ( 8** 0 - # $! 6 # ! 10. !# 5 ( ) 0,8 + 3 !+ #! +!#!!, , ( &# " !$#!# ! !3 %/ / 54 + !/ - ! 3 #!$ / / &# / &# , &/ +!#/ %#1+ $ 6 /! %# /% ! + ( 38 / !# ! 1+ 8 %#1 !# 5, # 0 H2O2 ( ), +. . # $ !$ #!# " ## + $ . #0!#/ 0 ! ($ ##1! / !/ / 54 !# - 1 / % #$ 0 75 / CL, !#3 !$! !# / CL # # & %#1 8/ . !! $ ## +) # - $! M ± SD). + / C 3 &). ) + / C # $ , ++ / - Cu(CH3COO)2 !$!#1 #!"# - * # 0 - 3 $ ##1+ $ ! ,# /1 !# !+ 2,5 + * * / $ +!#/ #/ ## +, / / 6 !/ 3 + , /! %# + , 6 9 !# - !# #!"# - * # 0 - 6! & / ! #0!#/ 0 " 2,38– 3,57 +, $ /+ , +# !#3 # +( . # 3,57 +,/+ . ! / !$ #!# - $# . # , / 3 +!/# + # 0 # 3 ($ ##1! #! 11. , ++1 . # $ %#1 3 #$ 0 /% # # / !3 $ !# + # "# !#3 &!##1! # + 3 54 - $ !#3 $ - 19! !$ / ! !$! !# , - $! 6 # - /# 9!# - .7!+ , 6 ) # 3 54 + + ! $ 3 / . 30 ! - 3+!#!# 5 /! %# /% ! / / !/ / 54 %#1 $ !3 %/ /1 #- / #0!#/ - #0!#/ 0 - Cu(CH3COO)2+H2O2- $ !# - 3 # !,!#$ # ". 4! / !## 3 ! $ ! 1 6!# / , +# - #0!#/ 0 - !# - $ 24 & .1 /!+ $ . 3 + + / !$ #!# " (, * &! /! %# / #0!#/ 0 - 1+ $ - #/ ## , ( ) !# -. + +. +! 1 . 10) / 6! !# 3 &!# ! # % 3 / $# /! %#1+ 6!# 5 !3 %/ / . # 19!, &!+ . %9! .1 ) ## " 3+!#!# ! 6! /! %# / ! $!#/ * #! / $-/ -). $ / &# " $ ! 0 !$! !# - ## , ( ) $ , ++ 3 !/ - . !! #0!#/ 0 !" 3 $ + + , 3-3 - , $ 3 # . 9 . 1/ !-# - +1 $ # / *!# $ /! .1 + !$!# ! / !/ #1 + / # /! %# 5 #* + 0 5 + , %#1 4! / !## , 3 . 11). !$! !## 5 $ !# - - * # 0 1. # %#1 /% 3 + /% #0!#/ 0 .- !$ #!# " / #0!#/ 0 - & /1 - 3 6!##1! 19! 76 .!## / 8/ " 3 + - / !$ # .!## /!" ) + %#1 # 1 + -# - ! +!#$ !/ - #0!#/ 0 !" %3 !$ #!# " # /% $ 2,38 +,/+ ! !#% /1 * * / $ !#3 - . 04 3$ % # *%2 "#0 ,!$*- ' : &$(7 &!% , & , , 4 + "#$ 8 %*% 4 $! 3.2.3. %$ , "*2 % 4 2&* Cu2+-0!,*%*4 8 *$+ ) Cu2+- #$ 0 #/ ## + # 1 + /! !#% 3 $ / / . 0 !# , 3 /!+ — # - # .4! , -, 1. + " #$ 0 / ,/ ) 0 #, , . 12). /! %# +!$ !#- $ / +!$ !## - ( ! 1! ! # / #- #! . $ + / . . *! #1 / $! . ## " (2,38 +,/+ ): . *! . 3 54 4! / !## +!#-!+1! / ## " +! - !$1 $! $ /! %# , #/ ## , !# - * #$ 0 (1), ## , ## , Cu(CH3COO)2+H2O2 (3). #! 3+ 6# !9 * * / $ +!#-!/ - /# !$1 ) # . 0 #$ 0 !# ! # . 0 +!#!# - + Cu(CH3COO)2 (2) !#3 -!/ -. ;/ / * / !$ 6!## " # 1 !# ## + ) !# ! 3+!#!# ! 12. * / $ #1 3 3+ 6# / %3 3 /! !## ! 3 4! / !## !9 /% 1** ## + Cu2+- #$ 0 !# - # , / , + &! 0 3-3 . 3 . + &! , !4! / $ - , # &!#. ) # - 0 #, BES 77 Cu2+- +!#!# ! * * /#1 . *! / !$ +!#!# +1 * * / # - . *! TES . 3 5/ +!$ . - . 3 54 ! !4!- &#1! + ! 1 Cu [13]. HEPES, #! . 3 54 " $ !/ &#1 .# /%5 / + #/!# %#1 .# # ! . ) / / 2 2 &!# - 6!# ! 4). !" # ! -# ! # !# - . *! #1 .1 3-/ !, + ! +!#/ # + /% # ## , , / #$ 0 +!#/1 ( / !- #/ ## , ) 0!/ / + +!$ - MES # $# + 3 / , &/ . # - 2+ / 10 + , ## " $ ,) , /! %# / 1 ( #0!#/ %3 $1. / 1 6!## $! 6!# ! 5). . %9 " !# - 6 &. ) !3# & /! %# /% # & !+ #$ 0 0!/ / , $-4 +!$ ). 78 ## " 3 + ! ) 9% $ #/# , $!" / # . 0 / - CuSO4+H2O2- $ #/#1" 8**! / 0!/ /# , 2 3 +!/!# . 14, ). # - + ## ". 6,0) !$ #!# " . 14, &! / ! # ", -#/ # " !" . *! #1! %#1 +( + %# " %3 !+1 0!/ /#1" . *! #1" 3+ 6# , .7- #-!/ - # (5 + ) # # /% ) [13]: + +! / $ / !# ! 10 + #/!# /, & / / Cu(CH3COO)2+H2O2 &! / +!#/ Cu2+- !# , / $ #/# , $!" / / !/ / 54 !#3 # & !, 9% , # &! $ #/#1" 8**! / # . 5$ !/ - 8/ + # ! MES # # / Cu2+- #$ 0 $! # ## , ) 0!#/ 0 - -# - # 4! / !## (# 21 – 87 %) !# - . *! #1 ! !/ #$ 0 .!# . # 1 9 8 + #0!#/ 0 - — 20 + . !$ !##1! # $ - 2 +. , / # # +!#-!/ - $ - +1 #! # . 5$ /!+! 0 - # / + MES . 13, ) 6! # + .1 , / % !$ #!# " ! # . 0 ) /! %# !, !3 %/ /! 8 +( 1 $ - !$!# " /# # -31 !/ - +!#-!+ %#1 + $! %# " # - ! !# - [92]. 9% #!3# & /! %# ) 6!# , &/ MES . # / ) !$ #0!#/ 0 - 20–100 + , # + $ - 8 # + %# .1 + , ) , &! -# - . *! #1 $ / ## + ) / . - 3+ 6# /% . # # /% Cu2+-3 . 1 3 & #$ 0 %* # # + +!$ , /!+ #! +!#!! . - /! / ! +1 #! # 9 # /% ) !# ! %3 !+1" # 8/ # # + +!$ [13]. ! /% #/!# 3 ! /# , &/ & / MES (2-+ * + #- - 0!/ / !$! 3# & /! %# , # 0 / ( 0!/ / # . # 0 13. -# ! 20 + %#1 !$ #!# " Cu(CH3COO)2 (4), 2,5 + 3 /! - # # + ! / 3 +. . # & " +!6$ , + %#1 6!# #! / !# - +. !## # 6!, &!+ *! #1" / $ #/# ! $!" / $# -# ! #!# ! 24-& # . +1+ / / , , #/ !# - ##1 / / #/ !+!# # 6 ! ( ## , / / / &! !+ &! !3 3 $! 6 # ! /$! %#1 . # 79 / %#1 .1 8/ 4! /- $ , " / + #1, 0 # /#1" . - %#1 !# - ( 0 # /# , . *! # , !. 5/#1! " 3# & + - !+!##1 . 14, , ). 6& . # . CuSO4+H2O2 # + .1 !# - $! 6 # ! 3# - %# + #!3 . *! !#- (M ± SD). 0 # /# , . *! # , / # + 4. ) ! # H2O2 (3), 2,5 + #/ # $! 6 # ! !# -, #$ - 100 % +!#/ 6!# ! 4! / !## + H2O2 (5). #$ 0 0!/ /# , . *! # , / !# - (1) !$ #!# " !$!#1 ) & ! Cu(CH3COO)2+H2O2- #$ 0 6,8) # ( CuSO4 + 0,8 + !$#!# ! $ ##1 4 8 $! 6 # - / / Cu(CH3COO)2 + 0,8 + 0 # /# , . *! # , .# $! / !/ / 54 " + +!#/ /! 3# &!# - + CuSO4 (2), 2,5 + ## , : 2,5 + &!# ! MES–NaOH . *! # , $ / . 14, , ). ) $# !$ #!# " / #! .1 + , / & - # -# ! . *! #1 14. $! 6 # ! . # $ . 0,8 + %#1 ) 4!, $! 6 # ! ! $ !# - #!# ! $ ##1 4 8 . !! 0 / /# , #$ 0 ! ) . *! # , ## , *! / # # ( , 8/ 3 / % . 2+ !# ! # - - #! .1 . # ) !$ #!# " ! / 1 # 0 - / / ) 0 # /#1" . *! , 3 – 0 / /#1" . *! . 10 + ( , + . # 1 /( -# ! # #/!# . *! #1" !# - # # & %#1 8/ #/ !# ! /# ). ! # / 100 % / !/ / 5- # - # -). !$- (M ± SD). +!#/ !# ! . 3 & - / / %#1 . # 1 Cu(CH3COO)2 ( , !4! / 2 + / / / #/! ! #1+ #!3# & /! %# ! / . # # ! + , CuSO4 ( , . 3 54 6,0) # ( H2O2: 1 – 0!/ /#1" . *! , 2 – # + #1" $ / !# !+ 2,5 + #0!#/ 0 - . *! 8/ / . # - %#1 %#1 80 3& ) + & ! CuSO4+H2O2!# -) # . 5$ !$ #!# " ( 3# & +1. 10 + / / / &! $ / / (1 / / / &! # /% ) . 14, ), $# 0 / /#1" . *! - 3# & +1" #, . /!&!# ! % !, 54 " 8*- 3 &!## , !- $ . 2 !! / , , # . 24-+ & /, -( !# - 8**! / 0 / / #! / % / 1 . # %#1 !$ ##1 ## + + $ #!"9!+ ( 24-+ & !# -) #/ # , &!3 # /%5 / ( ! 24-& !# 0 / /#1! . *! #1! #0!#/ 0 " $ / ! # !$ #!# " / $ ,# , . 14, ). Cu(CH3COO)2+H2O2- #$ 0 ) #! , ( # 6 /! %# / $! 6 # ! !# - 1–6 &. $ #/#1" 8**! / 2 + / . 14, ). !# - - , 10 + . - $# $ %- 0 / /# , . *! 0 / /#1" . *! #1" - - # . %9 " #/ $ #/#1" 8**! / . 14, ). ) !$!##1! $ ##1! 31 5/, &/ . *! #1! !$ #!# " (MES, -# ! # 0! # . # 1! ) 0 ## + # 1 # . 0 3 ## " 1 " / 24 &) &! / !$1 +1 + &! # / $ / , # &!- 4! / !## ! ) . / !$ . # - , #! . -54!, # # -( 3 . / %#1 /!&!# ! ! 1 ) + &! - #/!# # /% / # #, / + ) ! $ ##1 # 9 $! /% !$ 54 ! 1 $1: ) - #$ 0 !# - -+ +!/ #- ) 81 Cu2+&!/ + 8/ . #/ $ #/#1 $ /! %# , ($ 8 ! +. # ", + 6# /#1! !3 %/ /1 $ - 0!# / " 0!# !#3 !$ $! !# -; (2) # 6!# ! +!#!# ! # / -4!" , - !# - * * / - !# - #!3# & /! %# , , !+, &/ $ - 0! !" !$!##1 $ - + " #$ - $! !$ 54 ! # ! #!3 . *! !## " ) Cu2+-3 $ / $ / / &# + # 1 - %# • %#1 . / -/! % / : (1) !$ #!# " $ !# - # #1 31 /% + . 3 !/ - /!&!# ! ! 1 24 & + " #$ 0 !$ # ! .#1 +!#!# - 3 $ &! # 9!, # # ! $ 1 # / !/ - / 1/1+. ) . / + # + # !# $ ) !# # 1 $ - . *! # , +!# /! %# 6! # #, . # 3+ 6# / &! / ! , ) + #$ 0 + . 3 +, !$ #!# " /1) !# - #/ ## +, / / $ !/ +!#/ %- ! !+!## " +!#!# ! $ - !#/!- $! !# #! / • $! 6 # - . . / # + . # " %#1 $ / ) $ # / *!# #0!#/ 0 . # %#1 $ / # 1 + /1. 3+!#!# - !# - * * / $ 0 0 0! . !! / ) • + ) / 4! / !##1! 3 #0!#/ 0 #!#/1 9 !# ! %3 !+1 .#1 4! / !## + . # -5/ # + . # ## !#3 !$ #!# " + &! !$ #!# " . 54 ) - %3 # !/# , # #/!# $ /+ /! %# / !$!# - 8 "#1 8 5&!# - . -, 0! ! ! &#1 !+!##1 8/ ! +!#/ 24 –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u2+- #$ 0 !4! / # & %#1" &! /% 8/ / * /, / # 9 3). 5/ - $ #/# , $!" / /! # - ! 0 " ) $ / / &# # 3 , # ( +. , $ &!+ # /% - # &!# !+ $ ) . %#!! 3 . ), 8**! / + 8**! / # +# /! %#1 (p > 0,04). 84 - #0!#/ 0 , #0!#/ 0 $ #/# , $!" / ! $ !# - . 16, , ). ;**! / 3# & + #! / #/ % # /% #/ /! %# / (48 &) ( / / # /% & %, - 8/ .1 # /% #/ . 8/ / # - & - .1- # 15. ;**! / (1,75 ++ %/+ % ( ) ) $ , #$ 0 #!# ! $ ##1 5 8 # /% #/ 4,76 + $ #/# , + , ) ## + Cu(CH3COO)2 ( ) ! +!#/ $!" / (M ± SD). 85 - # $ ! ,# /1 . / Cu(CH3COO)2+H2O2 ( ). !# 5 !$- # 16. ;**! / (35 ++ %/+ % ) , #$ 0 $ ##1 7 8 $ # /% #/ 95,2 + $ #/# , + , ) ## + Cu(CH3COO)2 ( ) ! +!#/ (M ± SD). 86 $!" / - $ ! ,# /1 # . / Cu(CH3COO)2+H2O2 ( ). !# 5 ( ) !$#!# ! /+!/ + / 6!, &/ / &! # # 8**! / !# - (1 3& # . 0 ! # , ( # /% . # /% 1 . $! ) # + $# " / ) !# - !# - $ $ / + * 8/ $ 3 + $!" / ) %3 # 3 / ) #0!#/ 0 !# - # ! ! ! $- # / # / #/ $ #/# , 35 ++ %/+ % $ (95,2 + /!&!# ! ! 1 6 & / $ #/#1" 8**! / ) #$ 0 & %# " #/!# - 3# & + , ) . $!" / -, / # /%5 . # $ #/# , 8**! / ( %#1 - - $ 1, / &! #! !/ . # /- # 0 0 - 3+!#!# - 8**! - " $ - - #0!#/ 0 # % / %) 48 &) #- (24 . 15, ). & ! !4! . !! 1 $ / 5. " $ 87 . &!##1 8/ &!3 % , $ ! 0 ") / Cu(CH3COO)2+H2O2, / ! /% !# - + $ # + # /% $!" / - $ #/# ! $!" / ). 6 5 # ! # $!+ # / # / &# " # 0 0 . - , 0 ,$ #- 5 &! !$% #- $ $ (4,76 + ), 8**! / !# -, # # / # #/ , 1,75 ++ %/+ % #/!# !# -. ) [6, 51], #/ - / $ - $# " * 3!, # ! ) ! 3- /! !/ ## !# ! /1 5/ - $ / # , # 0! ! 0 " %#1! ! 0 $ - # 0 # /%5 &!##1 8/ / # +1 # . 5$ + Cu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u(CH3COO)2+H2O2. !$ 54 + . 3 +. 0 0 . - - #! ) # 0 0 . %9 - 8**! / , / !# 35 ++ %/+ % Cu(CH3COO)2+H2O2). ) . (Cu(CH3COO)2 # ! $!" / #0!#/ 0 ) /! %# / , # "# - #! - - !# -, 8*- *! / # /% #/ # !+ $ .1 ( $ #/# , $!" / /! %# / .# ! $ 6!# , &/ - . !# -. /! !## # . !! . # 3$# !# ! # 6 8/ . # % ) %#1 ! &!- (48 &) # + $ / ) . 15, ). . 5$ !+1" 8**! / + 6# 0!#/ 0 / / 54 8**! / # - 6$ 54 - 3 1/ /% - .7- # /% #0!#/ 0 . $#1 / / &# 6$!# %#1! $# + . #0!#/ 0 - $ $ $# # 3 - # /$ !##1 8/ ), . %9 - & /% + ! 1, / 1! .#1 /% #! / &! ! #1 ( / * %#1! *!# %#1! &!##1 ) 8/ $ #/#1 ) " / * / /! !",—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p > 0,035). # 17. ;**! / 35 ++ %/+ % 0 ## + # /% $ ) #/ $ #/# , + , $ ! ,# /1 Cu(CH3COO)2+H2O2 $!" / / - !# 5 ( ) !$#!# ! $ ##1 ( ). ) 4 8 ! , #$ +!#/ (M ± SD). ) # ! #!8**! / / &! #$ 0 ) +1 ! 0!/ # #1+ #, . 3# & + ! (p < 0,01) + + 4%5 #0!#/ 0 # ) /!+1 Cu(CH3COO)2+H2O2 1,75 ++ %/+ % $ (4,76 + . 2 !! / , , +1 # . 5$ $ #/# ! $!" / 89 ! ! 0!/ # ( . !, # . 17, ). !$!) .1 / / - #0!#/ 0 # ! . # &!+ !, 0 . 3 (24 & / # / +1 # - $# %#1 $ / #, . 54 - # &!##1 8/ / !# -) ( /! %# , # & ) / /%5 3 $#1 # /% ) 6$!# - !$ ) % #/ $ #/#1" 8**! / $# 5 $ # + #!# !+ - # " / $ + / , . $#1 !$! -!/ - $ $# + . !$!# - $ %#!"9 ( %3 !$ - +, 8$(7 %,-0 8, $! $! + .1 $! 6 4 2" $* 3 &!#1 #! % .!## / $ "#1 -# - # #/!# -3!" = ,—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u(CH3COO)2+H2O2 ( ). !$#!# ! (M ± SD). & ! / *!# %#1 -# - # $ . / + ##1 $ #/# , $!" / $ , #$ 0 !+ /! %# / 1 *!# %#1 # /% #/ !# 5 ( ) / &! / ! .!## / ) 18. ;**! / %0 *! ! # , 6!# , &/ #0!#/ 0 %#1 # .# $ $!" / . 3 ( +. # ! 3$. 4.1.1), !/ # . # %#1 91 $ / 0!/ / #! 31 $ / - ) / !# +, %0 *! - --, $# , /!#$!#0 5 , #! 3+!#-- !#% ) +, # . !! 3$# $ #/# 5 *! # #!3# & /! %# " / + -0 /!&!# ! ! 1 3 & !+!##1 8/ / # /%. !/ - /!&!# !+ & ! Cu(CH3COO)2+H2O2- #$ 0 0 *! $ / ! # # 6 /!&!# ! ! 1 6 & !$ #!# - !$ / %0 *! / !# ( 2 !! 1 ) / # !/ - /1 !/ /!+ / 0 - $ #/# - !+ . $#1! $ $ !## 3 -!/ # / !/. 9% $ , & ! %0 *! /! %# / / ! + 0! ) . 8 , / %0 *!- # /% 8 , - /! # , &! / # , $ %#!"9!! 3 -3-+ $ #/#1 # # %#1 $ !# -. 92 $ / 3 ) / - / $ /% / ! ! 0 " + $# $! $ /1 .7- # /% / 6! . # - - ! ! ## , -3-+ , &#1+ . 3 + + 6# !# ! !# -. . 3 54 - !- + ! $! 6 # ! !$! !##1 #!# 14!##1+ -3 , !/ # - 3 # #!# 14!##1+ ! /% - $ #/# , $!" / .#1! $ "#1! , # # , 0!/ / # # & %# + 8/ ! +! # 3+! #/ !$# ! / #/!# +! / , , 1+ , / $ + + , # %- 4! / !## # 6!, &!+ # /% !/ # ## +1+ 3 4 4 - / 4!# - 8/ " , /! %# / $ #/# - 1 – # 0 / 1 ) $ / / &# $ - ! ! ! / / ! ! 0 #-!/ - [154]. ) !$ #!# " + .1 3 ! /#1+ $ ##1+ +!- . 8 , . 18, ). - #/ , %#1 0!/ / !, . !! 1 6!## ! ## + ) %0 - . 18, ). -!/ - / / * /, &/ #$ 0 - 3# & /! %# 5 !/ # . %9!" $ $ #/# ! $!" / #/! ! #1+ . !+!# ( . # !# -, # - - !# - 54!, 8**! / 8 , ## , ) $! 6 # ! . ; , - / !# - $!+ # / 1 6!## /% / + !#% ) ) 4! /!# - + , &/ !$!- " / [139]. ) + /% 8**! / + /$ 8 - 4.2. "*-$* 9*+& :*"#$(7 !$ * 2& + , %*+!$ , $! $! !&' $*"#$(7 , "*2 % 4!"#$ 8 4 + "#$ 8 %*% 4 % ' $$ % * ,"*-$*- * " , $! $! 4.2.1. 2" $* 2" $* !&' $*"#$(7 2& + , , "*2 % 4!7 ) $#1! # 3 + ! 6# 5 % 3 4 /! #!# - + , / - -/% ( 0!/ 0 /! #, 3 &!# ! / !4! / + #1+ # / # ! $ !/ / #$ 0 21 # / / !##1 !$ #!# " — $! 6 4!, 1! !$ - ! / !$ / -!/ 3- # . # %#1 . 19). ) 8/ + 3 &!##1! / $ #/# , $!" / - 3 , # 0 &#1 -), !$ #!# " /- $! 6 4!, $! #/ ## - #0!#/ 0 100 + # # & %# + 8/ ! ) - -5/ . . (1 & 3 5 8**! / # 0 0 - -5/ !# -) # /% #/ !# - ( / !# ! ## ! Cu(CH3COO)2+H2O2). . !! $ # 6 !/ -, /! %#1 /- .# /% $ 6! &! # /%, &/ % .1 / !! ## , !# - #/ / !/ - / / / &! # /%5 /! -!/ + 1 ! , +. # / !/ % ! / $ , - 5, %* # / # / ## , Cu(CH3COO)2+H2O2 ) # $# , / $# / / !# ! $ #/# ! $!" / ) 1 1,— # # 3 +!/# ! #/ #$ 0 ! !$ 6!## " # + (2,3-$ +! 6!# , &/ , / / / &#1 # /% ) / .# ) 3 !6$!# ". !$! !##1" #/! ! !## " * +!), 1 , ) /! %#1 #! #) , 5/ ). ) 8/ + #/!# 8**! /1 $ # ( !## , ( #, 0 /! #, 8 , / !# -. 1 3 & , / , # / -# ! / 1 (, / / , # 3+ / &#1 / / - #1! / $ !# ! $ #/#1! $ #/# ! $!" / 3 3# & + " (p < 0,015). -/% # . !/ !/ $ #/# - 8**! / !, / / . # " / . # - % . !! 1 6!##1+, &!+ %#1 # /% # / / $ / .!## / # 6! ) !$ !/ !# - $3 & ! Cu(CH3COO)2+H2O2- !# -. !$!##1! $ ##1! $!+ # / #/ $ #/# , $!" / #/ $ #/ , -/ *!# %# , , / 5/, &/ $ # + 3 +!/# / !# 93 3+!#!# - & !/ - / / , / - ( +. / # * + 0 "$ 3$. 4.1). * %#1 ! # 3+ $!" / !# !+ SH-, / 3 # +!/ # - $ ! / # 3 $ $!" / #$ 0 # / ## + !#/# / , -3 # ,/ -31 # !+ / 1! - -5/ - [92]. ) %#1" $ !$!# -+, / 3 + $!" / $ 3 / + $ + / , /! !#%5 ! 0 $ . $!/ . - ## .# - ! /% - $ %- . # /% #/ (100 + $#1 " /! !# 8/ / / ! ! 0 " 19. ;**! / * + ) !+ / !/ / 54 " / !$! -!/ -, /! / #1+ , & /# / , , #!"9!! 1 ! ! $# " 4 !/ - / - / ) $ #/# , $!" / + , Cu(CH3COO)2+H2O2 ( ). (M ± SD). 94 -, / / $ ! ,# /1 # ( / !$#!# ! $ ##1 / # !# 5 ( ) 3 8 ! !## ) +!#/ , 8/ + +1 ! .1 / /!# ! + $ #/#1 . 3 54 ! 0 /! - , / / .# /%5, / / / # , / / +!/ « # + %# !» # $ , " / #1, , / / & / ! % ! 0 $# , + # " !- # # . 5$ !/ - 6# / !$ ) , /%, &/ / 0! ! Cu2+ $# " / — , / + # !# +! %5 Cu(CH3COO)2 $ / # - 54!, ) #! / #1" , /# 9!# ! . # /% # / $ / !, / / . %# , + 6!/ .1/% %# , $ / .# /%5 # / ! 1 1 . $ 5/ 1 $!" / 54 -31 !/ & /% .# /%5 + . !- !#/# / [179]. !+ #! +!#!!, +1 # - # 0 0 / . %9 - 8**! / !+!## ) - #1! # !#/ # ## " " / !# - , / / !# # -!/ - $ , / / ! 0 /% ## + /! # !+ $ #1, , / / $ $ #/# ! $!" / !$!# ! , / / -3 # H2O2 %#1! ! ! $# " Cu2+- #$ 0 #! # / # . %9 - . 5$ !+ . !! 1 6!## ! +, $ #/#1 " / #! - -!/ - #! 6 $ ##1+ * / +: 3 ! /# , &/ !# ## # + #/ # + -3 # !# - # / . 3 1 /% . !! +!/ 3 . &!/ # & - +!#%9!" . ( , &#1! # / + ! + !- ! $ SH- . % ' $$ % * ,"*-$*- ! &*$! $! $! 4.2.2. 2" $* !&' $*"#$(7 2& + , , "*2 % 4!7 # (2- + # 8/ # + , + + # * # 0 / # # - # - -!/ - / !$# ! , $1 # # ". $ / .# 6 #, . # & %#1 Cu(CH3COO)2 + H2O2). / 0 #-!/ &#1 / ) - -!/ 6 + /!+, # , &/ / # / ) ! # !&!# 100 + # % # !# ! 3 &!##1 8/ ) 31 !/ #!3# & /! %#1" #/ / # - &#1 3 . ! # /% ) %#1 #$ 0 !$ - / 1" / / / &! . # $ #/#1" 8**! / 95 /!+ . #/!# #/ ## , , / ( $$! 6 ! * #, - -!/ #!. %9 !, # 54!! $!" / / $ - 6# 5 !$ / +!#!# ! #0!#/ 0 %* # 1+ , - %# 5 #! # 5 / !## , ! $#1+ , # 3+! 5& - 0!#/ ! . !! 9 -# ! / + ! (p < 0,008) # 1 - #1+ !4! / + ! 1 3 & + ) 3 %* # ## " #$ 0 3# & $ / ## , #0!### + ) +, # # -( # /%5 /! -!/ #/ . , -3 # #/ !#/#1 +!/ # 20. ;**! / + , # / &#1! + + # #1! ! 1 , # 3+!. / !##1 + !4! / # / ! ) !- ! #/ 1 !$ - / / /) !$ #!# - / -/ , #$ 0 42" % / / , # + # + +!/ # / -# ! + ## " (# / - ## + Cu(CH3COO)2+H2O2. /1, ! $! .#1! . 3 1 /% $ / / &# , , 5/ 4! 1 $ . % #/!# ) , "*2 % 4!7 . # 1! / !##1 # (100 + '&!0 ;5*7 !&' $ ,(7 *%" 6# 5 / , 1 96 / ), - !$ / / - -5/ $! ! . # $! !&!## / !" -#/ # " (# / - 0 / /) .+!#! !4! / %3 5/ - 54 1 ! 0 ") % / (-#/ # - 0 # /, 0 / /). ) 8/ + / ! ! #1! +! / , , #! / 1! 3 8/ #!#/ + ! ( + 3+ 6# , (M ± SD). +!#/ !&' $*"#$(7 2& + / ! - 3 / # #! - !#, # , $ #/# , $!" / !# 5 % ' $$ % * ,"*-$*- 2" $* - / . 3 1 /% # /% #/ $ ! ,# /1 4.2.3. , & ! . !$#!# ! $ ##1 3 8 !/ - $ #/# , $!" / .# /%5 / $ 6 " / . 20). ! # 3+ $! $ #/#1! !" - # - -/ 3 ##1 # / .!##1" #/! ! . 0 # /), ## " ( #0!#/ 0 - 2 10 + -# # !# ! / + . # %#1 0!/ / + +!$ ) #$ 0 !# - (1 3 &) #/ 8/ ) ! 24 & ( . ! #/ 8/ / % # 2,5 + #0!#/ 0 - 21. ;**! / $ ##1 4 8 # !# -) / $ - ) + , ) +!#/ !( !3& !. / / 6! ( %* - !# !+- # 1 / 6! .# 6 3 / & ! // / / , $ #/# ! $!" / & &!##1 $!+ # / , &/ / / / . !! $ #/#1" 8**! /. !/ % #0!#/ 0 /- 2+ . 21, , ). $ #/# , $!" / !# ! Cu(CH3COO)2 ( , / !/ / 54 ! 0 # /, ) / / / # # & %#1 8/ - -!/ . !! 1 6!##1" # /% #/ / CuSO4 ( , 0 # / #$ 0 3# & +1, p < 0,005). - - # # & %#1 / 0 - # H2O2. $ #/# ! $!" / . # 1 + $ #/# ! $!" / ) / / / - / / / &! " ) CuSO4 + H2O2 !+ .1 1 $ / / 2+ (M ± SD). 97 - / 1 !" ! # 0 / / ) ( , + ) 10 + 0,8 + ( , . 3 54 $ . !# !+ H2O2. #0!#- ). !$#!# ! ) #$ 0 ) # " /! !# 1 " - - #/ #/ #0!#/ 0 $!+ # / !" (10 + ). !" /! %# /! . # 1 10 + 9!+ - $ $! 6 # ! !# #! .# . # / " & ! . !! ) - !# - / & - / /+!/ /%, &/ # . !! 1 $ / $ ) /! %# /- (24 &) #/ 6 /! %# , ! 24 & %#1 !$# / /$! %#1 8/ #/! ! # . # % / # + $ 6! + !# - !4! / # . 5$ . !! $ / / /# 3# & +1, p < 0,01). # !# ##1 % 3 &!##1 !, . !! 1 6!## ! $ #/#1" 8**! / ( - / / / &! !$ $ #/# ! $!" / .1" 6!## ! #, . ! Cu(CH3COO)2 + H2O2 %. $ $!" / !+ !# - (3 6 &), $ #/#1! - " / 9% 0 / / - #0!#/ 0 !# - $ / ! # (p < 0,001) # 6 %#1 $ / ) #!# 5 #/ - !+ ( . 21, ! . - , ). ) % 3 54 ! 0 # /, / / / " / [13], + 6# !$! !##1 - #/ $ / / &# &# , + #1 +!$ / , , / !# ! / + 3 /% !# ! #/!# !$! !##1 # 9 8 # - # 54 " . / ! -/ / !/ # -31 # ! + + !&!# 5 Cu2+ !#/ # , !" / /! %# , ! ## " — 3,4, $ - %# /% ! 0 ) / ! # /% Cu2+ (lg + ## " — 5,9 [13]), / 98 1 . 3 54 - . # 1 0! ) " / . !#/ # # # + 9% $ 0 !" , $ - +. ) $ .!!, &!+ +!#!! # / #/1 $ - -#/ # " !, - & / !+ Cu2+ !+!##1 8/ +!+. #! $ + , /!+ +! / !/ / !## , , / !/ / 54 + ! 0 5 ##1! # !# ! ! 0 " ! # !+ / # / !## ! # $ $ #/#1! ! 0 5 . # 1 H2O2 [92]. $ # !/#1 +!#/ +!$ 3 &!##1 ! !+ #! +!#!!, 54!, ! . ! 0 + &! - . 3 ! / 3+! , , $ * $ /1 !4! / + & / !/ -, &/ #1+ $ - & / - , # 0 + Cu2+. # + + -3 # CuII, &/ + 6!/ 3 / $#-/% ! - !# ! 8/ + " / + 6!/ .1/% 3 6!# ! , $ #! ! -/# , &/ $ ! ! # / ) / & / &# $ #/#1 #1 Cu2+ / / - -5/ 1 6!##1! , /%, &/ % + 6!/ , /% 3 ##1 $# 5 * 3 , ,$! - - !$ / # -/ - +!#!! $ / 4! / !## 5 + , / 0 / / !# ! &!# / "& /1 / !&!/ !/ /+ ! - !# 2,9, $ - $!/%, &/ . %9 " #/ - $ #/#1" 8**! / # . !! 1 !$ 6!## " # + ! 3# &!# ! $ , " / ! , /% - $ . $ 5/ 3 # / #/1 #1, # #1 $ %# + $! . .# /%5 8/ .1 1 /+!/ /%, &/ # ! / !## " #/ $!" / - # ! #!# 14!##1+ + / ) $ #/#1 " / #1 8/ ) # & %# !$!## , .! !& /% 8**! / 0! ) / - /! # - 0! ) $ #/#1! 3 $1 *!# %#1 " / . ;/ / * / 3 "- !$ #!# ", !4! / .#1 %# / . 3 1- $ #/ , -!/ 8/ + .# . !$ , /%, &/ !+!# $ !/ $ # ! / + 6!# ! ! 0 " , 0 6 . !! 1 # ! #1+ . 3 + # !$! !##1" / !3 0! ! ) &!##1 8/ . !!). 2 !! 1 6!## - $ #/#1! #/ % !#/# / . - # . 5$ #0!#/ 0 6# . &# , +! # 3+ /, !# - 6 & $ #/ 3 - " - #/$ !#- #0!#/ 0 - 3#-, ! ! / 54!, + !# - 0! # , / - # ! #/ . " / # ! ! -/# , ! 1! .# - $ #/#1! # . 5$ !/ - , #/ $- $ .# / + , $ #/# , $!" / " $ / + , &/ &! !3 - 1- #!# $ #/#1 + &! ! ! $# " $ #/#1 # / ) /% ) . # 1 ! /+ , / &!/ 8/ , , ! %+ " / 3 ## - # ! . !! # 3 " # & %# " #/!# / $ 1- !# # + /! %# /% ($ . &# " # + +!/ / # * + 0 - #/ % ! $ #/#1 & ! 3 / + ) #/ = - -3-+ , / ! 1 . # 1 $&! #!+, &/ # + .1 /! %# / 3 +!54 " # ( +. 19!). ( # ! - .1 . %9 # / ! 0! # #1 .!## - #/ $ #/ + -. /% / $ 0 / /, 1. # 5 [82] 6!# - !# ! / # * + 0 + / / / ! - 3 # $ - 0!/ /- # 3 / "& !$! !## " /! !# 5&!# ! $ ## " , $ #/# ! $!+ # / - !$!# ! $ %#!"9 " - !# - $ #/#1!, +! # 3+ / 6! $ - !9!# - /! #1+ #! /7!+ !+1+ , . !##1 3 !$ 99 " / + #/ # ". - / +, - -!/ $ #/ , / !. !/ - ) !$!##1! &#1 + &! $ ## " , + %# " +!#!# ! 3 #!! &#1 •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• / 3 ) ) %3 # ! - $ #/# , $!" / - .!## /!" # /%5. !+!##1 8/ !# $ - , # 0! &# " #/!# $ #/# , $!" / / ) # 0 0 # /% #/ &#1 5/ $ #/# , $!" / . # - # /% ) $!+ # / !+!##1 8/ &#1 • ;**! / ) .!## / $!" / # /% #/ !$ #/!# 3 . / ## " # + #! 3 ! /#1! # +! 1 !$ #!# " # / ## " # + • ) ! %#1 !$ #!# ", # /% ) !4! / , !+!## , 8/ + %# " 3+ 6# - ) ! , / 6! 0! 0!# " 54 .! ";/ $* )! &#1" !##1 # #, #/ /!+, &/ # - &! , ! / &# + # / $#-!/ # , 0! 3 6! #/ # / 3 ) 3 3 . 2 !! / , , - ) #/ $ #/#1 / , #/ 3# & /! %#1 . / / &! / . " # . - !# ! 0! 0!# /% # . !! 9 - -!/ / " ( - 2 ). &! / !$!##1! # + !$! -!+1 ! ! $#1 +!/ / /% / $ +, + , / ! $#1 , - 2 + # + +!$ . ! / +!/ . # #! $! . !$ / 3 %3 8 .1 /% 9 $! # " /! %# , &! / ! /% #/!# $ / . # / +!#/1 / /# 3 + 6!/ #! #- ) %3 101 ! / %3 / !/ / !## , !3 %/ /1, /# " 0!# ! #!- 3 . %9 , 54 ! ## " # ! # 3 4! / !## 3 2 # ! # * 0 , &/ %#1 $! 6 # - !4! / , ! , $# -/ # * + - %3 . %3 !+1+ +!/ $ + # !$! !# ! 3+ 6- $ #/#1!. -+ +!$ , !# 5 - $ 3 +! !/ #! .- &!##1! +!/ $ + #6! 0 + /! + &! ) - 0!$ 1 +1, / , !# - & ! !$ 6 # 1! - $# , . / / /% # ) 6#1+ +!#/ %#1 $ ##1 !+ , #$ 0 ##1 $ ##1 , +1 * * / $ # /% $!" / .1 " $ $ !3 %/ / . !4! / !, / &! !# - ! ## , +!#/ / 1. . !+1 .1 !+ , 0 - ! " / $# + 3 !9!# " $ ## " ) &#1 8 1/ /% - 1- &! / ! #! !0 * &!!$ #!# " 3 +!/# 3 - $ #/# , $!" / # #,! ! 3 . !- ! # * 0 + &! - !# ! 8 /! %# - / &! " / /- -3 # , 9!# ! !/ - !, $#- + / &! / ! ! . !+ ". ;/ ( / &#!!, # / / * /, &/ 8**! / !$! !##1 ( # ! # - 6# " / / / $ #/#1 & !/ - $ + /% !4! 0 $# . . !+1 - -!/ - # 0 0 %# " + / &!##1 4! / !## %3 , # 3+!), # . 5$ !/ - #!# ! ! / + / / !/ / !## , , , &! #1 $ - /! %#1" / ! $ #/# , . . ! / !$ / , - -!/ - $ / / &# & /# / , ! , ! , # 0 - /! # - . %#1 $ / . . / ) " - $!+ # / & !$ / !## , &/ !$! / # 3 + $!"- & !+1! $ ##1+ +!/ / / # # 1/ - # /% ) - ) / # !- +!/ - #$ 0 ##1+ !# !+ ( 8/ # 0 0 !# - 2 #!0! ! ! / 2 , $# / !$! !# - ) . #0!#/ 0 # 3+!#- 3 % ## " 3 !# 3 #1 ) /# .# $ !, 3 $ / %#1! & ! %#1! $ / - !4! / + / # 1" $ %- !# . # # !+, . # / + # /% ! / #1 !$ - . # , 6!# %3 &#1 , &/ , - 2 , ( / 6 !/ # / %#1 !$ #!# " + / / #0!#/ 0 - ! 1! & 1 $ / , -+ $ . %#1 / 6! / / , +1 ! +!#$ - %#1 0! !$ #!# " $ - !" +!$ 3# & /! %# /!&!# ! ! 1 24 &. ) Cu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—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— *!# #!# 14!##1 # !#!&- %#1 !# - + &! . # 1 . + 6!/ +!6 / &#1 , !# ! # ! &# " $ " # ! - ## , - !# - / !. !/ # ". 3 # / .# /%5 # +! # /!" #!! $ #/#1! / #/!# ## !$ #! 3 ! /# ! . !!). ) - !# ! / 6$!# - # 1 0! !" 1- !# # + # " / + , " / # !# - # ! ! 0 , . !##1 ,* #!- .!## / $!" /- 6 $ #/#1 + , , /%5 3 $ #/#1+ +1 .# #0!#/ 0 " #! 3+!#!## , !# - !$ #!# ", # /% ) 4! / !##1 + (3 & #0!#/ 0 $ / $- . !# - /# 9!# !+ ). +, %#1 #/!# #/ $ ! ) #/ ) # 9!+ +#!# 5, ! -/# /% $! -/% - ) /!&!# ! 24 &, #- . # -# - # 3 3 &!##1 ( # ! /! %# , 1- ) . %9 # / - /! %# , !#3 !# 5 $1 - !$ / . !$ 6!## " # + - ! 1 , " - # + " #$ 0 $! 6 # 5 + &! # $! $ +!$%-3 .!## / # + !#3 - * * / $ ## + !$! - ) +1 3 & 3 ,# (#!3 . *! !## - +!$%- #$ 0 / !4! / - $ 48 & % - " . *! # , $ - 3 &!# - ) ) !# 5 54 " !# !$ 6!## 5 # /% ) ! , # $! $ !# -, &!##1 # + $ ##1 , +1 & / !+, &/ #!3 . *! !## # 1 + / + # ## " !/ # -#/ # " - /. , " 3 6# " .!## /%5 - / / * /, &/ $ - 3 -# - # #/!# &# " + &! " 103 # /% ) $1 .1 # 9!" 3 # 3- &# - 3 + /% 8**! / !# #1 +, . * .1 *!# %# " # # . !! / # %#1 " / # ! !4! / . # %#1 / / 8/ ) ! #1! &!+ !$ #!# - # #1! %#1 - .1 - #/ / +!$%- #$ 0 ## + / # $ . !! + # / 104 # !# ! 3$# # /% 8/ . # & - 8**! / # - /1 / # , %* + % +# , 3 .#1 ! . !! 8**! / -#/ # -) / - # & # - + $ # + /, & !, 19 !- + . # 0 0 !" ) / - # / -4!+ + +!#/ !4! #! 1- #!#. . # 1 .4!+ # ) !$ / $ #/# , 8**! / # #!# ## - 0! $ #/# 5 6!# " + ## -) .1 3 &!##1 /1 ( /# 9!# / ! 1- ( ! 3 - -3!" ( !/ # ##!+ 8/ ! ) !# -. .# #/ $ #/#1!. ) $# - # /% *!- ! &!, " / , #/ /! !#% + %#1 3# &!# " 54!! $!" / ! 1-, & " / . ,/ & ! 8**! / / 6! # . !! 8**! / +!$%5. ## -, !# - ) #1 # $ / #0!#- . #!# 14!##1 $ #/#1! . # 6!#1 + 0!/ / + +!$ # /% $!" / .) ) .# /1 (-#/ # -, #, . #/ .!## / .1 % .4!+ ) % $ %#!"9!+ !# $ #/# , 8**! / /! #1! $ / !# # + / $ #/# , $!" / %#1 / # * + $ #/#1! +! # 3+ #/ # !/- ! 0!/ #) ,$ / , -+ / # /%5 /! - - -/% /! %# / % , $ %#!! / &! #/ / # . !! 8**! / # / $ / 1 # # . # !$ #!# " .1 !# ! 1 , / / / $ & . # , $! 6 4 ! #! /! !## /! /% # / !$! !## , + +!#/ , +), +, &!+ / + 3 &!3 ), .1 %#1 - ## + !# -. # /%5 !# . # !# ! /! !## 9% $ %0 *! , !# - # # , +!$%- #$ 0 %#1! !4! / , 0!/ /, 8 , & - $ / / &# " $ - 8**! / / $!" / 4! / !## $1 ( &!##1 8/ 3 * ) $ $ #/# , 8**! / $ #/#1! + /% +, &!+ / 0 - 8/ $ #/# , . # !# #/ ) %#1! & ! . !! 8**! / / #/ &!+ 8/ 3 # 0 0 , # / &#1 !+!##1 . 3 1 /% . 3 54 ! #1 +- . # 1! / + -0 ) %* / + +!$ . ;**! / ! &!# !+ & - #0!#/ 0 - - . - $ #/# ! $!" / #$ 0 !, # . %9 - #/ ) !# - ( 0!/ / + +!$ !3 - # & ! #! 5 !# ! $!+ # / - -# * # 0 1 # # !0 !# 9 /% !# - . !$ / + &! /% " # 0!# " #/!# 3$!" / 5&!# - # " # ! # & #/ #1 8**! / 8 &! !3 1, 3, 6 # ! $# + 6 5/ - - # + # !+ /#1 !/ #/ - /# !/! %# $ #/#1! 8**! /1, # " 3, -$ . & / /1 + , / /! !$!- #1 !/ &- in vivo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in vitro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u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u- #$ 0 + &! !$ #!# " ) ($ /! %# /% !# -). ) - !# ! # ! #/ !# - $ #/ , #/!# +. # / 2*% *%2 "#0 ,!$$ 8 "* &! % ! / 6. ., 1. &( # . . ! $ , !6$ # // $#. 6. +!$. / - .– 1998.– < 6.– .41–46. #$ !! 2. !" $ 31 # ! 2 // 2 .!# # !$! !# - ! !- # +! /&-# . ., . . )! / /! %# , / ,!#!3! " . ., #/ # . ., > +& .)., " " >. ., . ., 2 .!# . . / #- , # 3+ . ., -9 >. ., !# - 0 - 3 ! / 1 6$!# - // )! !- &#1 3 . ! # ": .. # - ! #: " / #, 1988.– .24–26. – 5 # & -# – $ / ,!# $ $ * . . .. $! .– 1988.– < 11.– .41–43. . ., $ #/ !# ! 9 # . ., ! -# # - #/ &#. / $ # /! /! /-# ;. ., 3. 4. 6!+- . ., 5+ #! 0!#0 // 2 !+ , . ., 0 # +!/ #/ ## " 5+ #! 0!#0 -. & . ., / - + # . ., . $# $ . ., 0! $ ##1+ ! #$ 0 ).– . - %# , ## " !+ - .58.– .: , 1983.– .164–179. 5. 2 . " . . 2 ! : 6. , &! ! $!" / ! / /! %#1 *!# %#1 !$ #!# ".– $ + , 1976.– 260 . 2 .2., !# $ 9 . ., . +!+. # // 2 %/ . . *! ! $# + . +!+. #1.– 1998.– .15.– < 2.– .137– 167. %! 7. / . ., 5. 0 # #!/ $ #/ " !. # .2., 0! # , $ + . ., !# - >. . !" / $ + ! #- // 2 . +!+- ! /#. .– . #1.– 1998.– .15.– < 2.– .177–183. $ + 8. >. . . $#1! $ 1 #/ $ #/1 // 1998.– < 7.– .43–51. # 9. # 10. ! ! ! -# ! . . ., # ! 3 !# ! &#1 * + $ / # 6., ) / $6 !"&! . ., $ + . +!$. // . / + # / + # !, + : &!/ # - / + # + .– 1984.– < 4.– .91–94. + - . + -.– .: , ! /1 / - 1999.– 176 . 11. !! . ., / ,!#!3 // ! /#. >. . . $# .– 1999.– < 2.– .22–26. 108 $ %#1! E **! % . / / / 12. # 13. ., ; / # ., ; / &! / " + 6 # ., .– .: , 1994.– 268 . &# . . + .– .: , // . . 1991.– 544 . . # # 14. . , /! %# - + $ * . . 0 - .! .– 1993.– .113.– 1 .1.– .71–81. $# 15. . ., A , !" .2., !$ . . ! 0 - . $# $ # ! #, . %# , 54!" !# - // 2 / # / . . # . +!+. #1.– 1998.– .15.– < 2.– .184–190. / ,#!! 16. #/ . ., B $ # .)., $ #/ / . 3 / . / + # . . , &! +!+. # // 2 # ! %#1" . +!+. #1.– 1998.– .15.– < 2.– .119–135. 17. = ! // 2 3 . . #/ /! ).– .57.– 18. = + ! 5+ #! 0!#0 -. !# !$!# ! + 3 . + # 3+ . # +! 5+ #! 0!#0 - 6 .: / , , . . !.!## .)., $ / 0 - +!/ . 3+ . ., /! %#1" / ! #$ +!#/ %#1! // #*., /#1 / #!" // 2 !- , 1983.– .3–29. # . ., . . . # &#. : !+ ).– .58.– . # . ., +!/ . 0! , 1982.– .3–36. #/ ## - . . . 0! #1 !, -0 .: + # ). ., 2 19. / ! $!" 2. . $#1! # /-)!/! . ,, 15–17 +!# 1 ! /1 !+!## " /. 1998 ,.– ).., 1998.– .362–364. 20. !. # 0!# . ., 2 .!# #/ /! %# " /! $ 21. !. # >. . . ., ! ! #/ # / + . ., 3+1 $ + >. . +!#!# !+ 6! / &#1 .. $! .– 1988.– < 5.– .59–62. // . ., / # >. ., ! ! # >. ., 2 .!# $ #/# - / . ., # /% 1 / 5. 0 " // ! /#. $ + .2., .– 1999.– < 2.– .15–22. 22. + %&! . ., . . $1, /! $1 /! ! 3.– )..: . . / -# ! ! !- ) /! ) ! , 1995.– 304 . 23. % # , " >. ., !# - # #%! $ . ., ) / #/ ! . ., -.0! $ #/# " 3 4 /1 109 $!/!" 3 !, # 1- + $! 6 # !+ +!/ /1 !+!## " . +! + / # // #$ +!#/ %#1! . # &#. : #*., $#1! # /-)!/! . ,, 15–17 ! - /. 1998 ,.– ).., 1998.– .435–439. !#+ # 24. / +!/ . . !$ #!# ".– .: 3%+!# . ., 25. # 26. 3. !/ $1 ! !! . ., !$! !# - , # &! - +!# *! .)., !, # , # / . #&!# . ., %# /% +!+. # + / . . #$ " in . +!+. #1.– 1994.– .11.– < 2.– .169–173. # # ! " # + -, 1975.– 360 . -# ! α-/ vitro // 2 &! 3! . ., - #/ !3# &! # . ., $ #/# , $!" / ! . 3 &!# . ., .. / . # &# - - @- / # . // / &! " #0!#/ 0 . . . $#1! #*., $ ## - 1 . - + !# , 19–22 !#/. 1999 ,.– + !# , 1999.– .66–67. # 27. # . ., +! 3! . ., 2! !# 3 . ! # , 28. >. . ! $!&# - . $# $ / " .2., 2! ,! % # . . $ /!+1.– +1 %#1! 0! 1 # - ., 2000.– 69 . 3 + $!" / ! !/ + .– .: , 1986.– 240 . 29. "0! . ., #! / 1 # ! / 0 $ $ #/# " /!+1 // #1 # 31. )! # ! ! , , $ 3 &! . # ! %+ # –2000.– . , 30. . ., - 0!# . . # ! #! !0 * &! !# ! ! /$! !# ! $ " , / / " -# #/ $!+ - ! /! / !#- , , $, 2000.– .109–114. + - +!+. #.– 3 + $!" / . . " ! / .: , 1991.– 255 . # , $ // 2 + -.– 1997.– .62.– < 12.– .1571–1578. 32. ) + . ., / + // .. +. 6.– 1986.– < 4.– .35–40. / &! " 33. ) * # , #* 3 , !.!$% / + 1 1 " / . . !+ $ +, $ β- / + # !6$ # // + ;. 2 + - +!+. #.– .: , 1985.– 110 . 110 " . !3# $#. 6. +!$. .26–27. 34. ##1 + #/+ / # #! # 6 !/ & / / +! /# /% / 9!+ &! , . / ! $0 # - / + 6& # .– 1998.– < 4.– /! 35. . ., ) !0 - .)., /! 4!# - / + # , 2 / 3 ! . ., !+1! & . . # + +!$ /! %#1! . # " !- / ". // + -.– 1997.– .62.– < 12.– .1648–1654. # 36. # 1! +!/ . . " . 3 // /! %#1" 6.– 1998.– < 1.– .8–15. 37. 9 # $ . ., # , 38. ; #3 . . !/ $1.– .: . ., $ &# !# - $ . , #! 1 +!+. #1 6 – . ., !! . . #/ % ! !- , &! ! +!+. #1. , 1993.– 182 . /# " !/ // 2 , 1990.– .13–61. 39. ; ! / ##1" &!.# / / / ! / StatSoft, Inc.– : StatSoft, Inc., 1999. URL: http://www.statsoft.ru/home/textbook/default.htm. 40. Ahsan H., Parveen N., Khan N.U., Hadi S.M. Pro-oxidant, anti-oxidant and cleavage activities on DNA of curcumin and its derivatives demethoxycurcumin and bisdemethoxycurcumin // Chem. Biol. Interact.– 1999.– Vol.121.– < 2.– P.161–175. 41. Aruoma O.I. Characterization of drugs as antioxidant prophylactics // Free Radical Biol. Med.– 1996.– Vol.20.– < 5.– P.675–705. 42. Aruoma O.I. Free radicals, oxidative stress, and antioxidants in human health and disease // J. Amer. Oil Chem. Soc.– 1998.– Vol.75.– P.199–212. 43. Ayres S., Tang M., Subbiah M.T. Estradiol-17β as an antioxidant: Some distinct features when compared with common fat-soluble antioxidants // J. Lab. Clin. Med.– 1996.– Vol.128.– < 4.– P.367–375. 44. Baba N., Daido H., Kosugi T., Miyake M., Nakajima S. Analysis of glycerophospholipid hydroperoxides by iron spray mass spectrometry // Biosci. Biotechnol. Biochem.– 1998.– Vol.62.– < 1.– P.160–163. 45. Babiak R.M., Campello A.P., Carnieri E.G., Oliveira M.B. Methotrexate: Pentose cycle and oxidative stress // Cell Biochem. Funct.– 1998.– Vol.16.– < 4.– P.283–293. 46. Batzri S., Korn E.D. Single bilayer liposomes prepared without sonication // Biochim. Biophys. Acta.– 1973.– V.298.– P.1015–1019. 47. Beckman K.B., Ames B.N. Oxidative decay of DNA // J. Biol. Chem.– 1997.– Vol.272.– P.19633–19636. 111 48. Berger T.M., Polidori M.C., Dabbagh A., Evans P.J., Halliwell B., Morrow J.D., Roberts L.J., Frei B. Antioxidant activity of vitamin C in iron-overloaded human plasma // J. Biol. Chem.– 1997.– Vol.272.– P.15656–15660. 49. Berlett B.S., Stadtman E.R. Protein oxidation in aging, disease, and oxidative stress // J. Biol. Chem.– 1997.– Vol.272.– P.20313–20316. 50. Bigwood T., Delve R., Read G. A novel iron (III) catalyzed degradation of aliphatic aldehydes to their lower homologues with implications for lipid peroxidation chemistry // J. Chem. Soc., Chem. Commun.– 1990.– < 10.– P.776–778. 51. Bonnefont-Rousselot D., Segaud C., Jore D., Delattre J., Gardes-Albert M. Antioxidant effect of probucol on RO2•/O2•–-induced peroxidation of human low-density lipoproteins // Radiat. Res.– 1999.– Vol.151.– < 3.– P.343–353. 52. Borders C.L. (Jr.), Fridovich I. A comparison of the effects of cyanide, hydrogen peroxide, and phenylglyoxal on eucaryotic and procaryotic Cu,Zn superoxide dismutases // Arch. Biochem. Biophys.– 1985.– Vol.241.– < 2.– P.472–476. 53. Buettner G.R., Jurkiewicz B.A. Catalytic metals, ascorbate and free radicals: Combinations to avoid // Radiat. Res.– 1996.– Vol.145.– < 5.– P.532–541. 54. Burkitt M.J., Milne L. Hydroxyl radical formation from CuII-trolox mixtures: Insights into the pro-oxidant properties of α-tocopherol // FEBS Lett.– 1996.– Vol.379.– < 1.– P.51–54. 55. Bush A.I. Metals and neuroscience // Curr. Opin. Chem. Biol.– 2000.– Vol.4.– P.184– 191. 56. Cai L., Tsiapalis G., Cherian M.G. Protective role of zinc-metallothionein on DNA damage in vitro by ferric nitrilotriacetate (Fe-NTA) and ferric salts // Chem. Biol. Interact.– 1998.– Vol.115.– < 2.– P.141–151. 57. Cardoso S.M., Pereira C., Oliveira C.R. The protective effect of vitamin E, idebenone and reduced glutathione on free radical mediated injury in rat brain synaptosomes // Biochem. Biophys. Res. Commun.– 1998.– Vol.246.– < 3.– P.703–710. 58. Clare D.A., Duong M.N., Darr D., Archibald F., Fridovich I. Effects of molecular oxygen on detection of superoxide radical with nitroblue tetrazolium and on activity stains for catalase // Anal. Biochem.– 1984.– Vol.140.– < 2.– P.532–537. 112 59. Darr D., Fridovich I. Inhibition of catalase by 3,3'-diaminobenzidine // Biochem. J.– 1985.– Vol.226.– < 3.– P.781–787. 60. Davies K.J. Oxidative stress: The paradox of aerobic life // Biochem. Soc. Symp.– 1995.– Vol.61.– P.1–31. 61. Day B.J., Shawen S., Liochev S.I., Crapo J.D. A metalloporphyrin superoxide dismutase mimetic protects against paraquat-induced endothelial cell injury, in vitro // J. Pharmacol. Exp. Ther.– 1995.– Vol.275.– < 3.– P.1227–1232. 62. Dean R.T., Fu S., Stocker R., Davies M.J. Biochemistry and pathology of radicalmediated protein oxidation // Biochem. J.– 1997.– Vol.324.– P.1–18. 63. Denicola A., Souza J.M., Radi R. Diffusion of peroxinitrite across erythrocyte membranes // Proc. Natl. Acad. Sci. USA.– 1998.– Vol.95.– P.3566–3571. 64. Descampiaux B., Cotelle N., Catteau J.P., Peucelle C., Leroux J.M., Erb F. Cytotoxicity of lindane and paraquat to human hepatoma cell lines // Bull. Environ. Contam. Toxicol.– 1999.– Vol.62.– < 1.– P.16–24. 65. De Zwart L.L., Meerman J.H.N., Commandeur J.M.N., Vermeulen N.P.E. Biomarkers of free radical damage: Applications in experimental animals and in humans // Free Radical Biol. Med.– 1999.– Vol.26.– P.202–226. 66. Draper H.H., Squires E.J., Mahmoodi H., Wu J., Agarwal S., Hadley M. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials // Free Radical Biol. Med.– 1993.– Vol.15.– P.353–363. 67. Dyatlov V.A., Makovetskaia V.V., Leonhardt R., Lawrence D.A., Carpenter D.O. Vitamin E enhances Ca2+-mediated vulnerability of immature cerebellar granule cells to ischemia // Free Radical Biol. Med.– 1998.– Vol.25.– < 7.– P.793–802. 68. Ekström T., Stahl A., Sigvardsson K., Högberg J. Lipid peroxidation in vivo monitored as ethane exhalation and malondialdehyde excretion in urine after oral administration of chloroform // Acta Pharmacol. Toxicol.– 1986.– Vol.58.– P.289–296. 69. Esterbauer H. Estimation of peroxidative damage. A critical review // Pathol. Biol. Paris.– 1996.– Vol.44.– < 1.– P.25–28. 70. Esterbauer H., Cheeseman K.H. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal // Methods Enzymol.– 1990.– Vol.186.– P.407–421. 113 71. Esterbauer H., Lang J., Zadravec S., Slater T.F. Detection of malonaldehyde by highperformance liquid chromatography // Methods Enzymol.– 1984.– Vol.105.– P.319– 328. 72. Esterbauer H., Schaur R.J., Zollner H. Chemistry and biochemistry of 4hydroxynonenal, malonaldehyde and related aldehydes // Free Radical Biol. Med.– 1991.– Vol.11.– P.81–128. 73. Evans P., Lyras L., Halliwell B. Measurement of protein carbonyls in human brain tissue. // Methods Enzymol.– 1999.– Vol.300.– P.145–156. 74. Frei B., Forte T.M., Ames B.N., Cross C.E. Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma // Biochem. J.– 1991.– Vol.277.– P.133–138. 75. Fridovich I. Biological effects of the superoxide radical // Arch. Biochem. Biophys.– 1986.– Vol.247.– < 1.– P.1–11. 76. Fridovich I. Superoxide anion radical (O2–⋅), superoxide dismutases, and related matters // J. Biol. Chem.– 1997.– Vol.272.– P.18515–18517. 77. Fridovich I. Oxygen toxicity: A radical explanation // J. Exp. Biol.– 1998.– Vol.201.– P.1203–1209. 78. Fuchs J. Potentials and limitations of the natural antioxidants RRR-α-tocopherol, Lascorbic acid and β-carotene in cutaneous photoprotection // Free Radical Biol. Med.– 1998.– Vol.25.– < 7.– P.848–873. 79. Fuchs J., Kern H. Modulation of UV-light-induced skin inflammation by D-alphatocopherol and L-ascorbic acid: a clinical study using solar simulated radiation // Free Radical Biol. Med.– 1998.– Vol.25.– < 9.– P.1006–1012. 80. Galey J.B., Destree O., Dumats J., Pichaud P., Marche J., Genard S., Bracciolli G., Le Capitaine L., Plessix H., Brambilla L., Cantoni O. Protection of U937 cells against oxidative injury by a novel series of iron chelators // Free Radical Biol. Med.– 1998.– Vol.25.– < 8.– P.881–890. 81. Gallwitz H., Bonse S., Martinez-Cruz A., Schlichting I., Schumacher K., KrauthSiegel R.L. Ajoene is an inhibitor and subversive substrate of human glutathione reductase and Trypanosoma cruzi trypanothione reductase: Crystallographic, kinetic, and spectroscopic studies // J. Med. Chem.– 1999.– Vol.42.– < 3.– P.364–372. 114 82. Goldstein S., Meyerstein D., Czapski G. The Fenton reagents // Free Radical Biol. Med.– 1993.– Vol.15.– P.435–445. 83. Gordon M.H., Roedig-Penman A. Antioxidant activity of quercetin and myricetin in liposomes // Chem. Phys. Lipids.– 1998.– Vol.97.– < 1.– P.79–85. 84. Groves J.T. Peroxinitrite: Reactive, invasive and enigmatic // Curr. Opin. Chem. Biol.– 1999.– Vol.3.– P.226–235. 85. Guichardant M., Taibi-Tronche P., Fay L.B., Lagarde M. Covalent modifications of aminophospholipids by 4-hydroxynonenal // Free Radical Biol. Med.– 1998.– Vol.25.– < 9.– P.1049–1056. 86. Gutteridge J.M.C. Antioxidant properties of the proteins caeruloplasmin, albumin and transferrin. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritis // Biochim. Biophys. Acta.– 1986.– Vol.869.– P.119–127. 87. Gutteridge J.M.C., Quinlan G.J. Antioxidant protection against organic and inorganic oxygen radicals by normal human plasma: The important primary role for iron-binding and iron-oxidizing proteins // Biochim. Biophys. Acta.– 1992.– Vol. 1159.– P.248– 254. 88. Haber F., Weiss J.J. The catalytic decomposition of H2O2 by iron salts // Proc. R. Soc. London [Biol.].– 1934.– Vol.A147.– P.332–351. 89. Halliwell B. How to characterize a biological antioxidant // Free Radical Res. Commun.– 1990.– Vol.9.– P.1–32. 90. Halliwell B., Aruoma O.J. DNA damage by oxugen-derived species: Its mechanism and measurement in mammalian systems // FEBS Lett.– 1991.– Vol.281.– < 1–2.– P.9–19. 91. Halliwell B., Chirico S. Lipid peroxidation, its mechanism, measurement and significance // Am. J. Clin. Nutr.– 1993.– Vol.57.– P.715S–725S. 92. Halliwell B., Gutteridge J.M.C. Role of free radicals and catalytic metal ions in human disease: An overview // Methods Enzymol.– 1990.– Vol.186.– P.1–85. 93. Halliwell B., Gutteridge J.M.C. The antioxidants of human extracellular fluids // Arch. Biochem. Biophys.– 1990.– Vol.280.– P.1–8. 94. Halliwell B., Gutteridge J.M.C. The definition and measurement of antioxidants in biological systems // Free Radical Biol. Med.– 1995.– Vol.18.– < 1.– P.125–126. 115 95. Halliwell B., Gutteridge J.M.C. Free Radicals in Biology and Medicine.– Oxford: Oxford University Press, 1999. 96. Halliwell B., Hu M.L., Louie S., Duvall T.R., Tarkington B.R., Motchnik P., Cross C.E. Interaction of nitrogen dioxide with human plasma. Antioxidant depletion and oxidative damage // FEBS Lett.– 1992.– Vol.313.– P.62–66. 97. Halpner A.D., Handelman G.J., Harris J.M., Belmont C.A., Blumberg J.B. Protection by vitamin C of loss of vitamin E in cultured rat hepatocytes // Arch. Biochem. Biophys.– 1998.– Vol.359.– < 2.– P.305–309 98. Henle E.S., Linn S. Formation, prevention, and repair of DNA damage by iron/hydrogen peroxide // J. Biol. Chem.– 1997.– Vol.272.– P.19095–19098. 99. Hermes-Lima M., Nagy E., Ponka P., Schulman H.M. The iron chelator pyridoxal isonicotinoyl hydrazone (PIH) protects plasmid pUC-18 DNA against •OH-mediated strand breaks // Free Radical Biol. Med.– 1998.– Vol.25.– < 8.– P.875–880. 100. Hu M.L., Louie S., Cross C.E., Motchnik P., Halliwell B. Antioxidant protection against hypochlorous acid in human plasma // J. Lab. Clin. Med.– 1992.– Vol.121.– P.257–262. 101. Impact of deprenyl and tocopherol treatment on Parkinson's disease in DATATOP patients requiring levodopa. Parkinson Study Group // Ann. Neurol.– 1996.– Vol.39.– < 1.– P.37–45. 102. Inanami O., Watanabe Y., Syuto B., Nakano M., Tsuji M., Kuwabara M. Oral administration of (-)catechin protects against ischemia-reperfusion-induced neuronal death in the gerbil // Free Radical Res.– 1998.– Vol.29.– < 4.– P.359–365. 103. Ischiropoulos H., al-Mehdi A.B. Peroxinitrite-mediated oxidative protein modifications // FEBS Lett.– 1995.– Vol.364.– P.279–282. 104. Jiang J., Xu Y., Klaunig J.E. Induction of oxidative stress in rat brain by acrylonitrile (ACN) // Toxicol. Sci.– 1998.– Vol.46.– < 2.– P.333–341. 105. Kagan V., Serbinova E., Packer L. Antioxidant effects of ubiquinones in microsomes and mitochondria are mediated by tocopherol recycling // Biochem. Biophys. Res. Commun.– 1990.– Vol.169.– < 3.– P.851–857. 116 106. Kakkar P., Mehrotra S., Viswanathan P.N. Influence of antioxidants on the peroxidative swelling of mitochrondria in vitro // Cell. Biol. Toxicol.– 1998.– Vol.14.– < 5.– P.313–321. 107. Kawabata T., Schepkin V., Haramaki N., Phadke R.S., Packer L. Iron coordination by catechol derivative antioxidants // Biochem. Pharmacol.– 1996.– Vol.51.–< 11.– P.1569–1577. 108. Kissner R., Nauser T., Bugnon P., Lye P.G., Koppenol W.H. Formation and properties of peroxinitrite as studied by laser flash photolysis, high-pressure stopped-flow technique, and pulse radiolysis // Chem. Res. Toxicol.– 1998.– Vol.10.– P.1285–1292. 109. Klein R.A. The detections of oxidation in liposome preparations // Biochim. biophys. acta.– 1970.– Vol.210.– < 3.– P.486–489. 110. Kojima S., Ona S., Iizuka I., Arai T., Mori H., Kubota K. Antioxidative activity of 5,6,7,8-tetrahydrobiopterin and its inhibitory effect on paraquat-induced cell toxicity in cultured rat hepatocytes // Free Radical Res.– 1995.– Vol.23.– < 5.– P.419–430. 111. Kontush A., Meyer S., Finckh B., Kohlschutter A., Beisiegel U. α-Tocopherol as a reductant for Cu(II) in human lipoproteins. Triggering role in the initiation of lipoprotein oxidation // J. Biol. Chem.– 1996.– Vol.271.– < 19.– P.11106–11112. 112. Korytowski W., Geiger P.G., Girotti A.W. Lipid hydroperoxide analysis by highperformance liquid chromatography with mercury cathode electrochemical detection // Methods Enzymol.– 1999.– Vol.300.– P.23–33. 113. Kuhlmann M.K., Burkhardt G., Horsch E., Wagner M., Kohler H. Inhibition of oxidant-induced lipid peroxidation in cultured renal tubular epithelial cells (LLC-PK1) by quercetin // Free Radical Res.– 1998.– Vol.29.– < 5. P.451–460. 114. Lacy F., Gough D.A., Schmid-Schonbein G.W. Role of xanthine oxidase in hydrogen peroxide production // Free Radical Biol. Med.– 1998.– Vol.25.– < 6.– P.720–727. 115. Lawrence G.D., Cohen G. Concentrating ethane from breath to monitor lipid peroxidation in vivo // Methods Enzymol.– 1984.– Vol. 105.– P.305–311. 116. Lawson J.A., Rokach J., FitzGerald G.A. Isoprostanes: Formation< analysis and use as indices of lipid peroxidation in vivo // J. Biol. Chem.– 1999.– Vol.274.– < 35.– P.24441–24444. 117 117. Lee A.Y., Chung S.S. Contributions of polyol pathway to oxidative stress in diabetic cataract // FASEB J.– 1999.– Vol.13.– < 1.– P.23–30. 118. Liebler D.C., McClure T.D. Antioxidant reactions of β-carotene: Identification of carotenoid-radical adducts // Chem. Res. Toxicol.– 1996.– Vol.9.– < 1.– P.8–11. 119. Liochev S.I., Fridovich I. Lucigenin as mediator of superoxide production: Revisited // Free Radical Biol. Med.– 1998.– Vol.25.– < 8.– P.926–928. 120. Mao S.J.T., Yates M.T., Jackson R.L. Antioxidant activity and serum levels of probucol and probucol metabolites // Methods Enzymol.– 1994.– Vol.234.– P.505–513. 121. Marla S.S., Lee J. Groves J.T. Peroxinitrite rapidly permeates phospholipid membranes // Proc. Natl. Acad. Sci. USA.– 1997.– Vol.94.– P.14243–14248. 122. Martin C., Barturen K., Martinez R., Lacort M., Ruiz-Larrea M.B. In vitro inhibition by estrogens of the oxidative modifications of human lipoproteins // J. Physiol. Biochem.– 1998.– Vol.54.– < 4.– P.195–202. 123. Mates J.M., Sanchez-Jimenez F. Antioxidant enzymes and their implications in pathophysiologic processes // Front. Biosci.– 1999.– Vol.4.– P.D339–D345. 124. Matsuo T., Shinzawa H., Togashi H., Aoki M., Sugahara K., Saito K., Saito T., Takahashi T., Yamaguchi I., Aoyama M., Kamada H. Highly sensitive hepatitis B surface antigen detection by measuring stable nitroxide radical formation with ESR spectroscopy // Free Radical Biol. Med.– 1998.– Vol.25.– < 8.– P.929–935. 125. May J.M., Qu Z., Morrow J.D. Interaction of ascorbate and α-tocopherol in resealed human erythrocyte ghosts. Electron transfer and protection from lipid peroxidation // J. Biol. Chem.– 1996.– Vol.271.– < 18.– P.10577–10582. 126. May J.M., Mendiratta S., Hill K.E., Burk R.F. Reduction of dehydroascorbate to ascorbate by the selenoenzyme thioredoxin reductase // J. Biol. Chem.– 1997.– Vol.272.– P.22607–22610. 127. Mayer B., Pfeiffer S., Leopold E., Muller J., Weser U., Schmidt K. Structural and functional analogs of CuZn superoxide dismutase inhibit rat brain nitric oxide synthase by interference with the reductase (diaphorase) domain // Neurosci. Lett.– 1996.– Vol.209.– < 3.– P.169–172. 118 128. Melchiorri D., Reiter R.J., Sewerynek E., Hara M., Chen L., Nistico G. Paraquat toxicity and oxidative damage. Reduction by melatonin // Biochem. Pharmacol.– 1996.– Vol.51.– < 8.– P.1095–1099. 129. Miller E.K., Fridovich I. A demonstration that O2– is a crucial intermediate in the high quantum yield luminescence of luminol // Free Radical Biol. Med.– 1986.– Vol.2.– < 2.– P.107–110. 130. Morel Y., Barouki R. Repression of gene expression by oxidative stress // Biochem. J.– 1999.– Vol.342.– P.481–496. 131. Müller A., Sies H. Assay of ethane and pentane from isolated organs and cells // Methods Enzymol.– 1984.– Vol.105.– P.311–319. 132. Multhaup G., Ruppert T., Schlicksupp A., Hesse L., Beher D., Masters C.L., Beyereuther K. Reactive oxygen species and Alzheimer’s disease // Biochem. Pharmacol.– 1997.– Vol.54.– P.533–539. 133. Nagano T., Fridovich I. Superoxide radical from xanthine oxidase acting upon lumazine // J. Free Radical Biol. Med.– 1985.– Vol.1.– < 1.– P.39–42. 134. Naguib Y.M.A. A fluorometric method for measurement of peroxyl radical scavenging activities of lipophilic antioxidants // Anal. Biochem.– 1998.– Vol.265.– < 2.– P.290– 298. 135. Nardini M., Pisu P., Gentili V., Natella F., Di Felice M., Piccolella E., Scaccini C. Effect of caffeic acid on tert-butyl hydroperoxide-induced oxidative stress in U937 // Free Radical Biol. Med.– 1998.– Vol.25.– < 9.– P.1098–1105. 136. O'Donovan D.J., Katkin J.P., Tamura T., Husser R., Xu X., Smith C.V., Welty S.E. Gene transfer of mitochondrially targeted glutathione reductase protects H441 cells from t-butyl hydroperoxide-induced oxidant stresses // Amer. J. Respir. Cell. Mol. Biol.– 1999.– Vol.20.– < 2.– P.256–263. 137. Offer T., Mohsen M., Samuni A. An SOD-mimicry mechanism underlies the role of nitroxides in protecting papain from oxidative inactivation // Free Radical Biol. Med.– 1998.– Vol.25.– < 7.– P.832–838. 138. Ohshima H., Yoshie Y., Auriol S., Gilibert I. Antioxidant and pro-oxidant actions of flavonoids: Effects on DNA damage induced by nitric oxide, peroxynitrite and nitroxyl anion // Free Radical Biol. Med.– 1998.– Vol.25.– < 9.– P.1057–1065. 119 139. Olson J.A. Benefits and liabilities of vitamin A and carotenoids // J. Nutr.– 1996.– Vol.126.– < 4, supplement.– P.1208S–1212S. 140. Papas A.M. Oil-soluble antioxidants in foods // Lipid-Soluble Antioxidants: Biochemistry and Clinical Application. Ong A.S.H., Packer L., editors.– Basel: Birkhauser Verlag, 1992.– P.123–149. 141. Patel M., Day B.J. Metalloporphyrin class of theurapeutic catalytic antioxidants // Trends Pharmacol. Sci.– 1999.– Vol.20.– P.359–364. 142. Pedersen W.A., Cashman N.R., Mattson M.P. The lipid peroxidation product 4hydroxynonenal impairs glutamate and glucose transport and choline acetyltransferase activity in NSC-19 motor neuron cells // Exp. Neurol.– 1999.– Vol.155.– < 1.– P.1– 10. 143. Picker S.D., Fridovich I. On the mechanism of production of superoxide radical by reaction mixtures containing NADH, phenazine methosulfate, and nitroblue tetrazolium // Arch. Biochem. Biophys.– 1984.– Vol.228.– < 1.– P.155–158. 144. Pippenger C.E., Browne R.W., Armstrong D. Regulatory antioxidant enzymes // Methods Mol. Biol.– 1998.– Vol.108.– P.299–313. 145. Plumb G.W., De Pascual-Teresa S., SantosBuelga C., Cheynier V., Williamson G. Antioxidant properties of catechins and proanthocyanidins: Effect of polymerisation, galloylation and glycosylation // Free Radical Res.– 1998.– Vol.29.– < 4.– P.351–358. 146. Pompella A., Comporti M. Imaging of oxidative stress at subcellular level by confocal laser scanning microscopy after fluorescent derivatization of cellular carbonyls // Amer. J. Pathol.– 1993.– Vol.142.– < 5.– P.1353–1357. 147. Porter N. Chemistry of lipid peroxidation // Methods Enzymol.– 1984. – Vol.105.– P.273–282. 148. Pryor W.A., Castle L. Chemical methods for the detection of lipid hydroperoxides // Methods Enzymol.– 1984.– Vol. 105.– P.293–299. 149. Rae T.D., Schmidt P.J., Pufahl R.A., Culotta V.C., O’Halloran T.V. Undetectable intracellular free copper: The requirement of a copper chaperone for superoxide dismutase // Science.– 1999.– Vol.284.– P.805–808. 150. Ramakrishnan N., Kalinich J.F., McClain D.E. Ebselen inhibition of apoptosis by reduction of peroxides // Biochem. Pharmacol.– 1996.– Vol.51.– < 11.– P.1443–1451. 120 151. Rapola J.M., Virtamo J., Haukka J.K., Heinonen O.P., Albanes D., Taylor P.R., Huttunen J.K. Effect of vitamin E and β-carotene on the incidence of angina pectoris. A randomized, double-blind, controlled trial // JAMA.– 1996.– Vol.275.– < 9.– P.693– 698. 152. Reinheckel T., Noack H., Lorenz S., Wiswedel I., Augustin W. Comparison of protein oxidation and aldehyde formation during oxidative stress in isolated mitochondria // Free Radical Res.– 1998.– Vol.29.– < 4.– P.297–305. 153. Reznick A.Z., Cross C.E., Hu M., Suzuki Y.J., Khwaja S., Safadi A., Motchnik P.A., Packer L., Halliwell B. Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation // Biochem. J.– 1992.– Vol.286.– P.607–611. 154. Rice-Evans C.A., Diplock A.T. Current status of antioxidant therapy // Free Radical Biol. Med.– 1993.– Vol.15.– P.77–96. 155. Rohn T.T., Hinds T.R., Vincenzi F.F. Inhibition of Ca2+-pump ATPase and the Na+/K+-pump ATPase by iron-generated free radicals. Protection by 6,7-dimethyl-2,4di-1-pyrrolidinyl-7H-pyrrolo[2,3-d] pyrimidine sulfate (U-89843D), a potent, novel, antioxidant/free radical scavenger // Biochem. Pharmacol.– 1996.– Vol.51.– < 4.– P.471–476. 156. Rojstaczer N., Triggle D.J. Structure-function relationships of calcium antagonists. Effect on oxidative modification of low density lipoprotein // Biochem. Pharmacol.– 1996.– Vol.51.– < 2.– P.141–150. 157. Saija A., Scalese M., Lanza M., Marzullo D., Bonina F., Castelli F. Flavonoids as antioxidant agents: Importance of their interaction with biomembranes // Free Radical Biol. Med.– 1995.– Vol.19.– P.481–486. 158. Sakagami H., Satoh K., Ida Y., Hosaka M., Arakawa H., Maeda M. Interaction between sodium ascorbate and dopamine // Free Radical Biol. Med.– 1998.– Vol.25.– < 9.– P.1013–1020. 159. Saramet A., Danila G., Paduraru I., Petrariu D., Olinescu R. Direct detection of the antioxidant activity of a new flavonic derivative using the chemiluminescence method // Arzneimittelforschung.– 1996.– Bd 46.– < 5.– S.501–504. 160. Satoh K., Sakagami H. Effect of cysteine, N-acetyl-L-cysteine and glutathione on cytotoxic activity of antioxidants // Anticancer Res.– 1997.– Vol.17.– P.2175–2180. 121 161. Sayre L.M., Perry G., Smith M.A. Redox metals and neurodegenerative disease // Curr. Opin. Chem. Biol.– 1999.– Vol.3.– P.220–225. 162. Scandalios J.G. Oxidative Stress and the Molecular Biology of Antioxidant Defenses.– Plainview: Cold Spring Harbor Laboratory, 1997. 163. Schlotte V., Sevanian A., Hochstein P., Weithmann K.U. Effect of uric acid and chemical analogues on oxidation of human low density lipoprotein in vitro // Free Radical Biol. Med.– 1998.– Vol.25.– < 7.– P.839–847. 164. Selim S. Separation and quantitative determination of traces of carbonyl compounds as their 2,4-dinitrophenylhydrazones by high-pressure liquid chromatography // J. Chromatogr.– 1977.– Vol.136.– P.271–277. 165. Shara M.A., Dickson P.H., Bagchi D., Stohs S.J. Excretion of formaldehyde, malondialdehyde, acetaldehyde and acetone in the urine of rats in response to 2,3,7,8tetrachlorodibenzo-p-dioxin, paraquat, endrin and carbon tetrachloride // J. Chromatogr. B.– 1992.– Vol.576.– P.221–233. 166. Sharman W.M., Allen C.M., van Lier J.E. Photodynamic therapeutics: Basic principles and clinical applications // Drug Discovery Today.– 1999.– Vol.4.– < 11.– P.507– 517. 167. Slater T.F. Overview of methods used for detecting lipid peroxidation // Methods Enzymol.– 1984.– Vol.105.– P.283–293. 168. Souchard J.P., Barbacanne M.A., Margeat E., Maret A., Nepveu F., Arnal J.F. Electron spin resonance detection of extracellular superoxide anion released by cultured endothelial cells // Free Radical Res.– 1998.– Vol.29.– < 5.– P.441–449. 169. Squadrito G.L., Pryor W.A. Oxidative chemistry of nitric oxide: The roles of superoxide, peroxinitrite and carbon dioxide // Free Radical Biol. Med.– 1998.– Vol.25.– P.392–403. 170. Stadtman E.R., Berlett B.S. Reactive oxigen-mediated protein oxidation in aging and disease // Chem. Res. Toxicol.– 1997.– Vol.10.– P.485–494. 171. Steinberg D. Low density lipoprotein oxidation and its pathological significance // J. Biol. Chem.– 1997.– Vol.272.– P.20963–20966. 172. Tadolini B., Franconi F. Carvedilol inhibition of lipid peroxidation. A new antioxidative mechanism // Free Radical Res.– 1998.– Vol.29.– < 5.– P.377–387. 122 173. Tammeveski K., Tenno T.T., Mashirin A.A., Hillhouse E.W., Manning P., McNeil C.J. Superoxide electrode based on covalently immobilized cytochrome c: modelling studies // Free Radical Biol. Med.– 1998.– Vol.25.– < 8.– P.973–978. 174. Tang M., Abplanalp W., Ayres S., Subbiah M.T. Superior and distinct antioxidant effects of selected estrogen metabolites on lipid peroxidation // Metabolism.– 1996.– Vol.45.– < 4.– P.411–414. 175. Tesoriere L., Bongiorno A., Pintaudi A.M., D'Anna R., D'Arpa D., Livrea M.A. Synergistic interactions between vitamin A and vitamin E against lipid peroxidation in phosphatidylcholine liposomes // Arch. Biochem. Biophys.– 1996.– Vol.326.– < 1.– P.57– 63. 176. Thielemann L.E., Bosco C., Rodrigo R., Orellana M., Videla L.A. Effects of bromoethylamine on antioxidant capacity, lipid peroxidation, and morphological characteristics of rat liver // J. Biochem. Mol. Toxicol.– 1999.– Vol.13.– < 1.– P.47–52. 177. Thomas P.D., Poznansky M.J. A modified tetramethylbenzidine method for measuring lipid hydroperoxides // Anal. Biochem.– 1990.– Vol.188.– < 1.– P.226–232. 178. Thomas M.J., Robison T.W., Samuel M., Forman H.J. Detecting and identifying volatile aldehydes as dinitrophenylhydrazones using gas chromatography mass spectrometry // Free Radical Biol. Med.– 1995.– Vol.18.– P.553–557. 179. Ueda J., Saito N., Ozawa T. Detection of free radicals produced from reactions of lipid hydroperoxide model compounds with CuII complexes by ESR spectroscopy // Arch. Biochem. Biophys.– 1996.– Vol.325.– < 1.– P.65–76. 180. Valentine J.S., Wertz D.L., Lyons T.J., Liou L.-L., Goto J.J., Gralla E.B. The dark side of dioxygen biochemistry // Curr. Opin. Chem. Biol.– 1998.– Vol.2.– P.253–262. 181. Walke M., Beckert D., Lasch J. Interaction of UV light-induced alpha-tocopherol radicals with lipids detected by an electron spin resonance prooxidation effect // Photochem. Photobiol.– 1998.– Vol.68.– < 4.– P.502–510. 182. Wallig M.A. Xenobiotic metabolism, oxidant stress and chronic pancreatitis. Focus on glutathione // Digestion.– 1998.– Vol.59.– Suppl.4.– P.13–24. 183. Wardman P., Candeias L.P. Fenton chemistry: An introduction // Radiat. Res.– 1996.– Vol.145.– P.523–531. 123 184. Wede I., Altindag Z.Z., Widner B., Wachter H., Fuchs D. Inhibition of xanthine oxidase by pterins // Free Radical Res.– 1998.– Vol.29.– < 4.– P.331–338. 185. Wiseman H., Cannon M., Arnstein H.R.V. Tamoxifen inhibits RNA and protein synthesis simultaneously in Saccharomyces cerevisiae: Partial protection by antioxidants // Biochem. Soc. Trans.– 1990.– Vol.18.– < 5.– P.877–878. 186. Witting P., Pettersson K., Ostlund-Lindqvist A.M., Westerlund C., Wagberg M., Stocker R. Dissociation of atherogenesis from aortic accumulation of lipid hydro(pero)xides in Watanabe heritable hyperlipidemic rabbits // J. Clin. Invest.– 1999.– Vol.104.– < 2.– P.213–220. 187. Wu T.W., Fung K.P., Wu J., Yang C.C., Weisel R.D. Antioxidation of human low density lipoprotein by unconjugated and conjugated bilirubins // Biochem. Pharmacol.– 1996.– Vol.51.– < 6.– P.859–862. 188. Yagi K., Ishida N., Komura S., Ohishi N., Kusai M., Kohno M. Generation of hydroxyl radical from linolenic acid hydroperoxide in the presence of epinephrine and iron // Biochem. Biophys. Res. Commun.– 1992.– Vol.183.– < 3.– P.945–951. 189. Yagi K., Komura S., Ishida N., Nagata N., Kohno M., Ohishi N. Generation of hydroxyl radical from lipid hydroperoxides contained in oxidatively modified low-density lipoprotein // Biochem. Biophys. Res. Commun.– 1993.– Vol.190.– < 2.– P.386–390. 190. Yamamoto Y., Niki E. Presence of cholesteryl ester hydroperoxide in human blood plasma // Biochem. Biophys. Res. Commun.– 1989.– Vol.165.– < 3.– P.988–993. 191. Yamamoto Y., Niki E., Kamiya Y., Shimasaki H. Oxidation of lipids. 7. Oxidation of phosphatidylcholines in homogeneous solution and in water dispersion. // Biochim. Biophys. Acta.– 1984.– Vol.795.– P.332–340. 192. Yan L.J., Traber M.G., Kobuchi H., Matsugo S., Tritschler H.J., Packer L. Efficacy of hypochlorous acid scavengers in the prevention of protein carbonyl formation // Arch. Biochem. Biophys.– 1996.– Vol.327.– < 2.– P.330–334. 193. Yang W.L., Sun A.Y. Paraquat-induced cell death in PC12 cells // Neurochem. Res.– 1998.– Vol.23.– < 11.– P.1387–1394. 194. Yim M.B., Kang J.H., Yim H.S., Kwak H.S., Chock P.B., Stadtman E.R. A gain-offunction of an amyotrophic lateral sclerosis-associated Cu,Zn-superoxide dismutase 124 mutant: An enhancement of free radical formation due to a decrease in Km for hydrogen peroxide // Proc. Natl. Acad. Sci. USA.– 1996.– Vol.93.– < 12.– P.5709–5714. 195. Zaugg W.S. Spectroscopic characteristics and some chemical properties of Nmethylphenazinium methyl sulfate (phenazine methosulfate) and pyocyanine at the semiquinoid oxidation level // J. Biol. Chem.– 1964.– Vol.239.– < 11.– P.3964–3970. 125 &*" 3 $* 1. & ,$* % ! *% */ % %& +$*7 , "*/*$ 8 0$!/*4 % * "*/*8 ,(' & /$(7 $1 $ &!1*8 !&' $*"#$(7 % +*$ $*8, '&!0 ;5*7%- 2&* "*2 % 4 , 0!,*%*4 % * %2 % '! *$*1*!1** * +"* "#$ % * 2 &* +! *%" $*/ ! # /% # !+ $ ! & #1 $ 3 ## , 1. / . & " +!6$ # !$# + 3 ANOVA. $ / ! # #! / ! -, /!31. !$# + ! 3# &!# [12], & # + $ 1. +# /! %#1+ ( #!3# & +1+ ( / & # !$!#1 / . , 0 /- ! +!#$ 3 3 & - +!6$ & - +!6$ !$# + ), .1&#1+ 9 #-/1! / . , $ / ! #1+ ( 1$! !#1 0,01 < p < 0,05 0 &#1! !- / / !/ / #1+ ) ( 1$! !#1 #1! . 3# &!# -, A– !$# ! 1. p, ! p < 0,01 +1 / / / &! 2 " 3*&$(4 =&*: 4); %3 - & +1. !/ / !/ ! -/# / $ / ! # / # ! " , 3 6!##1+ & /1 /!3 : +!/ 0 !$!#1 & # + &!/#1! 3# &!# - 0 -+ , 0 ! / .& / - p > 0,05 — */ +). ) 6!# - 1. !# ! HP – #$ 0 - ) $ . !# !+ H2O2 (0,8 + ) CS – #$ 0 - ) $ . !# !+ CuSO4 (2,5 + ) CSHP – #$ 0 - ) CA – #$ 0 - ) CAHP – #$ 0 - ) $ . $ . $ . !# !+ CuSO4 (2,5 + ) H2O2 (0,8 + ) !# !+ Cu(CH3COO)2 (2,5 + ) !# !+ Cu(CH3COO)2 (2,5 + ) 126 H2O2 (0,8 + ) # / / / &! #0!#/ 0 " . # " 3# & + / %#1 & " 1. !$ #!# " 3 A / + / &#1 !#3 / . !$# . 0 ).1.1. ! & # + &! !3 1 & # 0 0 !# - ) A HP CS CSHP CA CAHP X 0,008133 0,703135 0,002401 0,004399 1,26⋅⋅10–10 X 0,016142 0,992044 0,681515 0,010111 X 0,016197 0,034127 0,000048 X 0,628080 0,000974 X 0,000060 HP CS CSHP CA X CAHP # / / / &! #0!#/ 0 " " 3# & + / . # %#1 !# - 3 A HP CS / & " 1. !$ #!# " + / / &#1 !$# !#3 . # 0 0 . 0 ).1.2. ! & # + &! !3 3 & ) A HP CS CSHP CA CAHP X 0,009477 0,114629 0,000111 1,03⋅⋅10–9 7,00⋅⋅10–19 X 0,177560 0,384575 0,047798 0,000003 X 0,041005 0,000257 1,20⋅⋅10–9 X 0,389127 4,24⋅⋅10–7 X 7,06⋅⋅10–11 CSHP CA X CAHP 127 # / / / &! #0!#/ 0 " " 3# & + / . # %#1 !# - 3 A / & " 1. !$ #!# " + / / &#1 !#3 . !$# . 0 ).1.3. ! & # + &! !3 6 & # 0 0 ) A HP CS CSHP CA CAHP X 0,000300 0,005674 0,000162 8,51⋅⋅10–15 2,04⋅⋅10–20 X 0,419408 0,556853 0,001111 1,34⋅⋅10–7 X 0,175412 5,85⋅⋅10–6 1,72⋅⋅10–10 X 0,010195 2,50⋅⋅10–7 X 3,25⋅⋅10–8 HP CS CSHP CA X CAHP # / / / &! #0!#/ 0 " " 3# & + / . # %#1 !# - 3 A HP CS / & " 1. !$ #!# " + / / &#1 !#3 . # 0 0 !$# . 0 ).1.4. ! & # + &! !3 24 & ) A HP CS CSHP CA CAHP X 0,427457 0,002446 0,000252 1,53⋅⋅10–6 1,93⋅⋅10–7 X 0,053558 0,016121 0,006416 0,003020 X 0,485371 0,726714 0,443394 X 0,619995 0,975500 X 0,521807 CSHP CA X CAHP 128 # / / / &! #0!#/ 0 " " 3# & + / . # %#1 !# - 3 A / & " 1. !$ #!# " + / / &#1 !#3 . !$# . 0 ).1.5. ! & # + &! !3 48 & # 0 0 ) A HP CS CSHP CA CAHP X 0,809212 0,273196 0,258608 0,720845 0,466287 X 0,135105 0,125195 0,445276 0,454228 X 0,961776 0,120343 0,454676 X 0,106637 0,429891 X 0,598788 HP CS CSHP CA X CAHP # / / / &! #0!#/ 0 " " 3# & + / . # %#1 !# - 3 A HP CS / & " 1. !$ #!# " + / / &#1 !#3 . # 0 0 !$# . 0 ).1.6. ! & # + &! !3 72 & ) A HP CS CSHP CA CAHP X 0,231931 0,019024 0,025648 0,000003 0,000018 X 0,138616 0,127319 0,000048 0,002704 X 0,796009 0,000176 0,003202 X 0,000798 0,010910 X 0,406779 CSHP CA X CAHP 129 # / / / &! #0!#/ 0 " " 3# & + / . # %#1 & " 1. !$ #!# " !# - 3 A / + / &#1 !$# !#3 / . . 0 ).1.7. ! & # + &! !3 96 & # 0 0 ) A HP CS CSHP CA CAHP X 0,363595 0,562546 0,558155 0,406021 0,023176 X 0,338716 0,327023 0,001569 0,004267 X 0,977225 0,000996 0,006124 X 0,000694 0,005954 X 0,010894 HP CS CSHP CA X CAHP # 1/ 3/ 6/ 24 / / / / &! " 3# & + / #0!#/ 0 " . # %#1 / !# 5, 3 / & " 1. !$ #!# " + / /$ &#1 !$# !#3 +, /! %# / ! . 0 ).1.8. ! & # $ ! ,# /1 $ !# - 1/ 3/ 6/ 24 / 48 / 72 / 96 / X 0,043627 0,000039 2,29⋅⋅10–21 3,98⋅⋅10–20 < 1⋅⋅10–30 3,18⋅⋅10–21 X 0,015953 3,65⋅⋅10–18 3,08⋅⋅10–17 2,97⋅⋅10–28 1,34⋅⋅10–17 X 1,09⋅⋅10–14 3,61⋅⋅10–14 2,48⋅⋅10–22 6,12⋅⋅10–14 X 48 / 72 / 96 / 0,001076 2,02⋅⋅10–7 0,001180 X 0,036159 0,043618 X 0,645026 X 130 # / / / &! #0!#/ 0 " !# 5 . # / / " 3# & + / %#1 / & " 1. !$ #!# " !#3 H2O2, 3 0,8 + &#1 !$# +, + / /$ . 0 ).1.9. ! & # $ ! ,# /1 /! %# / ! $ !# 1/ 3/ 6/ 24 / 48 / 72 / 96 / X 0,186647 0,002891 0,000020 0,000010 1,21⋅⋅10–8 8,48⋅⋅10–8 X 0,029785 0,000168 0,000098 4,27⋅⋅10–7 0,000002 X 0,000627 0,000285 0,000003 0,000009 X 0,194536 0,007993 0,010948 X 0,057742 0,082679 X 0,755408 1/ 3/ 6/ 24 / 48 / 72 / 96 / X . # / / / &! #0!#/ 0 " !# 5 " 3# & + / / & " 1. &#1 . # %#1 !$ #!# " !#3 / / 2,5 + CuSO4, 3 + / !$# +, /$ ! 0 ).1.10. & # $ ! ,# /1 /! %# / ! $ !# - 1/ 3/ 6/ 24 / 1/ 3/ 6/ 24 / 48 / X 0,04270 0,000790 4,07⋅⋅10–10 1,23⋅⋅10–9 4,80⋅⋅10–13 3,74⋅⋅10–12 X 0,016192 1,12⋅⋅10–9 2,82⋅⋅10–9 7,29⋅⋅10–12 9,68⋅⋅10–11 X 5,20⋅⋅10–8 7,47⋅⋅10–8 2,22⋅⋅10–8 2,76⋅⋅10–7 X 0,088523 0,049088 0,104437 X 0,985001 0,885371 X 0,687528 48 / 72 / 96 / 72 / 96 / X 131 . # / / / &! #0!#/ 0 " " 3# & + / . # !# 5 %#1 / / $ 3/ X 0,020257 3/ X & " 1. !$ #!# " 2,5 + ! 6/ &#1 !$# !#3 0,8 + CuSO4 /! %# / 1/ 1/ / +, ! !# - 24 / 48 / + / 72 / 96 / 0,066612 6,30⋅⋅10–10 7,24⋅⋅10–9 2,94⋅⋅10–7 2,45⋅⋅10–8 8,26⋅⋅10–8 4,17⋅⋅10–7 0,000006 0,000006 X 0,188072 0,248382 0,253217 X 0,873099 0,849486 X 0,933262 X 24 / 48 / 72 / 96 / X . # / / / &! #0!#/ 0 " !# 5 . # / / " 3# & + / %#1 6/ 24 / / & " 1. !$ #!# " &#1 6/ !$# !#3 +, Cu(CH3COO)2, 3 2,5 + ! 3/ / 0,001423 3,12⋅⋅10–12 5,49⋅⋅10–11 1,02⋅⋅10–11 5,32⋅⋅10–12 6/ 1/ & # $ ! ,# /1 H2O2, 3 $ 0 ).1.11. $ + / ! 0 ).1.12. & # $ ! ,# /1 /$ /! %# / 72 / 96 / !# - 1/ 3/ X 1,47⋅⋅10–7 7,83⋅⋅10–17 8,52⋅⋅10–26 3,56⋅⋅10–23 1,74⋅⋅10–20 1,97⋅⋅10–20 X 2,71⋅⋅10–11 9,11⋅⋅10–25 2,39⋅⋅10–22 2,63⋅⋅10–18 2,47⋅⋅10–18 X 24 / 48 / 3,12⋅⋅10–20 2,41⋅⋅10–18 5,36⋅⋅10–13 4,02⋅⋅10–13 X 48 / 72 / 96 / 0,685642 0,588435 0,703352 X 0,411625 0,505665 X 0,645568 X 132 . # / / / &! #0!#/ 0 " !# 5 " 3# & + / . # %#1 / / 1/ 1/ 3/ 6/ 24 / X 3/ & " 1. !$ #!# " 2,5 + $ / !#3 Cu(CH3COO)2 /! %# / 6/ ! &#1 !$# +, 0,8 + $ !# - 24 / 48 / ! 0 ).1.13. & # $ ! ,# /1 H2O2, 3 72 / + / / 96 / 5,27⋅⋅10–10 2,85⋅⋅10–18 2,22⋅⋅10–27 1,95⋅⋅10–20 1,58⋅⋅10–16 1,22⋅⋅10–11 5,21⋅⋅10–8 1,88⋅⋅10–21 1,40⋅⋅10–15 2,54⋅⋅10–12 3,82⋅⋅10–7 X 3,48⋅⋅10–15 1,30⋅⋅10–10 3,05⋅⋅10–8 0,004322 X 0,647726 0,116336 0,000520 X 0,200961 0,009509 X 0,036457 X 48 / 72 / 96 / X 133 &*" 3 $* 2. .!,*%*4 % # % + &3!$*- !&' $*"#$(7 % +*$ $*8 , % %2 $0** "*2 % 4, 2 +, &9$ $! &*- , *$ (7 *$+ 1*& ,!$$ 4 *%" $*;, $1 $ &!1** !1 ! ! '!1* $$ 8 %& + . . $! 6 # ! $ ! ,# /1 . # %#1 1 3 6 24 48 ## + 2,5 + #0!#/ 0 0!/ / # / !# 5, 3 - ## " # . 0 - # . 0 $ /+ ), + / / !$! ## " !$!, + / / / !/ 0,2 1 2,5 5 0,111 0,099 0,122 0,120 0,147 ± 0,012 ± 0,005 ± 0,009 ± 0,008 ± 0,015 p = 0,1144 p = 0,1928 p = 0,2585 p = 0,0095 0,173 0,172 0,212 0,203 0,277 ± 0,019 ± 0,015 ± 0,011 ± 0,008 ± 0,023 p = 0,9368 p = 0,0120 p = 0,0270 p = 0,0004 0,308 0,462 0,764 0,944 1,254 ± 0,024 ± 0,038 ± 0,087 ± 0,146 ± 0,315 p = 0,0005 p = 0,0001 p = 0,0001 p = 0,0010 1,210 1,328 1,368 1,366 1,514 ± 0,037 ± 0,057 ± 0,062 ± 0,029 ± 0,035 p = 0,0133 p = 0,0047 p = 0,0006 p < 0,0001 1,379 1,383 1,356 1,336 1,266 ± 0,053 ± 0,052 ± 0,062 ± 0,040 ± 0,097 p = 0,9177 p = 0,5932 p = 0,2429 p = 0,0869 #- / / / &! !# - 0!/ / # / + (2,38 +, CuSO4 #0!#/ 0 - 0!/ / # / !$#!# ! !3 %/ / # !+ !#3 #$ 0 /! %# /% !# -, & !$ #!# " 0 ).2.1 - 48 ! +!#/ . " 3# & + / # . 0 ## " ##1! / !$!). 134 & " /# !$!#1 /! %# $! M ± SD #/ 3 - - (.!3 $ . - . $! 6 # ! . # $ ! ,# /1 3 %#1 #$ 0 + / / /! %# /% !# -, & 1 3 6 24 48 ## + 2,5 + #0!#/ 0 + (2,38 +, CuSO4 + 0,8 + 0!/ / # / - !# 5, H2O2 # . 0 # . 0 $ /+ ), ## " ## " !$! !$!, + / / / !/ 0,2 1 2,5 5 0,158 0,160 0,163 0,230 0,340 ± 0,021 ± 0,010 ± 0,011 ± 0,028 ± 0,035 p = 0,8691 p = 0,6878 p = 0,0063 p = 0,0001 0,273 0,309 0,406 0,471 0,609 ± 0,029 ± 0,018 ± 0,037 ± 0,048 ± 0,123 p = 0,0794 p = 0,0013 p = 0,0004 p = 0,0018 0,409 0,512 0,882 1,133 1,468 ± 0,041 ± 0,047 ± 0,117 ± 0,102 ± 0,209 p = 0,0163 p = 0,0003 p < 0,0001 p = 0,0001 1,279 1,275 1,311 1,350 1,438 ± 0,042 ± 0,021 ± 0,028 ± 0,058 ± 0,071 p = 0,8703 p = 0,2518 p = 0,0946 p = 0,0084 1,388 1,380 1,327 1,305 1,408 ± 0,041 ± 0,066 ± 0,055 ± 0,085 ± 0,052 p = 0,8437 p = 0,1257 p = 0,1291 p = 0,5679 #- / / / &! !# - 0!/ / # / !#3 #0!#/ 0 - 0!/ / # / !$#!# ! !3 %/ / # !+ !$ #!# " 0 ).2.2 - 48 ! +!#/ . " 3# & + / # . 0 ## " ##1! / !$!). 135 & " /# !$!#1 /! %# $! M ± SD #/ 3 - - (.!3 $ . - &*" 3 $* 3. <::*1* $ ( "*$ 8$ 8 $1 $ &!1* 8 !&' $*"#$(7 2& + ,,7 + : %:! *+*"7 "*$ ,(7 "*2 % 4 * 8** 0 !#/1 #1 #!"# " !$ #!# " H2O2- #$ 0 ## + +, !# 5 #!" $ / ,# / " / / / &! *&% $! 4 3+ *%" $*- $1 $ &!1* 8 "*2*+!. ! -0 !#3 && "-1** ) # r +!6$ $ ! ,# /1 37 , $! 6 # !+ #/ ## + #0!#/ 0 !" . # Cu(CH3COO)2 + $ 3 # !+ " 3# & + / p. . /! %# /% !# -, & %- / !# ! #$ 0 0 ).3.1 ## ! ) 1 r = 0,9801 (p = 0,020) r = 0,9898 (p = 0,010) 3 r = 0,9825 (p = 0,017) r = 0,9960 (p = 0,004) 24 r = 0,9752 (p = 0,025) r = 0,9590 (p = 0,041) 48 r = 0,9796 (p = 0,020) r = 0,9849 (p = 0,015) 96 r = 0,9819 (p = 0,018) r = 0,9778 (p = 0,022) 136 &*" 3 $* 4. "*-$* ' : &$ 9 &!% , &! $! %$ , MES – NaOH $! % + &3!$* !&' $*"#$(7 % +*$ $*8 , % %2 $0** "*2 % 4, 2 +, &9$ %2 $ !$$ 4 *$+ 1*& ,!$$ 4 (7 *%" $*;. . $! 6 # ! , / . # %#1 !$ #!# " !##1 # $ / ## " + (2,38 +, $! (– MES) NaOH (20 + , pH 6,8) (+ MES), 3 # 0 0 - !#3 + / / . /! %# /% ! ) 1 # . *! # + 0 ).4.1 $ /+ ), / ! MES– # 0 0 $ !# - !# -, & 3 6 24 – MES + MES – MES + MES – MES + MES – MES + MES / !# ! 0,121 0,152 0,144 0,272 0,180 0,279 0,889 1,082 ± 0,024 ± 0,016 ± 0,011 ± 0,023 ± 0,017 ± 0,029 ± 0,034 ± 0,077 p = 0,0752 2,5 + CuSO4 p = 0,0001 + 0,8 + CuSO4 H2O2 0,188 0,182 0,340 0,298 0,417 1,217 1,658 ± 0,023 ± 0,022 ± 0,025 ± 0,042 ± 0,015 ± 0,031 ± 0,033 ± 0,106 p = 0,0006 Cu(CH3COO)2 Cu(CH3COO)2 + 0,8 + H2O2 0,271 0,452 0,402 0,764 1,277 1,542 ± 0,012 ± 0,023 ± 0,024 ± 0,051 ± 0,051 ± 0,083 ± 0,054 ± 0,062 p = 0,0007 p = 0,0003 p = 0,0007 0,160 0,183 0,302 0,399 0,569 0,624 1,238 1,261 ± 0,033 ± 0,032 ± 0,011 ± 0,024 ± 0,040 ± 0,039 ± 0,023 ± 0,038 p = 0,0965 p = 0,0003 p = 0,3403 0,322 0,328 0,551 0,478 0,789 0,817 1,281 1,223 ± 0,020 ± 0,030 ± 0,032 ± 0,044 ± 0,045 ± 0,049 ± 0,056 ± 0,040 p = 0,3236 !$#!# ! !3 %/ / # !+ p = 0,0002 0,215 p = 0,3556 2,5 + p = 0,0005 0,165 p = 0,0084 2,5 + p = 0,0037 0,118 p = 0,0046 2,5 + p = 0,0011 #- / / / &! 48 p = 0,0364 ! +!#/ . " 3# & + / ##1! / & " p = 0,4322 p = 0,1429 $! M ± SD !$!#1 $! 6 # - . # %#1 3 !$ - #!# " !#3 +, , / !## " # MES–NaOH-. *! ! (+ MES), / / - , !#3 +, , / !## " # $ / 137 ## " $! (– MES). &*" 3 $* 5. "*-$* ' : &$(7 &!% , & , (& *%" $! % + &3!$* 2 +, &9$ 6,0) $! %$ , !&' $ ,(7 !&' $*"#$(7 % +*$ $*8 , % %2 $0** "*2 % 4, (7 *$+ 1*& ,!$$ 4 *%" $*;. . $! 6 # ! . # $ ! ,# /1 # # ! /! %# /% ! 0!/ /#1", 10 + 0 # /#1", 2+ 0 # /#1", 10 + / /#1", 2 + / /#1", 10 + !/ . . # 1 / !# -, & 3 6 24 0,176 ± 0,021 0,259 ± 0,030 0,421 ± 0,022 1,261 ± 0,049 0,190 ± 0,018 0,446 ± 0,088 1,045 ± 0,201 1,719 ± 0,033 p = 0,3504 p = 0,0069 p = 0,0008 p < 0,0001 0,252 ± 0,019 0,820 ± 0,041 1,518 ± 0,082 1,818 ± 0,099 p = 0,0017 p < 0,0001 p < 0,0001 p = 0,0001 0,198 ± 0,043 0,373 ± 0,070 0,375 ± 0,042 1,090 ± 0,066 p = 0,3933 p = 0,0242 p = 0,1004 p = 0,0059 0,198 ± 0,016 0,318 ± 0,022 0,407 ± 0,026 0,962 ± 0,083 p = 0,1466 p = 0,0193 p = 0,4425 p = 0,0008 0,185 ± 0,034 0,308 ± 0,036 0,342 ± 0,026 1,060 ± 0,044 p = 0,6682 p = 0,0815 p = 0,0035 p = 0,0009 0,125 ± 0,019 0,221 ± 0,015 0,270 ± 0,021 0,152 ± 0,031 p = 0,0113 p = 0,0640 p = 0,0001 p < 0,0001 #- / / / &! !#3 !# 5 H2O2 $ $ /+ ), 1 !$#!# ! !3 %/ / 54!" / + (2,38 +, CuSO4 + 0,8 + / / 0!/ /#1", 2 + !$ #!# " !#3 ## + 2,5 + . *! #1 #!/ # !+ !$ #!# " #$ 0 / / 2 *! #1" %#1 0 ).5.1 48 ! +!#/ . ##1! " $ / ! # / +, 0! . *! # , , / / / & " $! 6 # - !## " # , // +. 138 $! M ± SD !$!#1 # ! , . # 3 ## , #!3 . *! !## " 3 - %#1 - / !/ /!#3 . $! 6 # ! . # $ ! ,# /1 #$ 0 / / 2 *! #1" %#1 + (2,38 +, # # ! /! %# /% ! 0!/ /#1", 10 + 0 # /#1", 2+ 0 # /#1", 10 + / /#1", 2 + / /#1", 10 + !/ . / !# -, & 3 6 24 0,314 ± 0,019 0,569 ± 0,036 0,769 ± 0,018 1,254 ± 0,044 0,395 ± 0,029 0,662 ± 0,033 0,835 ± 0,043 1,281 ± 0,026 p = 0,0034 p = 0,0089 p = 0,0299 p = 0,3314 0,422 ± 0,023 1,093 ± 0,045 1,089 ± 0,029 1,258 ± 0,030 p = 0,0004 p < 0,0001 p < 0,0001 p = 0,8855 0,280 ± 0,017 0,230 ± 0,039 0,218 ± 0,020 1,299 ± 0,038 p = 0,0372 p < 0,0001 p < 0,0001 p = 0,1726 0,303 ± 0,024 0,317 ± 0,023 0,301 ± 0,025 1,108 ± 0,040 p = 0,4993 p < 0,0001 p < 0,0001 p = 0,0027 0,238 ± 0,019 0,253 ± 0,051 0,278 ± 0,012 1,144 ± 0,076 p = 0,0013 p = 0,0001 p < 0,0001 p = 0,0462 0,272 ± 0,024 0,188 ± 0,022 0,164 ± 0,024 0,173 ± 0,037 p = 0,0336 p < 0,0001 p < 0,0001 p < 0,0001 #- / / / &! !#3 !# 5 H2O2 . # 1 $ $ /+ ), 1 !$#!# ! !3 %/ / 54!" / / / 0!/ /#1", 2 + !$ #!# " !#3 Cu(CH3COO)2 + 0,8 + ## + 2,5 + . *! #1 #!/ # !+ !$ #!# " 0 ).5.2 48 ! +!#/ . ##1! " $ / ! # / +, 0! . *! # , , / / / & " $! 6 # - !## " # , // +. 139 $! M ± SD !$!#1 # ! , . # 3 ## , #!3 . *! !## " 3 - %#1 - / !/ /!#3